TRR1_CAEEL
ID TRR1_CAEEL Reviewed; 4064 AA.
AC G5EEV2; Q5CZ43; Q6A4L2;
DT 29-APR-2015, integrated into UniProtKB/Swiss-Prot.
DT 14-DEC-2011, sequence version 1.
DT 03-AUG-2022, entry version 78.
DE RecName: Full=Transcription-associated protein 1 {ECO:0000312|WormBase:C47D12.1a};
DE Short=Cel-trr-1 {ECO:0000303|PubMed:24098152};
DE Short=TRRAP-like protein 1 {ECO:0000312|WormBase:C47D12.1a};
GN Name=trr-1 {ECO:0000312|WormBase:C47D12.1a};
GN ORFNames=C47D12.1 {ECO:0000312|WormBase:C47D12.1a};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1] {ECO:0000312|EMBL:AAS65430.1}
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), FUNCTION, SUBCELLULAR LOCATION,
RP TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RX PubMed=15068795; DOI=10.1016/s1534-5807(04)00065-6;
RA Ceol C.J., Horvitz H.R.;
RT "A new class of C. elegans synMuv genes implicates a Tip60/NuA4-like HAT
RT complex as a negative regulator of Ras signaling.";
RL Dev. Cell 6:563-576(2004).
RN [2] {ECO:0000312|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [3] {ECO:0000305}
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=24098152; DOI=10.1371/journal.pgen.1003850;
RA Guo Y., Chen X., Ellis R.E.;
RT "Evolutionary change within a bipotential switch shaped the sperm/oocyte
RT decision in hermaphroditic nematodes.";
RL PLoS Genet. 9:E1003850-E1003850(2013).
CC -!- FUNCTION: Influences germ cell fate in hermaphrodites
CC (PubMed:24098152). Acts downstream of tra-2 and tra-3 and through the
CC Tip60 histone acetyltransferase complex to regulate germ cell fate
CC decisions (By similarity). Required for spermatogenesis and embryonic
CC development (By similarity). Acts with tra-2 to promote expression of
CC fog-3 and control male tail development (By similarity). Involved in
CC the negative regulation of vulval development (PubMed:15068795).
CC {ECO:0000250|UniProtKB:A8WTE8, ECO:0000269|PubMed:15068795,
CC ECO:0000269|PubMed:24098152}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:15068795}. Chromosome
CC {ECO:0000269|PubMed:15068795}. Note=Localized to condensed chromosomes
CC during pachytene and diakinesis stages of meiosis I in germ cells.
CC {ECO:0000269|PubMed:15068795}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=a {ECO:0000312|WormBase:C47D12.1a};
CC IsoId=G5EEV2-1; Sequence=Displayed;
CC Name=b {ECO:0000312|WormBase:C47D12.1b};
CC IsoId=G5EEV2-2; Sequence=VSP_057588;
CC Name=c {ECO:0000312|WormBase:C47D12.1c};
CC IsoId=G5EEV2-3; Sequence=VSP_057584, VSP_057585, VSP_057586,
CC VSP_057587;
CC -!- TISSUE SPECIFICITY: Expressed in germ cells and somatic cells.
CC {ECO:0000269|PubMed:15068795}.
CC -!- DEVELOPMENTAL STAGE: Expressed throughout development from one-cell
CC stage embryos onwards. {ECO:0000269|PubMed:15068795}.
CC -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown results in partial
CC sterility and the production of oocytes by 14% of males at 20 degees
CC Celsius and by 23% of males at 25 degrees Celsius (PubMed:15068795,
CC PubMed:24098152). At 25 degrees Celsius, 8% of males produce no mature
CC germ cells and hermaphrodites develop into females (PubMed:24098152).
CC {ECO:0000269|PubMed:15068795, ECO:0000269|PubMed:24098152}.
CC -!- SIMILARITY: Belongs to the PI3/PI4-kinase family. TRA1 subfamily.
CC {ECO:0000305}.
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DR EMBL; AY551966; AAS65430.1; -; mRNA.
DR EMBL; Z69902; CAA93765.2; -; Genomic_DNA.
DR EMBL; Z69902; CAH10779.1; -; Genomic_DNA.
DR EMBL; Z69902; CAI59119.1; -; Genomic_DNA.
DR PIR; T19997; T19997.
DR RefSeq; NP_001022030.1; NM_001026859.2.
DR RefSeq; NP_001022031.1; NM_001026860.4.
DR RefSeq; NP_001022032.1; NM_001026861.4.
DR STRING; 6239.C47D12.1a; -.
DR EPD; G5EEV2; -.
DR PaxDb; G5EEV2; -.
DR PeptideAtlas; G5EEV2; -.
DR EnsemblMetazoa; C47D12.1a.1; C47D12.1a.1; WBGene00007028.
DR EnsemblMetazoa; C47D12.1b.1; C47D12.1b.1; WBGene00007028.
DR EnsemblMetazoa; C47D12.1c.1; C47D12.1c.1; WBGene00007028.
DR UCSC; C47D12.1b; c. elegans.
DR WormBase; C47D12.1a; CE36692; WBGene00007028; trr-1. [G5EEV2-1]
DR WormBase; C47D12.1b; CE36693; WBGene00007028; trr-1. [G5EEV2-2]
DR WormBase; C47D12.1c; CE38054; WBGene00007028; trr-1. [G5EEV2-3]
DR eggNOG; KOG0889; Eukaryota.
DR InParanoid; G5EEV2; -.
DR OMA; NPIFAMD; -.
DR OrthoDB; 7189at2759; -.
DR PhylomeDB; G5EEV2; -.
DR PRO; PR:G5EEV2; -.
DR Proteomes; UP000001940; Chromosome II.
DR Bgee; WBGene00007028; Expressed in germ line (C elegans) and 4 other tissues.
DR GO; GO:0000793; C:condensed chromosome; IDA:WormBase.
DR GO; GO:0000123; C:histone acetyltransferase complex; ISS:WormBase.
DR GO; GO:0005634; C:nucleus; IDA:WormBase.
DR GO; GO:0004674; F:protein serine/threonine kinase activity; IBA:GO_Central.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0000077; P:DNA damage checkpoint signaling; IBA:GO_Central.
DR GO; GO:1904262; P:negative regulation of TORC1 signaling; IBA:GO_Central.
DR GO; GO:0040027; P:negative regulation of vulval development; IMP:WormBase.
DR GO; GO:0040010; P:positive regulation of growth rate; IMP:WormBase.
DR GO; GO:0006468; P:protein phosphorylation; IBA:GO_Central.
DR GO; GO:0000003; P:reproduction; IMP:WormBase.
DR GO; GO:0007283; P:spermatogenesis; IEA:UniProtKB-KW.
DR GO; GO:0000723; P:telomere maintenance; IBA:GO_Central.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR003152; FATC_dom.
DR InterPro; IPR011009; Kinase-like_dom_sf.
DR InterPro; IPR000403; PI3/4_kinase_cat_dom.
DR InterPro; IPR014009; PIK_FAT.
DR SMART; SM00146; PI3Kc; 1.
DR SUPFAM; SSF48371; SSF48371; 2.
DR SUPFAM; SSF56112; SSF56112; 1.
DR PROSITE; PS51189; FAT; 1.
DR PROSITE; PS51190; FATC; 1.
DR PROSITE; PS50290; PI3_4_KINASE_3; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Chromosome; Developmental protein; Differentiation;
KW Nucleus; Reference proteome; Repeat; Spermatogenesis; TPR repeat.
FT CHAIN 1..4064
FT /note="Transcription-associated protein 1"
FT /evidence="ECO:0000305"
FT /id="PRO_0000432833"
FT REPEAT 1346..1379
FT /note="TPR 1"
FT /evidence="ECO:0000255"
FT REPEAT 1677..1714
FT /note="TPR 2"
FT /evidence="ECO:0000255"
FT DOMAIN 2802..3413
FT /note="FAT"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00534"
FT REPEAT 2849..2882
FT /note="TPR 3"
FT /evidence="ECO:0000255"
FT DOMAIN 3684..4048
FT /note="PI3K/PI4K catalytic"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00269"
FT DOMAIN 4033..4064
FT /note="FATC"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00535"
FT REGION 531..562
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2659..2692
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 3690..3696
FT /note="G-loop"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00269"
FT REGION 3904..3912
FT /note="Catalytic loop"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00269"
FT REGION 3924..3952
FT /note="Activation loop"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00269"
FT VAR_SEQ 823..825
FT /note="Missing (in isoform c)"
FT /id="VSP_057584"
FT VAR_SEQ 952..954
FT /note="Missing (in isoform c)"
FT /id="VSP_057585"
FT VAR_SEQ 2325..2327
FT /note="Missing (in isoform c)"
FT /id="VSP_057586"
FT VAR_SEQ 2373..2375
FT /note="NCR -> K (in isoform c)"
FT /id="VSP_057587"
FT VAR_SEQ 4005..4007
FT /note="Missing (in isoform b)"
FT /id="VSP_057588"
SQ SEQUENCE 4064 AA; 466866 MW; A2C5733487225C61 CRC64;
MDPAMASPGY RSVQSDRSNH LTELETRIQN LADNSQRDDV KLKMLQEIWS TIENHFTLSS
HEKVVERLIL SFLQVFCNTS PQFIAENNTQ QLRKLMLEII LRLSNVEAMK HHSKEIIKQM
MRLITVENEE NANLAIKIVT DQGRSTGKMQ YCGEVSQIMV SFKTMVIDLT ASGRAGDMFN
IKEHKAPPST SSDEQVITEY LKTCYYQQTV LLNGTEGKPP LKYNMIPSAH QSTKVLLEVP
YLVIFFYQHF KTAIQTEALD FMRLGLDFLN VRVPDEDKLK TNQIITDDFV SAQSRFLSFV
NIMAKIPAFM DLIMQNGPLL VSGTMQMLER CPADLISVRR EVLMALKYFT SGEMKSKFFP
MLPRLIAEEV VLGTGFTAIE HLRVFMYQML ADLLHHMRNS IDYEMITHVI FVFCRTLHDP
NNSSQVQIMS ARLLNSLAES LCKMDSHDTF QTRDLLIEIL ESHVAKLKTL AVYHMPILFQ
QYGTEIDYEY KSYERDAEKP GMNIPKDTIR GVPKRRIRRL SIDSVEELEF LASEPSTSED
ADESGGDPNK LPPPTKEGKK TSPEAILTAM STMTPPPLAI VEARNLVKYI MHTCKFVTGQ
LRIARPSQDM YHCSKERDLF ERLLRYGVMC MDVFVLPTTR NQPQMHSSMR TKDEKDALES
LANVFTTIDH AIFREIFEKY MDFLIERIYN RNYPLQLMVN TFLVRNEVPF FASTMLSFLM
SRMKLLEVSN DKTMLYVKLF KIIFSAIGAN GSGLHGDKML TSYLPEILKQ STVLALTARE
PLNYFLLLRA LFRSIGGGAQ DILYGKFLQL LPNLLQFLNK LTNLQSCQHR IQMRELFVEL
CLTVPVRLSS LLPYLPLLMD PLVCAMNGSP NIVTQGLRTL ELCVDNLQPE YLLENMLPVR
GALMQGLWRV VSKAPDTSSM TAAFRILGKF GGANRKLLNQ PQILQVATLG DTVQSYINME
FSRMGLDGNH SIHLPLSELM RVVADQMRYP ADMILNPSPA MIPSTHMKKW CMELSKAVLL
AGLGSSGSPI TPSANLPKII KKLLEDFDPN NRTTEVYTCP RESDRELFVN ALLAMAYGIW
NKDGFRHVYS KFFIKVLRQF ALIGVLEYIG GNGWMRHAEE EGVLPLCLDS SVMVDALIIC
LSETSSSFII AGVMSLRHIN ETLSLTLPDI DQMSKVPMCK YLMEKVFKLC HGPAWYARSG
GINAIGYMIE SFPRKFVMDF VIDVVDSIME VILGTVEEIS SGSADSAYDC LKKMMRVYFI
KEEGQEEENL TLATIFVSAI SKHYFHSNER VREFAIGLMD HCMVHSRLAP SLDKFYYRFK
EFFEPELMRV LTTVPTMSLA DAGGSLDGVQ NYMFNCPDGF DFEKDMDMYK RYLSHLLDIA
QTDTFTLNQR NAFKKCETCP SHFLPPFPIT THIDSMRASA LQCLVIAYDR MKKQYIDKGI
ELGDEHKMIE ILALRSSKIT VDQVYESDES WRRLMTVLLR AVTDRETPEI AEKLHPSLLK
VSPISTIIIA TFGASYIRNI SGAGDDSDSD RHISYNDIMK FKCLVELNPK ILVTKMAVNL
ANQMVKYKMS DKISRILSVP SSFTEEELDD FEAEKMKGIR ELDMIGHTVK MLAGCPVTTF
TEQIIVDISR FAAHFEYAYS QDVLVNWIDD VTVILNKSPK DVWKFFLSRE SILDPARRSF
IRRIIVYQSS GPLRQEFMDT PEYFEKLIDL DDEENKDEDE RKIWDRDMFA FSIVDRISKS
CPEWLISPNS PIPRIKKLFS ETEFNERYVV RALTEVKKFQ EEIIVKRMTE HKYKVPKLIL
NTFLRYLRLN IYDYDLFIVI ASCFNGNFVT DLSFLREYLE TEVIPKVPLQ WRRELFLRIM
QKFDTDPQTA GTSMQHVKAL QYLVIPTLHW AFERYDTDEI VGTAPIDDSD SSMDVDPAGS
SDNLVARLTS VIDSHRNYLS DGMVIVFYQL CTLFVQNASE HIHNNNCKKQ GGRLRILMLF
AWPCLTMYNH QDPTMRYTGF FFLANIIERF TINRKIVLQV FHQLMTTYQQ DTRDQIRKAI
DILTPALRTR MEDGHLQILS HVKKILIEEC HNLQHVQHVF QMVVRNYRVY YHVRLELLTP
LLNGVQRALV MPNSVLEKFS WQTRRHAVEI CEMVIKWELF RTLKTDHIIS DEEALEVDKQ
LDKLRTASST DRFDFEEAHN KRDMPDAQRT IIKEHADVIV NMLVRFCMTF HQNSGSSSTS
QSGNHGVELT KKCQLLLRAA LRPSMWGEFV SFRLTMIEKF LSIPNDNALR NDISSTAYAN
TIQNAQHTLD MLCNIIPVMP KTSLMTMMRQ LQRPLIQCLN NGAQNFKMTR LVTQIVSRLL
EKTNVSVNGL DELEQLNQYI SRFLHEHFGS LLNCRNLSGP VLGVLGAFSL LRTICGHEPA
YLDHLMPSFV KVMERAAKEH LAYVANSQDG NMVKNFFPDV AELLCACMEL VRPRVDHISM
EIKRSIVGGI IAELIIKSNH DKIIQTSVKL LGAMISTQDM EFTILTVLPL LVRIQSIIVT
KFKNCKDLIA DYLVVVITVF ENSEYRNSEA GSRLWEGFFW GLKSSDPQTR EKFSIVWEKT
WPHMATVDIA HRMKYIMQNQ DWSKFKHAFW LKFALWGMLR TIAKRPTDPN NKRKKVILLN
CATPWRTIEY AAKLKDQPME VETEMKREEP EPMEVDEKDS QDDSKDAGEP KEKEKLTLEL
LLAGQQELLD EASNYDFADA LDTVSQITFA LNENQVTSKM WVVLFKSFWS SLSQSEIEDF
TALVVPFMSS GVHNNYQTGV QDSVLAVWLE AVGDAVHLPS RLIEFISSKH ECWHTGIRLL
ENHIWTIPKQ LNNTLLREMK VAPGLAGDIE TLESLGTLYN EISEFDQFAA IWERRAVFPD
TMRAMSAMQL GDMELAQSYL EKSMSSTYET LAPTINPNNT SNSEKHVSPI IDKEYDHWME
MYITNCSELL QWQNVADVCN GKDMQHVRGL INAASHIPDW NVVEECKSQI AGCIPPSFHL
DYTLFNLMST VMRMNENSSP THMKERCKIA IQECTEAHIS RWRALPSVVS YGHVKILQAM
NLVREIEEST DIRIALLEAP SNKVDQALMG DMKSLMKVFR NRTPTTSDDM GFVSTWYDWR
NQIHGMMLQR FEYWDKVGLN VAATGNQSIV PIHSMAQAQL AVAKHAKNLG FHNLTKDLLN
KLAGLTAIPM MDAQDKVCTY GKTLRDMANS AADERVKNEL LCEALEVLED VRIDDLQKDQ
VAALLYHRAN IHSVLDQAEN ADYTFSAASQ LVDLQNSVTT TGIKLMKNWG HHLYKRFFST
TVCKETGNNF GRQALACYFI AARVDNDIKA RKPIAKILWL SKHLNACGSH EVMNRVIKKQ
LHSLNLFNWL YWLPQLVTDV RYKPNSNFVL ILCKMAAAHP LQVFYHIREA VSVDDIDSVL
EEDYTDEQMS MDVSDEDCFA DDPPFDRILK ICLKYRPTDI RVFHRVLKEL DEMNETWVER
HLRHAICLKD QMFKDFSEQM DATFNEMQYS EDVTMMTLRW RKQLEEDLVY FQQNYNLDFL
EIRNKRKMIV TKGCMGVEKS QIMFEKELSQ VFTEPAGMQD EFDFVTNMTN MMVSQLDIHA
VDAPRPQGYI RIVLDWIRAI RRRFDRLPRR IPLESSSPYL ARFSHRTGCI EMPYDLLNVL
RAKNHTLMAS NQTGQYISML SRFEPNFEIV IKGGQVIRKI YIRGQTGKSA AFYLKKSVQD
EPTNRVPQMF KHLDHVLQTD RESARRHLHA PTVLQMRVGQ KTTLYEVASV QPYAMPPDCT
RNYPASQIDI VHPYDVLTAT FNGSYYPDDM VLHFFERFAQ SSSSIGQPLP TPTNQDGTVA
PPRLTEAHHI KNIIYEDFAR DMIPFRLLYD YLTARYPDPV MYYAMKKQLL HSLAVLSTIE
YHCNLTPMGP DQMMMTMNTG VLSNPSYRFE IRGGRSLHDI QHFGHEVPFR LTPNLSILVG
VAQDGDLLWS MAAASKCLMK KEPEVIMRPL VWDEFANNTD CDKSRLQVFA CHASNSYING
VASKLRNTNS ADAKLRKDDC VSLISRAKDS DNLARMPPTY HAWF
