ID   TNR0_ECOLX              Reviewed;         194 AA.
AC   P05823;
DT   01-NOV-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1988, sequence version 1.
DT   25-MAY-2022, entry version 84.
DE   RecName: Full=Transposon Tn2501 resolvase;
GN   Name=tnpR;
OS   Escherichia coli.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3027041; DOI=10.1128/jb.169.2.624-631.1987;
RA   Michiels T., Cornelis G., Ellis K., Grinsted J.;
RT   "Tn2501, a component of the lactose transposon Tn951, is an example of a
RT   new category of class II transposable elements.";
RL   J. Bacteriol. 169:624-631(1987).
CC   -!- FUNCTION: Resolvase catalyzes the resolution (a site-specific
CC       recombination) of the cointegrated replicon to yield the final
CC       transposition products.
CC   -!- MISCELLANEOUS: Tn2501 is a cryptic class II transposon found as part of
CC       the lactose transposon Tn951.
CC   -!- SIMILARITY: Belongs to the site-specific recombinase resolvase family.
CC       {ECO:0000305}.
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DR   EMBL; M15197; AAA27426.1; -; Genomic_DNA.
DR   PIR; B27758; RPECR5.
DR   AlphaFoldDB; P05823; -.
DR   SMR; P05823; -.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000150; F:DNA strand exchange activity; IEA:InterPro.
DR   GO; GO:0015074; P:DNA integration; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.1390; -; 1.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR006118; Recombinase_CS.
DR   InterPro; IPR006119; Resolv_N.
DR   InterPro; IPR036162; Resolvase-like_N_sf.
DR   InterPro; IPR006120; Resolvase_HTH_dom.
DR   Pfam; PF02796; HTH_7; 1.
DR   Pfam; PF00239; Resolvase; 1.
DR   SMART; SM00857; Resolvase; 1.
DR   SUPFAM; SSF46689; SSF46689; 1.
DR   SUPFAM; SSF53041; SSF53041; 1.
DR   PROSITE; PS00397; RECOMBINASES_1; 1.
DR   PROSITE; PS00398; RECOMBINASES_2; 1.
DR   PROSITE; PS51736; RECOMBINASES_3; 1.
PE   3: Inferred from homology;
KW   DNA integration; DNA recombination; DNA-binding; Transposable element.
FT   CHAIN           1..194
FT                   /note="Transposon Tn2501 resolvase"
FT                   /id="PRO_0000196377"
FT   DOMAIN          3..143
FT                   /note="Resolvase/invertase-type recombinase catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01072"
FT   DNA_BIND        170..189
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        11
FT                   /note="O-(5'-phospho-DNA)-serine intermediate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01072"
SQ   SEQUENCE   194 AA;  21421 MW;  DEDFC0BA6406EAD1 CRC64;
     MSRVFAYCRV STLEQTTENQ RREIEAAGFA IRPQRLIEEH ISGSVAASER PGFIRLLDRM
     ENGDVLIVTK LDRLGRNAMD IRKTVEQLAS SDIRVHCLAL GGVDLTSAAG RMTMQVISAV
     AEFERDLLLE RTHSGIARAK ATGKRFGRPS ALNEEQQLTV IARINAGISI SAIAREFNTT
     RQTILRVKAG QQSS