U72E3_ARATH
ID U72E3_ARATH Reviewed; 481 AA.
AC O81498;
DT 31-MAY-2011, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 1.
DT 03-AUG-2022, entry version 120.
DE RecName: Full=UDP-glycosyltransferase 72E3 {ECO:0000303|PubMed:11042215};
DE EC=2.4.1.111 {ECO:0000269|PubMed:15907484, ECO:0000269|PubMed:24667164};
DE AltName: Full=Hydroxycinnamate 4-beta-glucosyltransferase UGT72E3 {ECO:0000305};
DE EC=2.4.1.126 {ECO:0000269|PubMed:11042211, ECO:0000269|PubMed:15907484, ECO:0000269|PubMed:24667164};
GN Name=UGT72E3 {ECO:0000303|PubMed:11042215};
GN OrderedLocusNames=At5g26310 {ECO:0000312|Araport:AT5G26310};
GN ORFNames=F9D12.4 {ECO:0000312|EMBL:AAC26233.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Bautista V.R., Kim C.J., Chen H., Wu S.Y., De Los Reyes C., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP GENE FAMILY.
RX PubMed=11042215; DOI=10.1074/jbc.m007447200;
RA Li Y., Baldauf S., Lim E.K., Bowles D.J.;
RT "Phylogenetic analysis of the UDP-glycosyltransferase multigene family of
RT Arabidopsis thaliana.";
RL J. Biol. Chem. 276:4338-4343(2001).
RN [5]
RP FUNCTION, CATALYTIC ACTIVITY, AND BIOPHYSICOCHEMICAL PROPERTIES.
RX PubMed=11042211; DOI=10.1074/jbc.m007263200;
RA Lim E.K., Li Y., Parr A., Jackson R., Ashford D.A., Bowles D.J.;
RT "Identification of glucosyltransferase genes involved in sinapate
RT metabolism and lignin synthesis in Arabidopsis.";
RL J. Biol. Chem. 276:4344-4349(2001).
RN [6]
RP FUNCTION, AND CATALYTIC ACTIVITY.
RX PubMed=15907484; DOI=10.1016/j.febslet.2005.04.016;
RA Lim E.K., Jackson R.G., Bowles D.J.;
RT "Identification and characterisation of Arabidopsis glycosyltransferases
RT capable of glucosylating coniferyl aldehyde and sinapyl aldehyde.";
RL FEBS Lett. 579:2802-2806(2005).
RN [7]
RP TISSUE SPECIFICITY.
RX PubMed=16995900; DOI=10.1111/j.1365-313x.2006.02872.x;
RA Lanot A., Hodge D., Jackson R.G., George G.L., Elias L., Lim E.K.,
RA Vaistij F.E., Bowles D.J.;
RT "The glucosyltransferase UGT72E2 is responsible for monolignol 4-O-
RT glucoside production in Arabidopsis thaliana.";
RL Plant J. 48:286-295(2006).
RN [8]
RP FUNCTION, AND CATALYTIC ACTIVITY.
RX PubMed=24667164; DOI=10.1016/j.phytochem.2014.03.003;
RA Chu Y., Kwon T., Nam J.;
RT "Enzymatic and metabolic engineering for efficient production of syringin,
RT sinapyl alcohol 4-O-glucoside, in Arabidopsis thaliana.";
RL Phytochemistry 102:55-63(2014).
CC -!- FUNCTION: Involved in the O-glucosylation of monolignols (alcohol
CC monomers of lignin) (PubMed:11042211, PubMed:15907484,
CC PubMed:24667164). Glucosylates coniferyl alcohol to form coniferyl
CC alcohol 4-O-glucoside (PubMed:15907484, PubMed:24667164). Glucosylates
CC sinapyl alcohol to form sinapyl alcohol 4-O-glucoside (PubMed:11042211,
CC PubMed:15907484, PubMed:24667164). Possesses low activity with sinapate
CC as substrate (PubMed:11042211, PubMed:15907484).
CC {ECO:0000269|PubMed:11042211, ECO:0000269|PubMed:15907484,
CC ECO:0000269|PubMed:24667164}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(E)-4-coumarate + UDP-alpha-D-glucose = 4-O-(beta-D-glucosyl)-
CC trans-4-coumarate + H(+) + UDP; Xref=Rhea:RHEA:21636,
CC ChEBI:CHEBI:12876, ChEBI:CHEBI:15378, ChEBI:CHEBI:58223,
CC ChEBI:CHEBI:58885, ChEBI:CHEBI:79066; EC=2.4.1.126;
CC Evidence={ECO:0000269|PubMed:11042211, ECO:0000269|PubMed:15907484,
CC ECO:0000269|PubMed:24667164};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(E)-sinapyl alcohol + UDP-alpha-D-glucose = 4-O-(beta-D-
CC glucosyl)-trans-4-sinapoyl alcohol + H(+) + UDP;
CC Xref=Rhea:RHEA:57460, ChEBI:CHEBI:9380, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:58223, ChEBI:CHEBI:58885, ChEBI:CHEBI:64557;
CC Evidence={ECO:0000269|PubMed:11042211, ECO:0000269|PubMed:15907484,
CC ECO:0000269|PubMed:24667164};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(E)-coniferol + UDP-alpha-D-glucose = 4-O-(beta-D-glucosyl)-
CC trans-coniferol + H(+) + UDP; Xref=Rhea:RHEA:23944,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:16220, ChEBI:CHEBI:17745,
CC ChEBI:CHEBI:58223, ChEBI:CHEBI:58885; EC=2.4.1.111;
CC Evidence={ECO:0000269|PubMed:15907484, ECO:0000269|PubMed:24667164};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=E-sinapate + UDP-alpha-D-glucose = 4-O-(beta-D-glucosyl)-
CC trans-sinapate + H(+) + UDP; Xref=Rhea:RHEA:57456, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:30023, ChEBI:CHEBI:58223, ChEBI:CHEBI:58885,
CC ChEBI:CHEBI:141763; Evidence={ECO:0000269|PubMed:11042211,
CC ECO:0000269|PubMed:15907484};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(E)-coniferaldehyde + UDP-alpha-D-glucose = 4-O-(beta-D-
CC glucosyl)-4-trans-coniferyl aldehyde + H(+) + UDP;
CC Xref=Rhea:RHEA:57708, ChEBI:CHEBI:15378, ChEBI:CHEBI:16547,
CC ChEBI:CHEBI:58223, ChEBI:CHEBI:58885, ChEBI:CHEBI:136949;
CC Evidence={ECO:0000269|PubMed:15907484};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(E)-sinapaldehyde + UDP-alpha-D-glucose = 4-O-(beta-D-
CC glucosyl)-4-trans-sinapoyl aldehyde + H(+) + UDP;
CC Xref=Rhea:RHEA:57712, ChEBI:CHEBI:15378, ChEBI:CHEBI:27949,
CC ChEBI:CHEBI:58223, ChEBI:CHEBI:58885, ChEBI:CHEBI:142126;
CC Evidence={ECO:0000269|PubMed:15907484};
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=180 uM for sinapate {ECO:0000269|PubMed:11042211};
CC KM=850 uM for sinapyl alcohol {ECO:0000269|PubMed:11042211};
CC -!- TISSUE SPECIFICITY: Expressed in seedlings and roots, and at lower
CC levels in flowers and siliques. {ECO:0000269|PubMed:16995900}.
CC -!- SIMILARITY: Belongs to the UDP-glycosyltransferase family.
CC {ECO:0000305}.
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DR EMBL; AF077407; AAC26233.1; -; Genomic_DNA.
DR EMBL; CP002688; AED93550.1; -; Genomic_DNA.
DR EMBL; BT030376; ABO38789.1; -; mRNA.
DR PIR; T01850; T01850.
DR RefSeq; NP_198003.1; NM_122532.3.
DR AlphaFoldDB; O81498; -.
DR SMR; O81498; -.
DR STRING; 3702.AT5G26310.1; -.
DR CAZy; GT1; Glycosyltransferase Family 1.
DR PaxDb; O81498; -.
DR PRIDE; O81498; -.
DR ProteomicsDB; 228590; -.
DR EnsemblPlants; AT5G26310.1; AT5G26310.1; AT5G26310.
DR GeneID; 832700; -.
DR Gramene; AT5G26310.1; AT5G26310.1; AT5G26310.
DR KEGG; ath:AT5G26310; -.
DR Araport; AT5G26310; -.
DR TAIR; locus:2151059; AT5G26310.
DR eggNOG; KOG1192; Eukaryota.
DR HOGENOM; CLU_001724_3_0_1; -.
DR InParanoid; O81498; -.
DR OMA; SANHGFH; -.
DR OrthoDB; 508327at2759; -.
DR PhylomeDB; O81498; -.
DR BioCyc; ARA:AT5G26310-MON; -.
DR BioCyc; MetaCyc:AT5G26310-MON; -.
DR SABIO-RK; O81498; -.
DR PRO; PR:O81498; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; O81498; baseline and differential.
DR Genevisible; O81498; AT.
DR GO; GO:0047209; F:coniferyl-alcohol glucosyltransferase activity; IDA:TAIR.
DR GO; GO:0102359; F:daphnetin 4-O-beta-glucosyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0102361; F:esculetin 4-O-beta-glucosyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0047218; F:hydroxycinnamate 4-beta-glucosyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0102952; F:UDP-glucose:coniferaldehyde 4-beta-D-glucosyltransferase activity; IEA:RHEA.
DR GO; GO:0102956; F:UDP-glucose:sinapaldehyde 4-beta-D-glucosyltransferase activity; IEA:RHEA.
DR CDD; cd03784; GT1_Gtf-like; 1.
DR InterPro; IPR002213; UDP_glucos_trans.
DR InterPro; IPR035595; UDP_glycos_trans_CS.
DR Pfam; PF00201; UDPGT; 1.
DR PROSITE; PS00375; UDPGT; 1.
PE 1: Evidence at protein level;
KW Glycosyltransferase; Reference proteome; Transferase.
FT CHAIN 1..481
FT /note="UDP-glycosyltransferase 72E3"
FT /id="PRO_0000409075"
FT BINDING 272
FT /ligand="UDP-alpha-D-glucose"
FT /ligand_id="ChEBI:CHEBI:58885"
FT /evidence="ECO:0000250"
FT BINDING 346..348
FT /ligand="UDP-alpha-D-glucose"
FT /ligand_id="ChEBI:CHEBI:58885"
FT /evidence="ECO:0000250"
FT BINDING 363..371
FT /ligand="UDP-alpha-D-glucose"
FT /ligand_id="ChEBI:CHEBI:58885"
FT /evidence="ECO:0000250"
FT BINDING 385..388
FT /ligand="UDP-alpha-D-glucose"
FT /ligand_id="ChEBI:CHEBI:58885"
FT /evidence="ECO:0000250"
SQ SEQUENCE 481 AA; 52904 MW; 5766E0877F0A1583 CRC64;
MHITKPHAAM FSSPGMGHVL PVIELAKRLS ANHGFHVTVF VLETDAASVQ SKLLNSTGVD
IVNLPSPDIS GLVDPNAHVV TKIGVIMREA VPTLRSKIVA MHQNPTALII DLFGTDALCL
AAELNMLTYV FIASNARYLG VSIYYPTLDE VIKEEHTVQR KPLTIPGCEP VRFEDIMDAY
LVPDEPVYHD LVRHCLAYPK ADGILVNTWE EMEPKSLKSL QDPKLLGRVA RVPVYPVGPL
CRPIQSSTTD HPVFDWLNKQ PNESVLYISF GSGGSLTAQQ LTELAWGLEE SQQRFIWVVR
PPVDGSSCSD YFSAKGGVTK DNTPEYLPEG FVTRTCDRGF MIPSWAPQAE ILAHQAVGGF
LTHCGWSSTL ESVLCGVPMI AWPLFAEQNM NAALLSDELG ISVRVDDPKE AISRSKIEAM
VRKVMAEDEG EEMRRKVKKL RDTAEMSLSI HGGGSAHESL CRVTKECQRF LECVGDLGRG
A
