BTG4_MOUSE
ID BTG4_MOUSE Reviewed; 250 AA.
AC O70552; Q925T9;
DT 27-APR-2001, integrated into UniProtKB/Swiss-Prot.
DT 18-SEP-2013, sequence version 2.
DT 03-AUG-2022, entry version 118.
DE RecName: Full=Protein BTG4;
DE AltName: Full=BTG family member 4;
DE AltName: Full=Protein PC3b;
GN Name=Btg4; Synonyms=Pc3b;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Embryo;
RX PubMed=10995567; DOI=10.1006/geno.2000.6288;
RA Buanne P., Corrente G., Micheli L., Palena A., Lavia P., Spadafora C.,
RA Lakshmana M.K., Rinaldi A., Banfi S., Quarto M., Bulfone A., Tirone F.;
RT "Cloning of PC3B, a novel member of the PC3/BTG/TOB family of growth
RT inhibitory genes, highly expressed in the olfactory epithelium.";
RL Genomics 68:253-263(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Mano H.;
RT "The initiate factors from mouse oocyte.";
RL Submitted (NOV-2000) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Egg, and Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [5]
RP FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, INTERACTION WITH CNOT7;
RP CNOT8 AND EIF4E, AND MUTAGENESIS OF HIS-45; TRP-46; PRO-51; ARG-58; CYS-59;
RP ASP-69; TRP-95; GLU-100 AND GLU-107.
RX PubMed=27065194; DOI=10.1038/nsmb.3204;
RA Yu C., Ji S.Y., Sha Q.Q., Dang Y., Zhou J.J., Zhang Y.L., Liu Y.,
RA Wang Z.W., Hu B., Sun Q.Y., Sun S.C., Tang F., Fan H.Y.;
RT "BTG4 is a meiotic cell cycle-coupled maternal-zygotic-transition licensing
RT factor in oocytes.";
RL Nat. Struct. Mol. Biol. 23:387-394(2016).
CC -!- FUNCTION: Adapter protein that bridges CNOT7, a catalytic subunit of
CC the CCR4-NOT complex, to EIF4E, and facilitates maternal mRNAs decay
CC during the maturation of oocytes and in the fertilized egg
CC (PubMed:27065194). It is therefore required for the maternal-zygotic
CC transition (MZT), zygotic cleavage and initiation of embryonic
CC development (PubMed:27065194). {ECO:0000269|PubMed:27065194}.
CC -!- SUBUNIT: Interacts with CNOT7 and EIF4E (PubMed:27065194). Interacts
CC with CNOT8 (PubMed:27065194). {ECO:0000269|PubMed:27065194}.
CC -!- INTERACTION:
CC O70552; Q60809: Cnot7; NbExp=6; IntAct=EBI-16204405, EBI-2104739;
CC O70552; P06730: EIF4E; Xeno; NbExp=4; IntAct=EBI-16204405, EBI-73440;
CC -!- TISSUE SPECIFICITY: Expressed in oocytes (PubMed:27065194). Expressed
CC in testis and in olfactory epithelium. {ECO:0000269|PubMed:27065194}.
CC -!- DISRUPTION PHENOTYPE: BTG4-null mice are viable, have body sizes
CC similar to those of their wild type littermates, are healthy up to 1
CC year of age, and do not spontaneously develop tumors. Females are
CC sterile and their embryos are arrested at the one- or two-cells stage.
CC {ECO:0000269|PubMed:27065194}.
CC -!- SIMILARITY: Belongs to the BTG family. {ECO:0000305}.
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DR EMBL; AJ005120; CAA06384.1; -; mRNA.
DR EMBL; AB050983; BAB47554.2; -; mRNA.
DR EMBL; AK136041; BAE22791.1; -; mRNA.
DR EMBL; AK136149; BAE22846.1; -; mRNA.
DR EMBL; AK161379; BAE36359.1; -; mRNA.
DR EMBL; AC119831; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR CCDS; CCDS40629.1; -.
DR RefSeq; NP_062366.2; NM_019493.4.
DR RefSeq; XP_006510562.1; XM_006510499.3.
DR RefSeq; XP_006510563.1; XM_006510500.2.
DR RefSeq; XP_006510564.1; XM_006510501.3.
DR RefSeq; XP_006510565.1; XM_006510502.2.
DR RefSeq; XP_017168979.1; XM_017313490.1.
DR AlphaFoldDB; O70552; -.
DR SMR; O70552; -.
DR DIP; DIP-61987N; -.
DR IntAct; O70552; 4.
DR STRING; 10090.ENSMUSP00000110074; -.
DR PhosphoSitePlus; O70552; -.
DR PaxDb; O70552; -.
DR PRIDE; O70552; -.
DR ProteomicsDB; 265387; -.
DR Antibodypedia; 32035; 155 antibodies from 23 providers.
DR DNASU; 56057; -.
DR Ensembl; ENSMUST00000114431; ENSMUSP00000110074; ENSMUSG00000032056.
DR Ensembl; ENSMUST00000213680; ENSMUSP00000149181; ENSMUSG00000032056.
DR GeneID; 56057; -.
DR KEGG; mmu:56057; -.
DR UCSC; uc009pld.1; mouse.
DR CTD; 54766; -.
DR MGI; MGI:1860140; Btg4.
DR VEuPathDB; HostDB:ENSMUSG00000032056; -.
DR eggNOG; KOG4006; Eukaryota.
DR GeneTree; ENSGT00950000182952; -.
DR HOGENOM; CLU_079660_3_0_1; -.
DR InParanoid; O70552; -.
DR OrthoDB; 1455216at2759; -.
DR PhylomeDB; O70552; -.
DR TreeFam; TF105272; -.
DR BioGRID-ORCS; 56057; 1 hit in 57 CRISPR screens.
DR ChiTaRS; Btg4; mouse.
DR PRO; PR:O70552; -.
DR Proteomes; UP000000589; Chromosome 9.
DR RNAct; O70552; protein.
DR Bgee; ENSMUSG00000032056; Expressed in primary oocyte and 60 other tissues.
DR ExpressionAtlas; O70552; baseline and differential.
DR Genevisible; O70552; MM.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0003730; F:mRNA 3'-UTR binding; IDA:MGI.
DR GO; GO:0160021; P:maternal-to-zygotic transition of gene expression; IMP:MGI.
DR GO; GO:0008285; P:negative regulation of cell population proliferation; IBA:GO_Central.
DR GO; GO:0045930; P:negative regulation of mitotic cell cycle; IDA:MGI.
DR Gene3D; 3.90.640.90; -; 1.
DR InterPro; IPR002087; Anti_prolifrtn.
DR InterPro; IPR033332; BTG.
DR InterPro; IPR036054; BTG-like_sf.
DR PANTHER; PTHR22978; PTHR22978; 1.
DR Pfam; PF07742; BTG; 1.
DR PRINTS; PR00310; ANTIPRLFBTG1.
DR SMART; SM00099; btg1; 1.
DR SUPFAM; SSF160696; SSF160696; 1.
DR PROSITE; PS00960; BTG_1; 1.
DR PROSITE; PS01203; BTG_2; 1.
PE 1: Evidence at protein level;
KW Reference proteome.
FT CHAIN 1..250
FT /note="Protein BTG4"
FT /id="PRO_0000143811"
FT MUTAGEN 45
FT /note="H->A: Loss of interaction with CNOT7."
FT /evidence="ECO:0000269|PubMed:27065194"
FT MUTAGEN 46
FT /note="W->A: Loss of interaction with CNOT7."
FT /evidence="ECO:0000269|PubMed:27065194"
FT MUTAGEN 51
FT /note="P->A: No effect on interaction with CNOT7."
FT /evidence="ECO:0000269|PubMed:27065194"
FT MUTAGEN 58
FT /note="R->A: Loss of interaction with CNOT7."
FT /evidence="ECO:0000269|PubMed:27065194"
FT MUTAGEN 59
FT /note="C->A: Loss of interaction with CNOT7."
FT /evidence="ECO:0000269|PubMed:27065194"
FT MUTAGEN 69
FT /note="D->A: Loss of interaction with CNOT7."
FT /evidence="ECO:0000269|PubMed:27065194"
FT MUTAGEN 95
FT /note="W->A: Loss of interaction with CNOT7 and CNOT8. No
FT effect on interaction with EIF4E."
FT /evidence="ECO:0000269|PubMed:27065194"
FT MUTAGEN 100
FT /note="E->A: No effect on interaction with CNOT7."
FT /evidence="ECO:0000269|PubMed:27065194"
FT MUTAGEN 107
FT /note="E->A: Loss of interaction with CNOT7."
FT /evidence="ECO:0000269|PubMed:27065194"
FT CONFLICT 37
FT /note="I -> V (in Ref. 1; CAA06384)"
FT /evidence="ECO:0000305"
FT CONFLICT 158
FT /note="I -> V (in Ref. 1; CAA06384)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 250 AA; 28613 MW; 9C175309C017F7BD CRC64;
MRDEIATAVF FVTRLVKKHE KLSTQQIETF ALKLMTILFE KYRGHWHPDC PSKGQAFRCI
RINNNENKDP VLERACAESN VNFFHLGLPK EMTIWVDPYE VCCRYGEKKH PFTIASFKGR
WENWELAQHV SCAVNRATGD CSSGTSSDEE SCSREAQIIP KVNNPKSVYQ VENFKQSLQP
WFCLPRRKHL ADGRGFLPGA ACHPVPKSSK WCRPASRRVD RYHWVNAQLF SGQTAPGEPG
EEALSSLKQK
