TNPO2_MOUSE
ID TNPO2_MOUSE Reviewed; 887 AA.
AC Q99LG2; Q8C0U9;
DT 15-MAR-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 133.
DE RecName: Full=Transportin-2;
DE AltName: Full=Karyopherin beta-2b;
GN Name=Tnpo2;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 331-887.
RC STRAIN=C57BL/6J; TISSUE=Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas,
RC Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Probably functions in nuclear protein import as nuclear
CC transport receptor. Serves as receptor for nuclear localization signals
CC (NLS) in cargo substrates. Is thought to mediate docking of the
CC importin/substrate complex to the nuclear pore complex (NPC) through
CC binding to nucleoporin and the complex is subsequently translocated
CC through the pore by an energy requiring, Ran-dependent mechanism. At
CC the nucleoplasmic side of the NPC, Ran binds to the importin, the
CC importin/substrate complex dissociates and importin is re-exported from
CC the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The
CC directionality of nuclear import is thought to be conferred by an
CC asymmetric distribution of the GTP- and GDP-bound forms of Ran between
CC the cytoplasm and nucleus (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Nucleus {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the importin beta family. Importin beta-2
CC subfamily. {ECO:0000305}.
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DR EMBL; BC003275; AAH03275.1; -; mRNA.
DR EMBL; AK029774; BAC26611.1; -; mRNA.
DR CCDS; CCDS90431.1; -.
DR RefSeq; NP_001116315.1; NM_001122843.1.
DR RefSeq; NP_663365.3; NM_145390.4.
DR RefSeq; XP_011246644.1; XM_011248342.2.
DR RefSeq; XP_011246647.1; XM_011248345.2.
DR RefSeq; XP_017168136.1; XM_017312647.1.
DR RefSeq; XP_017168137.1; XM_017312648.1.
DR AlphaFoldDB; Q99LG2; -.
DR SMR; Q99LG2; -.
DR BioGRID; 229382; 4.
DR STRING; 10090.ENSMUSP00000133076; -.
DR iPTMnet; Q99LG2; -.
DR PhosphoSitePlus; Q99LG2; -.
DR SwissPalm; Q99LG2; -.
DR EPD; Q99LG2; -.
DR jPOST; Q99LG2; -.
DR MaxQB; Q99LG2; -.
DR PaxDb; Q99LG2; -.
DR PeptideAtlas; Q99LG2; -.
DR PRIDE; Q99LG2; -.
DR ProteomicsDB; 259607; -.
DR Antibodypedia; 26113; 95 antibodies from 20 providers.
DR DNASU; 212999; -.
DR Ensembl; ENSMUST00000211601; ENSMUSP00000147583; ENSMUSG00000031691.
DR GeneID; 212999; -.
DR KEGG; mmu:212999; -.
DR UCSC; uc009mpa.2; mouse.
DR CTD; 30000; -.
DR MGI; MGI:2384849; Tnpo2.
DR VEuPathDB; HostDB:ENSMUSG00000031691; -.
DR eggNOG; KOG2023; Eukaryota.
DR GeneTree; ENSGT00940000156708; -.
DR InParanoid; Q99LG2; -.
DR OrthoDB; 525700at2759; -.
DR PhylomeDB; Q99LG2; -.
DR BioGRID-ORCS; 212999; 5 hits in 74 CRISPR screens.
DR ChiTaRS; Tnpo2; mouse.
DR PRO; PR:Q99LG2; -.
DR Proteomes; UP000000589; Chromosome 8.
DR RNAct; Q99LG2; protein.
DR Bgee; ENSMUSG00000031691; Expressed in perirhinal cortex and 221 other tissues.
DR ExpressionAtlas; Q99LG2; baseline and differential.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0061608; F:nuclear import signal receptor activity; IBA:GO_Central.
DR GO; GO:0008139; F:nuclear localization sequence binding; IBA:GO_Central.
DR GO; GO:0031267; F:small GTPase binding; IEA:InterPro.
DR GO; GO:0051148; P:negative regulation of muscle cell differentiation; IMP:MGI.
DR GO; GO:0006606; P:protein import into nucleus; IMP:MGI.
DR Gene3D; 1.25.10.10; -; 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR001494; Importin-beta_N.
DR InterPro; IPR040122; Importin_beta.
DR PANTHER; PTHR10527; PTHR10527; 1.
DR Pfam; PF03810; IBN_N; 1.
DR SMART; SM00913; IBN_N; 1.
DR SUPFAM; SSF48371; SSF48371; 1.
PE 1: Evidence at protein level;
KW Acetylation; Cytoplasm; Nucleus; Protein transport; Reference proteome;
KW Repeat; Transport.
FT CHAIN 1..887
FT /note="Transportin-2"
FT /id="PRO_0000120768"
FT REPEAT 9..36
FT /note="HEAT 1"
FT /evidence="ECO:0000250|UniProtKB:Q92973"
FT DOMAIN 31..99
FT /note="Importin N-terminal"
FT REPEAT 41..79
FT /note="HEAT 2"
FT /evidence="ECO:0000250|UniProtKB:Q92973"
FT REPEAT 88..121
FT /note="HEAT 3"
FT /evidence="ECO:0000250|UniProtKB:Q92973"
FT REPEAT 127..164
FT /note="HEAT 4"
FT /evidence="ECO:0000250|UniProtKB:Q92973"
FT REPEAT 171..201
FT /note="HEAT 5"
FT /evidence="ECO:0000250|UniProtKB:Q92973"
FT REPEAT 214..241
FT /note="HEAT 6"
FT /evidence="ECO:0000250|UniProtKB:Q92973"
FT REPEAT 253..280
FT /note="HEAT 7"
FT /evidence="ECO:0000250|UniProtKB:Q92973"
FT REPEAT 296..386
FT /note="HEAT 8"
FT /evidence="ECO:0000250|UniProtKB:Q92973"
FT REPEAT 394..422
FT /note="HEAT 9"
FT /evidence="ECO:0000250|UniProtKB:Q92973"
FT REPEAT 434..461
FT /note="HEAT 10"
FT /evidence="ECO:0000250|UniProtKB:Q92973"
FT REPEAT 475..508
FT /note="HEAT 11"
FT /evidence="ECO:0000250|UniProtKB:Q92973"
FT REPEAT 516..549
FT /note="HEAT 12"
FT /evidence="ECO:0000250|UniProtKB:Q92973"
FT REPEAT 557..595
FT /note="HEAT 13"
FT /evidence="ECO:0000250|UniProtKB:Q92973"
FT REPEAT 603..654
FT /note="HEAT 14"
FT /evidence="ECO:0000250|UniProtKB:Q92973"
FT REPEAT 665..696
FT /note="HEAT 15"
FT /evidence="ECO:0000250|UniProtKB:Q92973"
FT REPEAT 704..737
FT /note="HEAT 16"
FT /evidence="ECO:0000250|UniProtKB:Q92973"
FT REPEAT 745..780
FT /note="HEAT 17"
FT /evidence="ECO:0000250|UniProtKB:Q92973"
FT REPEAT 788..821
FT /note="HEAT 18"
FT /evidence="ECO:0000250|UniProtKB:Q92973"
FT REPEAT 830..861
FT /note="HEAT 19"
FT /evidence="ECO:0000250|UniProtKB:Q92973"
FT REPEAT 864..884
FT /note="HEAT 20"
FT /evidence="ECO:0000250|UniProtKB:Q92973"
FT REGION 344..363
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 852
FT /note="N6-acetyllysine"
FT /evidence="ECO:0000250|UniProtKB:O14787"
FT CONFLICT 361
FT /note="D -> G (in Ref. 2; BAC26611)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 887 AA; 100456 MW; 0A633DCE4DAC4E93 CRC64;
MDWQPDEQGL QQVLQLLKDS QSPNTATQRI VQDKLKQLNQ FPDFNNYLIF VLTRLKSEDE
PTRSLSGLIL KNNVKAHYQS FPPPVADFIK QECLNNIGDA SSLIRATIGI LITTIASKGE
LQMWPELLPQ LCNLLNSEDY NTCEGAFGAL QKICEDSSEL LDSDALNRPL NIMIPKFLQF
FKHCSPKIRS HAIACVNQFI MDRAQALMDN IDTFIEHLFA LAVDDDPEVR KNVCRALVML
LEVRIDRLIP HMHSIIQYML QRTQDHDENV ALEACEFWLT LAEQPICKEV LASHLVQLIP
ILVNGMKYSE IDIILLKGDV EEDEAVPDSE QDIKPRFHKS RTVTLTHEAE RPDSSEDAED
DDDDDALSDW NLRKCSAAAL DVLANVFREE LLPHLLPLLK GLLFHPEWVV KESGILVLGA
IAEGCMQGMV PYLPELIPHL IQCLSDKKAL VRSIACWTLS RYAHWVVSQP PDMHLKPLMT
ELLKRILDGN KRVQEAACSA FATLEEEACT ELVPYLSYIL DTLVFAFGKY QHKNLLILYD
AIGTLADSVG HHLNQPEYIQ KLMPPLIQKW NELKDEDKDL FPLLECLSSV ATALQSGFLP
YCEPVYQRCV TLVQKTLAQA MMYTQHPEQY EAPDKDFMIV ALDLLSGLAE GLGGHVEQLV
ARSNIMTLLF QCMQDSMPEV RQSSFALLGD LTKACFIHVK PCIAEFMPIL GTNLNPEFIS
VCNNATWAIG EICMQMGAEM QPYVQMVLNN LVEIINRPNT PKTLLENTAI TIGRLGYVCP
QEVAPMLQQF IRPWCTSLRN IRDNEEKDSA FRGICMMIGV NPGGVVQDFI FFCDAVASWV
SPKDDLRDMF YKILHGFKDQ VGEENWQQFS EQFPPLLKER LAAFYGV
