ID   TNPE_STAAU              Reviewed;         630 AA.
AC   P37375;
DT   01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1994, sequence version 1.
DT   25-MAY-2022, entry version 74.
DE   RecName: Full=Transposase B from transposon PsiTn554;
GN   Name=tnpB;
OS   Staphylococcus aureus.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=1280;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   TRANSPOSON=PsiTn554;
RA   Chikramane S.G., Dubin D.T.;
RT   "PsiTn554: a Staphylococcus aureus chromosomal element encoding cadmium
RT   resistance determinants, and genes resembling the transposases genes of
RT   Tn554.";
RL   Submitted (FEB-1993) to the EMBL/GenBank/DDBJ databases.
CC   -!- SIMILARITY: Belongs to the 'phage' integrase family. {ECO:0000305}.
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DR   EMBL; L10909; AAA26607.1; -; Genomic_DNA.
DR   AlphaFoldDB; P37375; -.
DR   SMR; P37375; -.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0015074; P:DNA integration; IEA:UniProtKB-KW.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.150.130; -; 1.
DR   Gene3D; 1.10.443.10; -; 1.
DR   InterPro; IPR044068; CB.
DR   InterPro; IPR011010; DNA_brk_join_enz.
DR   InterPro; IPR013762; Integrase-like_cat_sf.
DR   InterPro; IPR002104; Integrase_catalytic.
DR   InterPro; IPR010998; Integrase_recombinase_N.
DR   Pfam; PF00589; Phage_integrase; 1.
DR   SUPFAM; SSF56349; SSF56349; 1.
DR   PROSITE; PS51900; CB; 1.
DR   PROSITE; PS51898; TYR_RECOMBINASE; 1.
PE   3: Inferred from homology;
KW   DNA integration; DNA recombination; DNA-binding; Transposable element.
FT   CHAIN           1..630
FT                   /note="Transposase B from transposon PsiTn554"
FT                   /id="PRO_0000197556"
FT   DOMAIN          216..302
FT                   /note="Core-binding (CB)"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01248"
FT   DOMAIN          326..513
FT                   /note="Tyr recombinase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        363
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        391
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        465
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        468
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        491
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        500
FT                   /note="O-(3'-phospho-DNA)-tyrosine intermediate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
SQ   SEQUENCE   630 AA;  74417 MW;  66BAD686B713FF55 CRC64;
     MNASSKRKII SQSEISKKIA VMNEEMQGFW ANNSWDIRKC PHPSAIELSK NPALRNRWVR
     FERVKNLWLR TELKYFYFYH LNNGIWNAKT VWIRKGTVIN KMLDFLDLKY PSITSITEVP
     IDKAMTEYRT YLTKQGVRIT TTNYKITANQ EKTPVKANSY YVTNLKQFIE FIEDFYFDGE
     EWDKDVWDRR NLPLPDDKVN PTQYEYTINF KGFRNTYFKQ LVKRYCKLRL NMDSFSYVSD
     IAQKLKEFFN FLDIKFKHVQ RVHQLTRVEI EAYLSELNMM GIKPSTITGR ISILEGLFST
     LHRLEWDDVP SKLLIYPEDY PKIPRAKPRF IDEFVLEQLN SHLDKLPEYI ATMTMIVQEC
     GMRISELCTL KKGCLLEDKD GYYFLKYYQW KMKKEHIVPI SKEVVLLIKV REDKVSEEFP
     DSEYLFPRKD GSPLKQETFR GELNKLAYEQ NIVDKLGEIY RFHAHAFRHS VGTRTINNGV
     PQHIVQKFLG HESPEMTSRY AHIFDETLKN EFTKFQEKLV TNNGDVLNLD DDSEVDDVEL
     QWFKKNINAQ VLPNGYCRLP VIAGGCPHAN ACLDCTHFCT SKQFLPQHEE QLERTEELLT
     IAKDKQWQRQ IETNSRVKER LEQIIGSLTG