ID   TNPA_ECOLI              Reviewed;        1002 AA.
AC   Q00037;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   25-MAY-2022, entry version 87.
DE   RecName: Full=Transposase for transposon gamma-delta;
DE   AltName: Full=Transposon Tn1000 transposase;
GN   Name=tnpA; OrderedLocusNames=ECOK12F010;
OS   Escherichia coli (strain K12).
OG   Plasmid F.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=7612932; DOI=10.3109/10425179509029361;
RA   Broom J.E., Hill D.F., Hughes G., Jones W.A., McNaughton J.C.,
RA   Stockwell P.A., Petersen G.B.;
RT   "Sequence of a transposon identified as Tn1000 (gamma delta).";
RL   DNA Seq. 5:185-189(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8080658; DOI=10.1266/jjg.69.269;
RA   Maekawa T., Ohtsubo E.;
RT   "Identification of the region that determines the specificity of binding of
RT   the transposases encoded by Tn3 and gamma delta to the terminal inverted
RT   repeat sequences.";
RL   Jpn. J. Genet. 69:269-285(1994).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / CR63; PLASMID=F;
RA   Shimizu H., Saitoh Y., Suda Y., Uehara K., Sampei G., Mizobuchi K.;
RT   "Complete nucleotide sequence of the F plasmid: its implications for
RT   organization and diversification of plasmid genomes.";
RL   Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 811-1002.
RC   STRAIN=K12 / HFRC;
RX   PubMed=1709493; DOI=10.1093/nar/19.8.1845;
RA   Albrechtsen B., Ross B.M., Squires C., Squires C.L.;
RT   "Transcriptional termination sequence at the end of the Escherichia coli
RT   ribosomal RNA G operon: complex terminators and antitermination.";
RL   Nucleic Acids Res. 19:1845-1852(1991).
CC   -!- FUNCTION: Required for transposition of transposon Tn1000.
CC   -!- SIMILARITY: Belongs to the transposase 7 family. {ECO:0000305}.
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DR   EMBL; X60200; CAA42760.1; -; Genomic_DNA.
DR   EMBL; D16449; BAA03914.1; -; Genomic_DNA.
DR   EMBL; X56780; CAA40100.1; -; Genomic_DNA.
DR   EMBL; AP001918; BAA97880.1; -; Genomic_DNA.
DR   PIR; I56963; I56963.
DR   RefSeq; NP_061389.1; NC_002483.1.
DR   RefSeq; WP_001143750.1; NZ_CP047127.1.
DR   AlphaFoldDB; Q00037; -.
DR   PRIDE; Q00037; -.
DR   PATRIC; fig|83333.107.peg.593; -.
DR   PRO; PR:Q00037; -.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0004803; F:transposase activity; IEA:InterPro.
DR   GO; GO:0006313; P:transposition, DNA-mediated; IEA:InterPro.
DR   InterPro; IPR025296; DUF4158.
DR   InterPro; IPR002513; Tn3_Tnp_DDE_dom.
DR   Pfam; PF01526; DDE_Tnp_Tn3; 1.
DR   Pfam; PF13700; DUF4158; 1.
PE   3: Inferred from homology;
KW   DNA recombination; DNA-binding; Plasmid; Transposable element;
KW   Transposition.
FT   CHAIN           1..1002
FT                   /note="Transposase for transposon gamma-delta"
FT                   /id="PRO_0000075426"
SQ   SEQUENCE   1002 AA;  113788 MW;  3278F27D2BEA15AC CRC64;
     MPVDFLTSDQ KQNYGCYAAE PNDVQLARYF HLDERDLAFI NQRRGKHNRL GIALQLTTAR
     FLGTFLTDLT QVLPGVQHFV AVQLNIHRPE VLSRYAERDT TLREHTALIK EYYGYHEFGD
     FPWSFRLKRL LYTRAWLSNE RPGLMFDFAT AWLLQNKVLL PGATTLVRLI SEIRERANQR
     LWKKLAALPN KWQAAQVMEL LVIPEGQRVS ALEQLKKGPV TVSGPAFNEA LERYIRLRSL
     EFSRLNFSGL PAIQLRNLAR YAGMASVKYI ARMPQQRKLA VLTAFVKAQE ITALDDAVDV
     LDMLILDIIR EAKKTGQKKR LRTLKDLDQA ALLLARACAL LLDDNTDVPD LRQVIFKCVP
     KNRLAESVSK VNELARPQNN NFHDEMVEQY GRVKRFLPAV LRDLHFRAAP AGEHVLAAIH
     YLAELNGSKK RILDDAPEHI ITGPWKRLVY DAEGRIQRAG YSLCLLERLQ DALRRRDIWL
     ENSDRWGDPR EKLLQGEEWQ TQRIPVCRAL GHPVDGRKGV QQLAIQLDET WKAVASRFEK
     NAEVHICNEG KYPSLTISCL EKQEEPPSLL RLNNRIKQLL PPVDLTELLL EIDAQTGFTH
     EFAHVSESGA RAQDLHISLC AVLMAEACNI GLEPLIKHNI PALTRHRLSW VKQNYLRAET
     LVSANARLVD FQSTLELAGR WGGGEVASAD GMRFVTPVKT INSGSNRKYF GSGRGITWYN
     FVSDQYSGFH GIVVPGTLRD SIFVLEGLLE QQTGLNPVEI MTDTAGSSDI IFGLFWLLGY
     QFSPRLADAG EAVFWRVNKS ANYGVLDKLA RGYADLSKAE SQWDEMMRTA GSLKLGTIHA
     SELIRSLLKS SRPSGLAQAI MEVGRVNKTL YLLNYIDDED YRRRILTQLN RGEGRHAVAR
     AICYGQRGEI RKRYREGQED QLGALGLVTN AVVLWNTLYM EEALSWMRRN GEEIIDEDIA
     RLSPLMHGHI NMLGHYTFTL PEDILKGELR ALNLNINNEL SP