ID   TIM50_PHYIN             Reviewed;         409 AA.
AC   Q5S7T7;
DT   06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   21-DEC-2004, sequence version 1.
DT   25-MAY-2022, entry version 60.
DE   RecName: Full=Mitochondrial import inner membrane translocase subunit TIM50;
DE   AltName: Full=NIF domain protein 3;
DE   Flags: Precursor;
GN   Name=TIM50; Synonyms=NIFH3;
OS   Phytophthora infestans (Potato late blight agent) (Botrytis infestans).
OC   Eukaryota; Sar; Stramenopiles; Oomycota; Peronosporales; Peronosporaceae;
OC   Phytophthora.
OX   NCBI_TaxID=4787;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND DEVELOPMENTAL STAGE.
RX   PubMed=15588995; DOI=10.1016/j.fgb.2004.09.005;
RA   Tani S., Kim K.S., Judelson H.S.;
RT   "A cluster of NIF transcriptional regulators with divergent patterns of
RT   spore-specific expression in Phytophthora infestans.";
RL   Fungal Genet. Biol. 42:42-50(2005).
CC   -!- FUNCTION: Essential component of the TIM23 complex, a complex that
CC       mediates the translocation of transit peptide-containing proteins
CC       across the mitochondrial inner membrane. Required to direct preproteins
CC       in transit and direct them to the channel protein TIM23, and possibly
CC       facilitates transfer of the translocating proteins from the TOM complex
CC       to the TIM23 complex (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the TIM23 complex, at least composed of TIM23,
CC       TIM17 and TIM50. Interacts with preproteins in transit (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane {ECO:0000250};
CC       Single-pass membrane protein {ECO:0000250}.
CC   -!- DEVELOPMENTAL STAGE: Expressed during zoosporogenesis.
CC       {ECO:0000269|PubMed:15588995}.
CC   -!- SIMILARITY: Belongs to the TIM50 family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AY751575; AAV63943.1; -; mRNA.
DR   AlphaFoldDB; Q5S7T7; -.
DR   SMR; Q5S7T7; -.
DR   VEuPathDB; FungiDB:PITG_05927; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005744; C:TIM23 mitochondrial import inner membrane translocase complex; IEA:InterPro.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.1000; -; 1.
DR   InterPro; IPR004274; FCP1_dom.
DR   InterPro; IPR036412; HAD-like_sf.
DR   InterPro; IPR023214; HAD_sf.
DR   InterPro; IPR027111; Tim50.
DR   PANTHER; PTHR12210:SF3; PTHR12210:SF3; 1.
DR   Pfam; PF03031; NIF; 1.
DR   SMART; SM00577; CPDc; 1.
DR   SUPFAM; SSF56784; SSF56784; 1.
DR   PROSITE; PS50969; FCP1; 1.
PE   2: Evidence at transcript level;
KW   Membrane; Mitochondrion; Mitochondrion inner membrane; Protein transport;
KW   Transit peptide; Translocation; Transmembrane; Transmembrane helix;
KW   Transport.
FT   TRANSIT         1..63
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000255"
FT   CHAIN           64..409
FT                   /note="Mitochondrial import inner membrane translocase
FT                   subunit TIM50"
FT                   /id="PRO_0000043135"
FT   TOPO_DOM        64..89
FT                   /note="Mitochondrial matrix"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        90..109
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        110..409
FT                   /note="Mitochondrial intermembrane"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          164..307
FT                   /note="FCP1 homology"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00336"
SQ   SEQUENCE   409 AA;  45265 MW;  16D023FBEF4A9E90 CRC64;
     MMLKLVLRAQ RGMRAFAGIG ATRKLMLAAE ASAPSSVAEA SSIFAKVGRD IPLKVVPRAE
     RRAARKAAKH SADAATGHKV VRTSSLPARI SFALLAGSIS GSIVWNFVLD DGIKSRVKET
     LGATFLGDIY AIIAKKVEET VKPFTDPSRQ KLLPDWPIPQ VPADTPTVPV LVLDLEDTLV
     HSEWSRKHGW RHAKRPGVDE FLETLCQYYE IVIFSQNYGA EEIVQKLDPK QCALHILSRD
     ATRYLNGAHV KDLSNLNRDL RQVVILDDDP AAYQLQPENA IPVTPFTNGR DRDDHELKDL
     IPFLKALASE RVPDFRQVIG EFRDEDGVVR DLATKYGARV HQLEMQKEQK KKKGFGGFVR
     GRLSHHPASP TGGVAASGFS SGPHVLKRWT LLDSSAYVVA PRCIKEKCM