TRF1B_ARATH
ID TRF1B_ARATH Reviewed; 1082 AA.
AC A8MQL1; F4I479; P93826; P93827; Q0WNI3;
DT 22-NOV-2017, integrated into UniProtKB/Swiss-Prot.
DT 04-DEC-2007, sequence version 1.
DT 25-MAY-2022, entry version 94.
DE RecName: Full=TNF receptor-associated factor homolog 1b {ECO:0000305};
DE AltName: Full=Protein MUTANT SNC1-ENHANCING 13 {ECO:0000303|PubMed:26867179};
GN Name=TRAF1B {ECO:0000303|PubMed:28351989};
GN Synonyms=MUSE13 {ECO:0000303|PubMed:26867179};
GN OrderedLocusNames=At1g04300 {ECO:0000312|Araport:AT1G04300};
GN ORFNames=F19P19.26 {ECO:0000312|EMBL:AAB70446.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, CLEAVAGE OF INITIATOR
RP METHIONINE [LARGE SCALE ANALYSIS], AND IDENTIFICATION BY MASS SPECTROMETRY
RP [LARGE SCALE ANALYSIS].
RX PubMed=22223895; DOI=10.1074/mcp.m111.015131;
RA Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T.,
RA Giglione C.;
RT "Comparative large-scale characterisation of plant vs. mammal proteins
RT reveals similar and idiosyncratic N-alpha acetylation features.";
RL Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012).
RN [5]
RP FUNCTION, SUBUNIT, INTERACTION WITH SNC1; RPS2 AND CPR1/CPR30, SUBCELLULAR
RP LOCATION, AND DISRUPTION PHENOTYPE.
RX PubMed=26867179; DOI=10.1016/j.chom.2016.01.005;
RA Huang S., Chen X., Zhong X., Li M., Ao K., Huang J., Li X.;
RT "Plant TRAF proteins regulate NLR immune receptor turnover.";
RL Cell Host Microbe 19:204-215(2016).
RN [6]
RP FUNCTION, INTERACTION WITH ATG6, SUBCELLULAR LOCATION, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=28351989; DOI=10.1105/tpc.17.00056;
RA Qi H., Xia F.N., Xie L.J., Yu L.J., Chen Q.F., Zhuang X.H., Wang Q., Li F.,
RA Jiang L., Xie Q., Xiao S.;
RT "TRAF family proteins regulate autophagy dynamics by modulating AUTOPHAGY
RT PROTEIN6 stability in Arabidopsis.";
RL Plant Cell 29:890-911(2017).
CC -!- FUNCTION: Functions redundantly with TRAF1A in the regulation of plant
CC immune response. Contributes to the turnover of the nucleotide-binding
CC domain and leucine-rich repeat-containing (NB-LRR) immune receptors
CC SNC1 and RPS2. May associate with an E3 ubiquitin-protein ligase
CC complex, which modulates ubiquitination and subsequent degradation of
CC NB-LRR immune sensors to maintain their homeostasis (PubMed:26867179).
CC Functions redundantly with TRAF1A in the regulation of autophagosome
CC formation. Required for SINAT1- and SINAT2-mediated ubiquitination and
CC destabilization of ATG6. Functions as molecular adapter that helps to
CC regulate autophagy by modulating ATG6 stability (PubMed:28351989).
CC {ECO:0000269|PubMed:26867179, ECO:0000269|PubMed:28351989}.
CC -!- SUBUNIT: Forms homooligomers. Interacts with SNC1, RPS2 and CPR1/CPR30
CC (PubMed:26867179). Interacts with ATG6 (PubMed:28351989).
CC {ECO:0000269|PubMed:26867179, ECO:0000269|PubMed:28351989}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:26867179,
CC ECO:0000269|PubMed:28351989}. Cell membrane
CC {ECO:0000269|PubMed:26867179}; Peripheral membrane protein
CC {ECO:0000305|PubMed:26867179}. Note=Predominantly expressed in the
CC cytoplasm (PubMed:26867179, PubMed:28351989). Associates with
CC starvation-induced autophagosomes in continuous darkness
CC (PubMed:28351989). {ECO:0000269|PubMed:26867179,
CC ECO:0000269|PubMed:28351989}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=A8MQL1-1; Sequence=Displayed;
CC Name=2;
CC IsoId=A8MQL1-2; Sequence=VSP_059231;
CC -!- DISRUPTION PHENOTYPE: No visible phenotype under normal growth
CC conditions, but the double mutant plants traf1a and traf1b, or muse13
CC and muse14 are extremely dwarf when grown at room temperature.
CC {ECO:0000269|PubMed:26867179}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAB70446.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC000104; AAB70446.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE27681.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE27682.1; -; Genomic_DNA.
DR EMBL; AK229458; BAF01316.1; -; mRNA.
DR PIR; E86174; E86174.
DR RefSeq; NP_001077454.1; NM_001083985.1. [A8MQL1-2]
DR RefSeq; NP_001077455.1; NM_001083986.1. [A8MQL1-1]
DR AlphaFoldDB; A8MQL1; -.
DR SMR; A8MQL1; -.
DR STRING; 3702.AT1G04300.3; -.
DR iPTMnet; A8MQL1; -.
DR PaxDb; A8MQL1; -.
DR PRIDE; A8MQL1; -.
DR ProteomicsDB; 228264; -. [A8MQL1-1]
DR EnsemblPlants; AT1G04300.2; AT1G04300.2; AT1G04300. [A8MQL1-2]
DR EnsemblPlants; AT1G04300.3; AT1G04300.3; AT1G04300. [A8MQL1-1]
DR GeneID; 839551; -.
DR Gramene; AT1G04300.2; AT1G04300.2; AT1G04300. [A8MQL1-2]
DR Gramene; AT1G04300.3; AT1G04300.3; AT1G04300. [A8MQL1-1]
DR KEGG; ath:AT1G04300; -.
DR Araport; AT1G04300; -.
DR TAIR; locus:2018279; AT1G04300.
DR eggNOG; ENOG502QW6P; Eukaryota.
DR HOGENOM; CLU_005068_0_0_1; -.
DR InParanoid; A8MQL1; -.
DR OrthoDB; 145806at2759; -.
DR PhylomeDB; A8MQL1; -.
DR PRO; PR:A8MQL1; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; A8MQL1; baseline and differential.
DR GO; GO:0005737; C:cytoplasm; IDA:TAIR.
DR GO; GO:0005829; C:cytosol; IDA:TAIR.
DR GO; GO:0005886; C:plasma membrane; IDA:TAIR.
DR GO; GO:1905037; P:autophagosome organization; IMP:TAIR.
DR GO; GO:0045087; P:innate immune response; IGI:TAIR.
DR CDD; cd00121; MATH; 1.
DR Gene3D; 2.60.210.10; -; 1.
DR InterPro; IPR002083; MATH/TRAF_dom.
DR InterPro; IPR008974; TRAF-like.
DR Pfam; PF00917; MATH; 1.
DR SMART; SM00061; MATH; 1.
DR PROSITE; PS50144; MATH; 1.
PE 1: Evidence at protein level;
KW Acetylation; Alternative splicing; Cell membrane; Coiled coil; Cytoplasm;
KW Membrane; Reference proteome.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0007744|PubMed:22223895"
FT CHAIN 2..1082
FT /note="TNF receptor-associated factor homolog 1b"
FT /id="PRO_0000442354"
FT DOMAIN 67..198
FT /note="MATH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00129"
FT REGION 1..54
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 358..388
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 440..666
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 697..762
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 780..800
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 812..841
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 858..889
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 446..507
FT /evidence="ECO:0000255"
FT COMPBIAS 14..50
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 440..455
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 469..509
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 564..666
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 713..737
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 2
FT /note="N-acetylalanine"
FT /evidence="ECO:0007744|PubMed:22223895"
FT VAR_SEQ 1..126
FT /note="MAEAVDEDSGVGRSLEESSNGQHSQAGEALSEWRSSGQVENGTPSTSPSYWD
FT IDDDDDYGLKPSELYGQYTWKIPKFSEITKREHRSNVFEAGGYKWYILIYPQGCDVCNH
FT LSLFLCVANYDKLLP -> MFVIISPCFSVLQIMINFFQVSIIVHLSAWSLFSLWIPSH
FT V (in isoform 2)"
FT /id="VSP_059231"
FT CONFLICT 324
FT /note="D -> G (in Ref. 3; BAF01316)"
FT /evidence="ECO:0000305"
FT CONFLICT 837
FT /note="H -> R (in Ref. 3; BAF01316)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1082 AA; 120444 MW; 95B9398A3BC750A3 CRC64;
MAEAVDEDSG VGRSLEESSN GQHSQAGEAL SEWRSSGQVE NGTPSTSPSY WDIDDDDDYG
LKPSELYGQY TWKIPKFSEI TKREHRSNVF EAGGYKWYIL IYPQGCDVCN HLSLFLCVAN
YDKLLPGSFA ILEAGWSQFA QFTISVLSQD LKKSKFSDTL HRFWKKEHDW GWKKFMELPK
LKDGFIDESG CLTIEAKVQV IRERVDRPFR CLDCGYRREL VRVYFQNVEQ ICRRFVEEKR
SKLGRLIEDK ARWTSFGVFW LGMDQNSRRR MCREKVDVIL KGVVKHFFVE KEVSSTLVMD
SLYSGLKALE GQTKNMKARS RLLDAKQLPA PIVSVDKDMF VLVDDVLLLL ERAALEPLPP
KDEKGRQNRT KDGNDGEEVN KEADERDERR LTELGRRTVE IFILSHIFST KIEVAHQEAI
ALKRQEELIR EEEEAWLAET EQRAKRGAAE REKKSKKKQA KQKRNKNKGK DKRKEEKVSF
ATHAKDLEEN QNQNQNDEEE KDSVTEKAQS SAEKPDTLGD VSDISDSVDG SADILQPDLE
DRDSSSVLWD TDALEIHPPS SEGSSRGRGI SISTPNGITE GKSHSTMDDS SSTCSNDSIR
SGVTNGSYQG NSLNFRNQKS PNKGKNQQVK AMTDAHSLAS ETDDQPSTLG TDPKGQNYSS
EASNVGESDW VVVSHIQEPE GSRNRIPVGR ERKTVQSIVN SVDMDRPKEK STAVLSSPRN
VAKNPSPLTQ TKPEKKSIST ADGIPNRKVL ATGPPSSSQV VLPSDIQSQT VGLRADMQKL
SAPKQPPATT ISRPSSAPII PAMRPSPITV SSSVQTTTSL PRSVSSAGRL GPDPSLHNQQ
TYTPQSYKNA IVGNSLGSSS SSFNHHPSSH GVVPTTLPSS SYSQAPTSSY QSSFPYSQDG
LLWTGRSPSS VNMGMYNNTY SPAVTSNRSL NHMDVQIAQQ QAQSMMTDEF PHLDIINDLL
EDEQCSNMVY NGSIFNPQPQ VFNGQYSSYH GELLSGGRTR SFGEEGLHYM ARGPYGTDGM
MPRQWQMTNM DLSLPAMRSN GMEDGTSSAA NYHHSYFGLD ASNPSFTSGI NGYTEFRPSN
GH
