TO401_DROME
ID TO401_DROME Reviewed; 344 AA.
AC Q9U4L6; A4V439; Q9W3P9;
DT 11-JAN-2001, integrated into UniProtKB/Swiss-Prot.
DT 11-JAN-2001, sequence version 2.
DT 03-AUG-2022, entry version 145.
DE RecName: Full=Mitochondrial import receptor subunit TOM40 homolog 1;
DE Short=dtom40;
DE AltName: Full=Male sterile protein 15;
DE AltName: Full=Translocase of outer membrane 40 kDa subunit homolog 1;
GN Name=Tom40; Synonyms=mit, ms(1)15; ORFNames=CG12157;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Head, Ovary, and Testis;
RA Xu E.Y., Wu C.-I.;
RT "A mutation in Drosophila mitochondrial membrane import protein disrupts
RT early germ cell differentiation.";
RL Submitted (JUL-1999) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [5]
RP TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=15304338; DOI=10.1016/j.febslet.2004.07.025;
RA Hwa J.J., Zhu A.J., Hiller M.A., Kon C.Y., Fuller M.T., Santel A.;
RT "Germ-line specific variants of components of the mitochondrial outer
RT membrane import machinery in Drosophila.";
RL FEBS Lett. 572:141-146(2004).
CC -!- FUNCTION: Channel-forming protein essential for import of protein
CC precursors into mitochondria. {ECO:0000250}.
CC -!- SUBUNIT: Forms part of the preprotein translocase complex of the outer
CC mitochondrial membrane (TOM complex). Interacts with mitochondrial
CC targeting sequences (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion outer membrane {ECO:0000250};
CC Multi-pass membrane protein {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Ubiquitously expressed. In the male germ line
CC expression is detected early in premeiotic spermatogonia and is
CC persistent to the end of meiosis. {ECO:0000269|PubMed:15304338}.
CC -!- DEVELOPMENTAL STAGE: Expressed both maternally and zygotically.
CC {ECO:0000269|PubMed:15304338}.
CC -!- SIMILARITY: Belongs to the Tom40 family. {ECO:0000305}.
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DR EMBL; AF165113; AAF20172.1; -; mRNA.
DR EMBL; AE014298; AAF46272.1; -; Genomic_DNA.
DR EMBL; AE014298; AAS65272.1; -; Genomic_DNA.
DR EMBL; AY118926; AAM50786.1; -; mRNA.
DR RefSeq; NP_001259313.1; NM_001272384.1.
DR RefSeq; NP_652003.2; NM_143746.5.
DR RefSeq; NP_996363.1; NM_206640.2.
DR AlphaFoldDB; Q9U4L6; -.
DR SMR; Q9U4L6; -.
DR BioGRID; 69425; 14.
DR DIP; DIP-18955N; -.
DR STRING; 7227.FBpp0305890; -.
DR TCDB; 1.B.8.2.2; the mitochondrial and plastid porin (mpp) family.
DR PaxDb; Q9U4L6; -.
DR PRIDE; Q9U4L6; -.
DR DNASU; 44978; -.
DR EnsemblMetazoa; FBtr0071073; FBpp0071031; FBgn0016041.
DR EnsemblMetazoa; FBtr0071074; FBpp0089237; FBgn0016041.
DR EnsemblMetazoa; FBtr0333748; FBpp0305890; FBgn0016041.
DR GeneID; 44978; -.
DR KEGG; dme:Dmel_CG12157; -.
DR UCSC; CG12157-RB; d. melanogaster.
DR CTD; 44978; -.
DR FlyBase; FBgn0016041; Tom40.
DR VEuPathDB; VectorBase:FBgn0016041; -.
DR eggNOG; KOG3296; Eukaryota.
DR GeneTree; ENSGT00390000003308; -.
DR HOGENOM; CLU_054399_0_0_1; -.
DR InParanoid; Q9U4L6; -.
DR OMA; SHQFAMG; -.
DR OrthoDB; 1346842at2759; -.
DR PhylomeDB; Q9U4L6; -.
DR BioGRID-ORCS; 44978; 1 hit in 3 CRISPR screens.
DR GenomeRNAi; 44978; -.
DR PRO; PR:Q9U4L6; -.
DR Proteomes; UP000000803; Chromosome X.
DR Bgee; FBgn0016041; Expressed in cleaving embryo and 49 other tissues.
DR ExpressionAtlas; Q9U4L6; baseline and differential.
DR Genevisible; Q9U4L6; DM.
DR GO; GO:0005742; C:mitochondrial outer membrane translocase complex; IBA:GO_Central.
DR GO; GO:0005739; C:mitochondrion; HDA:FlyBase.
DR GO; GO:0046930; C:pore complex; IEA:UniProtKB-KW.
DR GO; GO:0015288; F:porin activity; IEA:UniProtKB-KW.
DR GO; GO:0008320; F:protein transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0071456; P:cellular response to hypoxia; IMP:FlyBase.
DR GO; GO:0006811; P:ion transport; IEA:UniProtKB-KW.
DR GO; GO:0030150; P:protein import into mitochondrial matrix; IBA:GO_Central.
DR GO; GO:0006626; P:protein targeting to mitochondrion; ISS:UniProtKB.
DR CDD; cd07305; Porin3_Tom40; 1.
DR Gene3D; 2.40.160.10; -; 1.
DR InterPro; IPR023614; Porin_dom_sf.
DR InterPro; IPR027246; Porin_Euk/Tom40.
DR InterPro; IPR037930; Tom40.
DR PANTHER; PTHR10802; PTHR10802; 1.
DR Pfam; PF01459; Porin_3; 1.
PE 2: Evidence at transcript level;
KW Ion transport; Membrane; Mitochondrion; Mitochondrion outer membrane;
KW Porin; Protein transport; Reference proteome; Transmembrane;
KW Transmembrane beta strand; Transport.
FT CHAIN 1..344
FT /note="Mitochondrial import receptor subunit TOM40 homolog
FT 1"
FT /id="PRO_0000051530"
FT REGION 1..44
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 28..44
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 2
FT /note="G -> A (in Ref. 1; AAF20172)"
FT /evidence="ECO:0000305"
FT CONFLICT 13
FT /note="G -> D (in Ref. 1; AAF20172)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 344 AA; 36336 MW; 1EE945CBD18EDEFB CRC64;
MGNVLAASSG APGSGASNLG LGLQEPAPLP SNSGSLTESS SSAEGLDSLA AAKDAALENP
GTVEELHKKC KDIQAITFEG AKIMLNKGLS NHFQVSHTIN MSNVVPSGYR FGATYVGTKE
FSPTEAFPVL LGDIDPAGNL NANVIHQFSA RLRCKFASQI QESKVVASQL TTDYRGSDYT
LSLTVANPSI FTNSGVVVGQ YLQSVTPALA LGSELAYQFG PNVPGRQIAI MSVVGRYTAG
SSVWSGTLGQ SGLHVCYYQK ASDQLQIGAE VETSLRMQES VATLAYQIDL PKANLVFRGG
IDSNWQIFGV LEKRLAPLPF TLALSGRMNH VKNNFRLGCG LMIG
