ID   TTDB_ECOLI              Reviewed;         201 AA.
AC   P0AC35; P05851; P33131; Q2M9E3;
DT   08-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT   08-NOV-2005, sequence version 1.
DT   03-AUG-2022, entry version 103.
DE   RecName: Full=L(+)-tartrate dehydratase subunit beta;
DE            Short=L-TTD beta;
DE            EC=4.2.1.32;
GN   Name=ttdB; Synonyms=ygjB; OrderedLocusNames=b3062, JW3034;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], CATALYTIC ACTIVITY, AND SUBUNIT.
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=8371115; DOI=10.1099/00221287-139-7-1523;
RA   Reaney S.K., Begg C., Bungard S.I., Guest J.R.;
RT   "Identification of the L-tartrate dehydratase genes (ttdA and ttdB) of
RT   Escherichia coli and evolutionary relationship with the class I fumarase
RT   genes.";
RL   J. Gen. Microbiol. 139:1523-1530(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3297921; DOI=10.1016/0378-1119(87)90303-9;
RA   Nesin M., Lupski J.R., Svec P., Godson G.N.;
RT   "Possible new genes as revealed by molecular analysis of a 5-kb Escherichia
RT   coli chromosomal region 5' to the rpsU-dnaG-rpoD macromolecular-synthesis
RT   operon.";
RL   Gene 51:149-161(1987).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [5]
RP   INDUCTION.
RX   PubMed=16804186; DOI=10.1099/mic.0.28753-0;
RA   Oshima T., Biville F.;
RT   "Functional identification of ygiP as a positive regulator of the ttdA-
RT   ttdB-ygjE operon.";
RL   Microbiology 152:2129-2135(2006).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(2R,3R)-tartrate = H2O + oxaloacetate; Xref=Rhea:RHEA:15413,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:16452, ChEBI:CHEBI:30924; EC=4.2.1.32;
CC         Evidence={ECO:0000269|PubMed:8371115};
CC   -!- SUBUNIT: Heterotetramer of two alpha and two beta subunits.
CC       {ECO:0000305|PubMed:8371115}.
CC   -!- INDUCTION: Induced by tartrate, via TtdR.
CC       {ECO:0000269|PubMed:16804186}.
CC   -!- SIMILARITY: Belongs to the class-I fumarase family. {ECO:0000305}.
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DR   EMBL; L14781; AAA03062.1; -; Unassigned_DNA.
DR   EMBL; M16194; AAA72574.1; -; Genomic_DNA.
DR   EMBL; U28379; AAA89142.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC76098.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAE77113.1; -; Genomic_DNA.
DR   PIR; D65094; QQECRZ.
DR   RefSeq; NP_417534.1; NC_000913.3.
DR   RefSeq; WP_000722957.1; NZ_STEB01000001.1.
DR   AlphaFoldDB; P0AC35; -.
DR   SMR; P0AC35; -.
DR   BioGRID; 4261199; 429.
DR   ComplexPortal; CPX-5912; L-tartrate dehydratase complex.
DR   IntAct; P0AC35; 2.
DR   STRING; 511145.b3062; -.
DR   PaxDb; P0AC35; -.
DR   PRIDE; P0AC35; -.
DR   DNASU; 947568; -.
DR   EnsemblBacteria; AAC76098; AAC76098; b3062.
DR   EnsemblBacteria; BAE77113; BAE77113; BAE77113.
DR   GeneID; 67414991; -.
DR   GeneID; 947568; -.
DR   KEGG; ecj:JW3034; -.
DR   KEGG; eco:b3062; -.
DR   PATRIC; fig|511145.12.peg.3156; -.
DR   EchoBASE; EB1157; -.
DR   eggNOG; COG1838; Bacteria.
DR   HOGENOM; CLU_098588_0_0_6; -.
DR   InParanoid; P0AC35; -.
DR   OMA; IYHCGPL; -.
DR   PhylomeDB; P0AC35; -.
DR   BioCyc; EcoCyc:TTDB-MON; -.
DR   BioCyc; MetaCyc:TTDB-MON; -.
DR   PRO; PR:P0AC35; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:1902494; C:catalytic complex; IC:ComplexPortal.
DR   GO; GO:0008730; F:L(+)-tartrate dehydratase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009408; P:response to heat; IMP:EcoCyc.
DR   GO; GO:1901276; P:tartrate catabolic process; IC:ComplexPortal.
DR   Gene3D; 3.20.130.10; -; 1.
DR   InterPro; IPR004647; Fe-S_hydro-lyase_TtdB-typ_cat.
DR   InterPro; IPR036660; Fe-S_hydroAse_TtdB_cat_sf.
DR   Pfam; PF05683; Fumerase_C; 1.
DR   SUPFAM; SSF117457; SSF117457; 1.
DR   TIGRFAMs; TIGR00723; ttdB_fumA_fumB; 1.
PE   1: Evidence at protein level;
KW   Lyase; Reference proteome.
FT   CHAIN           1..201
FT                   /note="L(+)-tartrate dehydratase subunit beta"
FT                   /id="PRO_0000195664"
FT   ACT_SITE        37
FT                   /evidence="ECO:0000255"
FT   CONFLICT        80..85
FT                   /note="SMRMES -> QYAYGK (in Ref. 1 and 2)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        165
FT                   /note="V -> A (in Ref. 1 and 2)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   201 AA;  22679 MW;  E81321CD19A8BE5C CRC64;
     MKKILTTPIK AEDLQDIRVG DVIYLTGTLV TCRDVCHRRL IELKRPIPYD LNGKAIFHAG
     PIVRKNGDKW EMVSVGPTTS MRMESFEREF IEQTGVKLVV GKGGMGPLTE EGCQKFKALH
     VIFPAGCAVL AATQVEEIEE VHWTELGMPE SLWVCRVKEF GPLIVSIDTH GNNLIAENKK
     LFAERRDPIV EEICEHVHYI K