ID   TNPB_STAAU              Reviewed;         630 AA.
AC   P0A055; P06697;
DT   01-JAN-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1988, sequence version 1.
DT   25-MAY-2022, entry version 72.
DE   RecName: Full=Transposase B from transposon Tn554;
GN   Name=tnpB;
OS   Staphylococcus aureus.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=1280;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3004956; DOI=10.1002/j.1460-2075.1985.tb04089.x;
RA   Murphy E., Huwyler L., Do Carno de Freire Bastos M.;
RT   "Transposon Tn554: complete nucleotide sequence and isolation of
RT   transposition-defective and antibiotic-sensitive mutants.";
RL   EMBO J. 4:3357-3365(1985).
CC   -!- FUNCTION: One of three proteins encoded by transposon Tn554 required
CC       for its transposition.
CC   -!- SIMILARITY: Belongs to the 'phage' integrase family. {ECO:0000305}.
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DR   EMBL; X03216; CAA26961.1; -; Genomic_DNA.
DR   PIR; B24584; B24584.
DR   RefSeq; WP_001557544.1; NZ_WWCF01000004.1.
DR   AlphaFoldDB; P0A055; -.
DR   SMR; P0A055; -.
DR   GeneID; 42043716; -.
DR   GeneID; 58051000; -.
DR   OMA; LNSERMI; -.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0015074; P:DNA integration; IEA:UniProtKB-KW.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.150.130; -; 1.
DR   Gene3D; 1.10.443.10; -; 1.
DR   InterPro; IPR044068; CB.
DR   InterPro; IPR011010; DNA_brk_join_enz.
DR   InterPro; IPR013762; Integrase-like_cat_sf.
DR   InterPro; IPR002104; Integrase_catalytic.
DR   InterPro; IPR010998; Integrase_recombinase_N.
DR   Pfam; PF00589; Phage_integrase; 1.
DR   SUPFAM; SSF56349; SSF56349; 1.
DR   PROSITE; PS51900; CB; 1.
DR   PROSITE; PS51898; TYR_RECOMBINASE; 1.
PE   3: Inferred from homology;
KW   DNA integration; DNA recombination; DNA-binding; Transposable element.
FT   CHAIN           1..630
FT                   /note="Transposase B from transposon Tn554"
FT                   /id="PRO_0000197555"
FT   DOMAIN          216..302
FT                   /note="Core-binding (CB)"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01248"
FT   DOMAIN          326..513
FT                   /note="Tyr recombinase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        363
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        391
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        465
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        468
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        491
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        500
FT                   /note="O-(3'-phospho-DNA)-tyrosine intermediate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
SQ   SEQUENCE   630 AA;  74439 MW;  75EB856058DC6319 CRC64;
     MNASSKRKII SQSEISKKIA VMNEEMQGFW ANNSWDIRKC PHPSAIELSK NPALRNRWVR
     FERVKNLWLR TELKYFYFYH LNNGIWNAKT VWIRKGTVIN KMLDFLDLKY PSITSITEVP
     IEKAMTEYRT YLTKRGVRIT TTNYKITANQ EKTPVKANSY YVTNLKQFME FYENFYFDGE
     EWDKDVWDRR NLPLPDDKVN PTQYEYTINF KGFRNTYFKQ LVKRYCKLRL NVDSFSYVSD
     IAQRLKEFFN FLDMKFKQVQ RVHQLTRVEI EAYLSELNMM GIKPSTITGR ISILEGLFST
     LLRLEWDDVP SKILIYSEDY PKIPRAKPRF IDEFVLEQLN SHLDKLPEYI ATMTMIVQEC
     GMRISELCTL KKGCLLEDKD GDFFLKYYQW KMKKEHIVPI SKEVALLIKV REDKVSEEFP
     DSEYLFPRKD GSPLKQETFR GELNKLAYEQ NIVDKSGEIY RFHAHAFRHT VGTRMINNGM
     PQHIVQKFLG HESPEMTSRY AHIFDETLKN EFTKFQEKLV TNNGDVLDLD EDNEVDDVEL
     QWFKKNINAQ VLPNGYCRLP VVAGGCPHAN ACLDCTHFCT SKQFLPQHEE QLERTEELLA
     IAKDKQWQRQ VETNSRVKER LEQIIGSLTG