ID   TNPA_STAAU              Reviewed;         361 AA.
AC   P0A052; P06696;
DT   01-JAN-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1988, sequence version 1.
DT   25-MAY-2022, entry version 74.
DE   RecName: Full=Transposase A from transposon Tn554;
GN   Name=tnpA;
OS   Staphylococcus aureus.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=1280;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3004956; DOI=10.1002/j.1460-2075.1985.tb04089.x;
RA   Murphy E., Huwyler L., Do Carno de Freire Bastos M.;
RT   "Transposon Tn554: complete nucleotide sequence and isolation of
RT   transposition-defective and antibiotic-sensitive mutants.";
RL   EMBO J. 4:3357-3365(1985).
CC   -!- FUNCTION: One of three proteins encoded by transposon Tn554 required
CC       for its transposition.
CC   -!- SIMILARITY: Belongs to the 'phage' integrase family. {ECO:0000305}.
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DR   EMBL; X03216; CAA26960.1; -; Genomic_DNA.
DR   PIR; A24584; A24584.
DR   RefSeq; WP_000868132.1; NZ_WHRH01000083.1.
DR   AlphaFoldDB; P0A052; -.
DR   SMR; P0A052; -.
DR   GeneID; 42043715; -.
DR   GeneID; 58050999; -.
DR   OMA; YHTEEVE; -.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0015074; P:DNA integration; IEA:UniProtKB-KW.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR   CDD; cd01186; INT_tnpA_C_Tn554; 1.
DR   Gene3D; 1.10.150.130; -; 1.
DR   Gene3D; 1.10.443.10; -; 1.
DR   InterPro; IPR044068; CB.
DR   InterPro; IPR011010; DNA_brk_join_enz.
DR   InterPro; IPR042721; INT_tnpA_C_Tn554.
DR   InterPro; IPR013762; Integrase-like_cat_sf.
DR   InterPro; IPR002104; Integrase_catalytic.
DR   InterPro; IPR010998; Integrase_recombinase_N.
DR   InterPro; IPR004107; Integrase_SAM-like_N.
DR   Pfam; PF02899; Phage_int_SAM_1; 1.
DR   Pfam; PF00589; Phage_integrase; 1.
DR   SUPFAM; SSF56349; SSF56349; 1.
DR   PROSITE; PS51900; CB; 1.
DR   PROSITE; PS51898; TYR_RECOMBINASE; 1.
PE   3: Inferred from homology;
KW   DNA integration; DNA recombination; DNA-binding; Transposable element.
FT   CHAIN           1..361
FT                   /note="Transposase A from transposon Tn554"
FT                   /id="PRO_0000197552"
FT   DOMAIN          23..120
FT                   /note="Core-binding (CB)"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01248"
FT   DOMAIN          163..351
FT                   /note="Tyr recombinase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        198
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        232
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        302
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        305
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        328
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        338
FT                   /note="O-(3'-phospho-DNA)-tyrosine intermediate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
SQ   SEQUENCE   361 AA;  42941 MW;  44ACFA4A7216913B CRC64;
     MKVQRIEVEN KPYPLYLLLD KEYQLIEPVM KFIKYLDNTG KSPNTIKAYC YHLKLLYEFM
     EQRGVILNDI NFELLADFVG WLRYPSASNV IDLQSKKAIR EETTVNTILN VVMSFLDYLS
     RLGEFKSIDV FKQAKGRNFK GFLHHVNKGR YQKNVLKLRV KKKQIRTLRS KEVKQIIDAC
     HTKRDKLILM LMYEGGLRIG EVLSLRLEDI VTWDNQIHLT PRDVNVNEAY IKLRKERTIH
     VSKELMSLYT DYLIYEYSEE LEHDYVFISL KEGYFGKPLK YQSVLDLVRR IVKRTGIEFT
     SHMLRHTHAT QLIREGWDVA FVQKRLGHAH VQTTLNTYVH LSDQDMKNEF NKYLERKEHK
     K