ID   TNPT_PSEPU              Reviewed;         181 AA.
AC   P30739;
DT   01-APR-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1993, sequence version 1.
DT   25-MAY-2022, entry version 98.
DE   RecName: Full=Resolvase/recombinase;
OS   Pseudomonas putida (Arthrobacter siderocapsulatus).
OG   Plasmid TOL pWW53, and Plasmid TOL pDK1.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=303;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=HS1, and MT53;
RX   PubMed=1331988; DOI=10.1093/nar/20.20.5476;
RA   Assinder S.J., de Marco P., Sayers J.R., Shaw L.E., Winson M.K.,
RA   Williams P.A.;
RT   "Identical resolvases are encoded by Pseudomonas TOL plasmids pWW53 and
RT   pDK1.";
RL   Nucleic Acids Res. 20:5476-5476(1992).
CC   -!- FUNCTION: Site-specific recombination protein. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the site-specific recombinase resolvase family.
CC       {ECO:0000305}.
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DR   EMBL; L02643; AAA71890.1; -; Unassigned_DNA.
DR   EMBL; L02642; AAA71888.1; -; Unassigned_DNA.
DR   PIR; S35485; S35485.
DR   RefSeq; WP_004574810.1; NZ_MING01000087.1.
DR   RefSeq; YP_003617193.1; NC_014124.1.
DR   RefSeq; YP_709356.1; NC_008275.1.
DR   AlphaFoldDB; P30739; -.
DR   SMR; P30739; -.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000150; F:DNA strand exchange activity; IEA:InterPro.
DR   GO; GO:0015074; P:DNA integration; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.1390; -; 1.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR006118; Recombinase_CS.
DR   InterPro; IPR006119; Resolv_N.
DR   InterPro; IPR036162; Resolvase-like_N_sf.
DR   InterPro; IPR006120; Resolvase_HTH_dom.
DR   Pfam; PF02796; HTH_7; 1.
DR   Pfam; PF00239; Resolvase; 1.
DR   SMART; SM00857; Resolvase; 1.
DR   SUPFAM; SSF46689; SSF46689; 1.
DR   SUPFAM; SSF53041; SSF53041; 1.
DR   PROSITE; PS00397; RECOMBINASES_1; 1.
DR   PROSITE; PS00398; RECOMBINASES_2; 1.
DR   PROSITE; PS51736; RECOMBINASES_3; 1.
PE   3: Inferred from homology;
KW   DNA integration; DNA recombination; DNA-binding; Plasmid.
FT   CHAIN           1..181
FT                   /note="Resolvase/recombinase"
FT                   /id="PRO_0000196379"
FT   DOMAIN          2..137
FT                   /note="Resolvase/invertase-type recombinase catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01072"
FT   DNA_BIND        161..180
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        10
FT                   /note="O-(5'-phospho-DNA)-serine intermediate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01072"
SQ   SEQUENCE   181 AA;  20121 MW;  C84F1DCE7DB3D809 CRC64;
     MRLFGYARVS TSQQSLDVQI KALKAENVRA TRIFTDKVSG SHVNREGLRM LRLKVEEGDV
     VLVKKLDRLG RDTADMIQLI KEFDDMGVAI RFLDDGISTE GTMGKMVVTI LSAVAQAERL
     RILERTNEGR LEAKAKGVKF GRKPTVDKAE VFTLHGQGIS AMEIAKRLKI GRSTVYKVLA
     S