TNNT3_MOUSE
ID TNNT3_MOUSE Reviewed; 272 AA.
AC Q9QZ47; A2A6H7; A2A6H8; A2A6H9; A2A6I1; A2A6I2; A2A6I4; A2A6I6; A2A6I7;
AC A2A6I9; O35575; O35576; O35577; O35578; O35579; O35580; O35581; O35582;
AC O35583; O35584; O35585; P97456; Q99L89; Q9QZ46;
DT 04-AUG-2003, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 3.
DT 03-AUG-2022, entry version 142.
DE RecName: Full=Troponin T, fast skeletal muscle;
DE Short=TnTf;
DE AltName: Full=Fast skeletal muscle troponin T;
DE Short=fTnT;
GN Name=Tnnt3;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], ALTERNATIVE SPLICING, TISSUE SPECIFICITY, AND
RP DEVELOPMENTAL STAGE.
RC STRAIN=129/SvJ; TISSUE=Skeletal muscle;
RX PubMed=9249073; DOI=10.1016/s0378-1119(97)00100-5;
RA Wang J., Jin J.-P.;
RT "Primary structure and developmental acidic to basic transition of 13
RT alternatively spliced mouse fast skeletal muscle troponin T isoforms.";
RL Gene 193:105-114(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B3E17).
RX PubMed=9107680; DOI=10.1007/s003359900437;
RA Koch A., Juan T.S.-C., Jenkins N.A., Gilbert D.J., Copeland N.G.,
RA McNiece I.K., Fletcher F.A.;
RT "cDNA cloning and chromosomal mapping of mouse fast skeletal muscle
RT troponin T.";
RL Mamm. Genome 8:346-348(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND VARIANT ARG-131.
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 14).
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-162, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brown adipose tissue;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Troponin T is the tropomyosin-binding subunit of troponin,
CC the thin filament regulatory complex which confers calcium-sensitivity
CC to striated muscle actomyosin ATPase activity. {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=14;
CC Comment=Additional isoforms seem to exist. Isoforms A1e17, A2e17,
CC A3e17, A4e17, A5e17 and A6e17 are acidic while isoforms B1e16, B2e17,
CC B2e16, B3e17, B3e16, B4e17 and B4e16 are basic.;
CC Name=A1e17;
CC IsoId=Q9QZ47-1; Sequence=Displayed;
CC Name=A2e17;
CC IsoId=Q9QZ47-2; Sequence=VSP_050341;
CC Name=A3e17;
CC IsoId=Q9QZ47-3; Sequence=VSP_050339;
CC Name=A4e17;
CC IsoId=Q9QZ47-4; Sequence=VSP_050338, VSP_050341;
CC Name=A5e17;
CC IsoId=Q9QZ47-5; Sequence=VSP_050343;
CC Name=A6e17;
CC IsoId=Q9QZ47-6; Sequence=VSP_050341, VSP_050343;
CC Name=B1e16;
CC IsoId=Q9QZ47-7; Sequence=VSP_050342, VSP_050344;
CC Name=B2e17;
CC IsoId=Q9QZ47-8; Sequence=VSP_050339, VSP_050343;
CC Name=B2e16;
CC IsoId=Q9QZ47-9; Sequence=VSP_050339, VSP_050343, VSP_050344;
CC Name=B3e17; Synonyms=PTROPT;
CC IsoId=Q9QZ47-10; Sequence=VSP_050338, VSP_050342;
CC Name=B3e16;
CC IsoId=Q9QZ47-11; Sequence=VSP_050338, VSP_050342, VSP_050344;
CC Name=B4e17;
CC IsoId=Q9QZ47-12; Sequence=VSP_050338, VSP_050340;
CC Name=B4e16;
CC IsoId=Q9QZ47-13; Sequence=VSP_050338, VSP_050340, VSP_050344;
CC Name=14;
CC IsoId=Q9QZ47-14; Sequence=VSP_050344;
CC -!- TISSUE SPECIFICITY: Expressed predominantly in skeletal muscle.
CC {ECO:0000269|PubMed:9249073}.
CC -!- DEVELOPMENTAL STAGE: Embryo and neonate express predominantly acidic
CC isoforms while all adult isoforms are basic. Only one isoform, B2e17,
CC is found in both embryo/neonate and adult. A transition from high to
CC low molecular weight isoforms is also seen during postnatal
CC development. {ECO:0000269|PubMed:9249073}.
CC -!- SIMILARITY: Belongs to the troponin T family. {ECO:0000305}.
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DR EMBL; L49466; AAB67290.1; -; mRNA.
DR EMBL; L49467; AAF01502.1; -; mRNA.
DR EMBL; L49468; AAF01503.1; -; mRNA.
DR EMBL; L49470; AAB67291.1; -; mRNA.
DR EMBL; L49471; AAB67292.1; -; mRNA.
DR EMBL; L49472; AAB67293.1; -; mRNA.
DR EMBL; L48988; AAB67283.1; -; mRNA.
DR EMBL; L48989; AAB67284.1; -; mRNA.
DR EMBL; L48990; AAB67285.1; -; mRNA.
DR EMBL; L48991; AAB67286.1; -; mRNA.
DR EMBL; L48992; AAB67287.1; -; mRNA.
DR EMBL; L48993; AAB67288.1; -; mRNA.
DR EMBL; L49018; AAB67289.1; -; mRNA.
DR EMBL; U77779; AAB39743.1; -; mRNA.
DR EMBL; AL603651; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC003747; AAH03747.1; -; mRNA.
DR CCDS; CCDS22031.1; -. [Q9QZ47-10]
DR CCDS; CCDS52451.1; -. [Q9QZ47-14]
DR CCDS; CCDS52452.1; -. [Q9QZ47-1]
DR CCDS; CCDS52453.1; -. [Q9QZ47-5]
DR CCDS; CCDS52454.1; -. [Q9QZ47-2]
DR CCDS; CCDS52455.1; -. [Q9QZ47-6]
DR CCDS; CCDS52456.1; -. [Q9QZ47-3]
DR CCDS; CCDS85471.1; -. [Q9QZ47-7]
DR CCDS; CCDS85472.1; -. [Q9QZ47-9]
DR CCDS; CCDS85473.1; -. [Q9QZ47-8]
DR CCDS; CCDS85474.1; -. [Q9QZ47-11]
DR CCDS; CCDS85475.1; -. [Q9QZ47-4]
DR CCDS; CCDS85476.1; -. [Q9QZ47-13]
DR CCDS; CCDS85477.1; -. [Q9QZ47-12]
DR RefSeq; NP_001157137.1; NM_001163665.1.
DR RefSeq; NP_001157140.1; NM_001163668.1.
DR RefSeq; NP_001334476.1; NM_001347547.1.
DR RefSeq; NP_001334477.1; NM_001347548.1.
DR RefSeq; NP_001334478.1; NM_001347549.1.
DR RefSeq; NP_001334479.1; NM_001347550.1.
DR RefSeq; NP_001334480.1; NM_001347551.1.
DR RefSeq; NP_001334481.1; NM_001347552.1.
DR RefSeq; NP_035750.2; NM_011620.3.
DR AlphaFoldDB; Q9QZ47; -.
DR SMR; Q9QZ47; -.
DR BioGRID; 204269; 1.
DR IntAct; Q9QZ47; 1.
DR MINT; Q9QZ47; -.
DR STRING; 10090.ENSMUSP00000136278; -.
DR iPTMnet; Q9QZ47; -.
DR PhosphoSitePlus; Q9QZ47; -.
DR jPOST; Q9QZ47; -.
DR MaxQB; Q9QZ47; -.
DR PaxDb; Q9QZ47; -.
DR PRIDE; Q9QZ47; -.
DR ProteomicsDB; 258796; -. [Q9QZ47-1]
DR ProteomicsDB; 258797; -. [Q9QZ47-2]
DR ProteomicsDB; 258798; -. [Q9QZ47-3]
DR ProteomicsDB; 258799; -. [Q9QZ47-4]
DR ProteomicsDB; 258800; -. [Q9QZ47-5]
DR ProteomicsDB; 258801; -. [Q9QZ47-6]
DR ProteomicsDB; 258802; -. [Q9QZ47-7]
DR ProteomicsDB; 258803; -. [Q9QZ47-8]
DR ProteomicsDB; 258804; -. [Q9QZ47-9]
DR ProteomicsDB; 258805; -. [Q9QZ47-10]
DR ProteomicsDB; 258806; -. [Q9QZ47-11]
DR ProteomicsDB; 258807; -. [Q9QZ47-12]
DR ProteomicsDB; 258808; -. [Q9QZ47-13]
DR ProteomicsDB; 258809; -. [Q9QZ47-14]
DR DNASU; 21957; -.
DR GeneID; 21957; -.
DR KEGG; mmu:21957; -.
DR UCSC; uc009knj.2; mouse. [Q9QZ47-10]
DR UCSC; uc009knp.2; mouse. [Q9QZ47-14]
DR CTD; 7140; -.
DR MGI; MGI:109550; Tnnt3.
DR eggNOG; KOG3634; Eukaryota.
DR InParanoid; Q9QZ47; -.
DR OrthoDB; 1480247at2759; -.
DR PhylomeDB; Q9QZ47; -.
DR TreeFam; TF313321; -.
DR Reactome; R-MMU-390522; Striated Muscle Contraction.
DR BioGRID-ORCS; 21957; 1 hit in 71 CRISPR screens.
DR PRO; PR:Q9QZ47; -.
DR Proteomes; UP000000589; Unplaced.
DR RNAct; Q9QZ47; protein.
DR GO; GO:0005861; C:troponin complex; ISO:MGI.
DR GO; GO:0003779; F:actin binding; ISO:MGI.
DR GO; GO:0048306; F:calcium-dependent protein binding; ISO:MGI.
DR GO; GO:0005523; F:tropomyosin binding; ISO:MGI.
DR GO; GO:0030172; F:troponin C binding; ISO:MGI.
DR GO; GO:0031013; F:troponin I binding; ISO:MGI.
DR GO; GO:0006936; P:muscle contraction; IBA:GO_Central.
DR GO; GO:1903612; P:positive regulation of calcium-dependent ATPase activity; ISO:MGI.
DR GO; GO:0043462; P:regulation of ATP-dependent activity; ISO:MGI.
DR GO; GO:0006937; P:regulation of muscle contraction; ISO:MGI.
DR GO; GO:0006942; P:regulation of striated muscle contraction; ISO:MGI.
DR GO; GO:0045214; P:sarcomere organization; IBA:GO_Central.
DR GO; GO:0003009; P:skeletal muscle contraction; ISO:MGI.
DR Gene3D; 1.20.5.350; -; 1.
DR InterPro; IPR027707; TNNT.
DR InterPro; IPR027708; Tnnt3.
DR InterPro; IPR001978; Troponin.
DR InterPro; IPR038077; Troponin_sf.
DR PANTHER; PTHR11521; PTHR11521; 1.
DR PANTHER; PTHR11521:SF4; PTHR11521:SF4; 1.
DR Pfam; PF00992; Troponin; 1.
DR SUPFAM; SSF90250; SSF90250; 1.
PE 1: Evidence at protein level;
KW Acetylation; Alternative splicing; Muscle protein; Phosphoprotein;
KW Reference proteome.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250|UniProtKB:P02641"
FT CHAIN 2..272
FT /note="Troponin T, fast skeletal muscle"
FT /id="PRO_0000186179"
FT REGION 1..75
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 114..193
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 248..272
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..48
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 49..75
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 114..160
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 175..193
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 2
FT /note="N-acetylserine"
FT /evidence="ECO:0000250|UniProtKB:P02641"
FT MOD_RES 2
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P09739"
FT MOD_RES 91
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P09739"
FT MOD_RES 162
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 169
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P09739"
FT MOD_RES 170
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P09739"
FT MOD_RES 206
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P09739"
FT MOD_RES 222
FT /note="Phosphotyrosine"
FT /evidence="ECO:0000250|UniProtKB:P09739"
FT VAR_SEQ 11..16
FT /note="Missing (in isoform A4e17, isoform B3e17, isoform
FT B3e16, isoform B4e17 and isoform B4e16)"
FT /evidence="ECO:0000303|PubMed:9107680"
FT /id="VSP_050338"
FT VAR_SEQ 23..49
FT /note="Missing (in isoform B4e17 and isoform B4e16)"
FT /evidence="ECO:0000305"
FT /id="VSP_050340"
FT VAR_SEQ 23..31
FT /note="Missing (in isoform A3e17, isoform B2e17 and isoform
FT B2e16)"
FT /evidence="ECO:0000305"
FT /id="VSP_050339"
FT VAR_SEQ 28..31
FT /note="Missing (in isoform A2e17, isoform A4e17 and isoform
FT A6e17)"
FT /evidence="ECO:0000305"
FT /id="VSP_050341"
FT VAR_SEQ 32..49
FT /note="Missing (in isoform B1e16, isoform B3e17 and isoform
FT B3e16)"
FT /evidence="ECO:0000303|PubMed:9107680"
FT /id="VSP_050342"
FT VAR_SEQ 37..49
FT /note="Missing (in isoform A5e17, isoform A6e17, isoform
FT B2e17 and isoform B2e16)"
FT /evidence="ECO:0000305"
FT /id="VSP_050343"
FT VAR_SEQ 243..255
FT /note="TTLRSRIDQAQKH -> MTVRARVEMLAKF (in isoform B1e16,
FT isoform B2e16, isoform B3e16, isoform B4e16 and isoform
FT 14)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_050344"
FT VARIANT 131
FT /note="P -> R"
FT /evidence="ECO:0000269|PubMed:19468303"
FT CONFLICT 252
FT /note="A -> T (in Ref. 2; AAB39743)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 272 AA; 32241 MW; 5AF24573DD6A9529 CRC64;
MSDEETEQVE EQYEEEEEAQ EEEVQEEAPE PEEVQEDAVA EEEREEDEEE EKPRPKLTAP
KIPEGEKVDF DDIQKKRQNK DLMELQALID SHFEARKKEE EELIALKERI EKRRAERAEQ
QRIRAEKERE PQNRLAEEKA RREEEDAKRR AEDDMKKKKA LSSMGANYSS YLAKADQKRG
KKQTAREMKK KILAERRKPL NIDHLSDDKL RDKAKELWDT LYQLETDKFE FGEKLKRQKY
DITTLRSRID QAQKHSKKAG ATAKGKVGGR WK
