BRX_ARATH
ID BRX_ARATH Reviewed; 344 AA.
AC Q17TI5; Q17TI6; Q84JB6; Q9C6S9;
DT 05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT 05-MAY-2009, sequence version 2.
DT 03-AUG-2022, entry version 77.
DE RecName: Full=Protein BREVIS RADIX {ECO:0000303|PubMed:15031265};
DE Short=AtBRX {ECO:0000303|PubMed:15031265};
GN Name=BRX {ECO:0000303|PubMed:15031265};
GN OrderedLocusNames=At1g31880 {ECO:0000312|Araport:AT1G31880};
GN ORFNames=F5M6.11 {ECO:0000312|EMBL:AAG50720.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND SUBCELLULAR LOCATION.
RC STRAIN=cv. Uk-1, and cv. Uk-2;
RX PubMed=15031265; DOI=10.1101/gad.1187704;
RA Mouchel C.F., Briggs G.C., Hardtke C.S.;
RT "Natural genetic variation in Arabidopsis identifies BREVIS RADIX, a novel
RT regulator of cell proliferation and elongation in the root.";
RL Genes Dev. 18:700-714(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP TISSUE SPECIFICITY, AND INDUCTION BY AUXIN AND BRASSINOLIDE.
RX PubMed=17006513; DOI=10.1038/nature05130;
RA Mouchel C.F., Osmont K.S., Hardtke C.S.;
RT "BRX mediates feedback between brassinosteroid levels and auxin signalling
RT in root growth.";
RL Nature 443:458-461(2006).
RN [6]
RP GENE FAMILY, DOMAIN, AND SUBUNIT.
RX PubMed=16514016; DOI=10.1104/pp.105.075382;
RA Briggs G.C., Mouchel C.F., Hardtke C.S.;
RT "Characterization of the plant-specific BREVIS RADIX gene family reveals
RT limited genetic redundancy despite high sequence conservation.";
RL Plant Physiol. 140:1306-1316(2006).
RN [7]
RP FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, AND INTERACTION WITH
RP NGA1.
RX PubMed=19465596; DOI=10.1242/dev.035444;
RA Scacchi E., Osmont K.S., Beuchat J., Salinas P., Navarrete-Gomez M.,
RA Trigueros M., Ferrandiz C., Hardtke C.S.;
RT "Dynamic, auxin-responsive plasma membrane-to-nucleus movement of
RT Arabidopsis BRX.";
RL Development 136:2059-2067(2009).
RN [8]
RP DISRUPTION PHENOTYPE.
RX PubMed=19037657; DOI=10.1007/s00425-008-0854-6;
RA Li J., Mo X., Wang J., Chen N., Fan H., Dai C., Wu P.;
RT "BREVIS RADIX is involved in cytokinin-mediated inhibition of lateral root
RT initiation in Arabidopsis.";
RL Planta 229:593-603(2009).
RN [9]
RP FUNCTION, DISRUPTION PHENOTYPE, AND TISSUE SPECIFICITY.
RX PubMed=19201913; DOI=10.1104/pp.108.133819;
RA Rodrigues A., Santiago J., Rubio S., Saez A., Osmont K.S., Gadea J.,
RA Hardtke C.S., Rodriguez P.L.;
RT "The short-rooted phenotype of the brevis radix mutant partly reflects root
RT abscisic acid hypersensitivity.";
RL Plant Physiol. 149:1917-1928(2009).
RN [10]
RP FUNCTION, TISSUE SPECIFICITY, AND DISRUPTION PHENOTYPE.
RX PubMed=20649916; DOI=10.1111/j.1469-8137.2010.03387.x;
RA Beuchat J., Scacchi E., Tarkowska D., Ragni L., Strnad M., Hardtke C.S.;
RT "BRX promotes Arabidopsis shoot growth.";
RL New Phytol. 188:23-29(2010).
RN [11]
RP FUNCTION, TISSUE SPECIFICITY, AND INTERACTION WITH ARF5.
RX PubMed=21149702; DOI=10.1073/pnas.1014716108;
RA Scacchi E., Salinas P., Gujas B., Santuari L., Krogan N., Ragni L.,
RA Berleth T., Hardtke C.S.;
RT "Spatio-temporal sequence of cross-regulatory events in root meristem
RT growth.";
RL Proc. Natl. Acad. Sci. U.S.A. 107:22734-22739(2010).
RN [12]
RP FUNCTION, DISRUPTION PHENOTYPE, DEVELOPMENTAL STAGE, AND TISSUE
RP SPECIFICITY.
RC STRAIN=cv. Columbia;
RX PubMed=23569225; DOI=10.1073/pnas.1222314110;
RA Depuydt S., Rodriguez-Villalon A., Santuari L., Wyser-Rmili C., Ragni L.,
RA Hardtke C.S.;
RT "Suppression of Arabidopsis protophloem differentiation and root meristem
RT growth by CLE45 requires the receptor-like kinase BAM3.";
RL Proc. Natl. Acad. Sci. U.S.A. 110:7074-7079(2013).
RN [13]
RP FUNCTION, DISRUPTION PHENOTYPE, AND TISSUE SPECIFICITY.
RC STRAIN=cv. Columbia;
RX PubMed=25049386; DOI=10.1073/pnas.1407337111;
RA Rodriguez-Villalon A., Gujas B., Kang Y.H., Breda A.S., Cattaneo P.,
RA Depuydt S., Hardtke C.S.;
RT "Molecular genetic framework for protophloem formation.";
RL Proc. Natl. Acad. Sci. U.S.A. 111:11551-11556(2014).
RN [14]
RP DISRUPTION PHENOTYPE.
RC STRAIN=cv. Columbia;
RX PubMed=27354416; DOI=10.15252/embr.201642450;
RA Kang Y.H., Hardtke C.S.;
RT "Arabidopsis MAKR5 is a positive effector of BAM3-dependent CLE45
RT signaling.";
RL EMBO Rep. 17:1145-1154(2016).
RN [15]
RP FUNCTION, DISRUPTION PHENOTYPE, AND SUBCELLULAR LOCATION.
RC STRAIN=cv. Columbia;
RX PubMed=28652362; DOI=10.1073/pnas.1703258114;
RA Breda A.S., Hazak O., Hardtke C.S.;
RT "Phosphosite charge rather than shootward localization determines OCTOPUS
RT activity in root protophloem.";
RL Proc. Natl. Acad. Sci. U.S.A. 114:E5721-E5730(2017).
CC -!- FUNCTION: Acts as a regulator of cell proliferation and elongation in
CC the root and shoot (PubMed:25049386, PubMed:28652362). Regulates roots
CC architecture and primary root protophloem differentiation
CC (PubMed:25049386, PubMed:28652362). BRX, BAM3, and CLE45 act together
CC to regulate the transition of protophloem cells from proliferation to
CC differentiation, thus impinging on postembryonic growth capacity of the
CC root meristem (PubMed:23569225). Probable transcription regulator.
CC Regulated by the auxin response factor ARF5. Polarly localized in
CC vascular cells and subject to endocytic recycling. Required for CPD
CC expression and for correct nuclear auxin response. Mediates cross-talk
CC between the auxin and brassinosteroid pathways. BRX is a target for
CC auxin-induced, proteasome-mediated degradation.
CC {ECO:0000269|PubMed:15031265, ECO:0000269|PubMed:19201913,
CC ECO:0000269|PubMed:19465596, ECO:0000269|PubMed:20649916,
CC ECO:0000269|PubMed:21149702, ECO:0000269|PubMed:23569225,
CC ECO:0000269|PubMed:25049386, ECO:0000269|PubMed:28652362}.
CC -!- SUBUNIT: Homodimer and heterodimer with BRXL1. Interacts with NGA1 and
CC ARF5. {ECO:0000269|PubMed:16514016, ECO:0000269|PubMed:19465596,
CC ECO:0000269|PubMed:21149702}.
CC -!- INTERACTION:
CC Q17TI5; Q9S7Z2: AFP4; NbExp=3; IntAct=EBI-4426649, EBI-1778843;
CC Q17TI5; Q9FLI3: AHG1; NbExp=3; IntAct=EBI-4426649, EBI-2363348;
CC Q17TI5; Q8LPN5: AHL12; NbExp=3; IntAct=EBI-4426649, EBI-4428689;
CC Q17TI5; Q9LKL2: APRR1; NbExp=3; IntAct=EBI-4426649, EBI-618423;
CC Q17TI5; Q8L9Y3: ARR14; NbExp=3; IntAct=EBI-4426649, EBI-1100737;
CC Q17TI5; Q949T8: ASHR3; NbExp=3; IntAct=EBI-4426649, EBI-4457339;
CC Q17TI5; Q681Q7: At1g03900; NbExp=4; IntAct=EBI-4426649, EBI-4440643;
CC Q17TI5; Q9SHJ1: At1g06460; NbExp=3; IntAct=EBI-4426649, EBI-25516773;
CC Q17TI5; Q9FWW2: At1g12120; NbExp=3; IntAct=EBI-4426649, EBI-25518624;
CC Q17TI5; A0A178WMH3: At1g23220; NbExp=3; IntAct=EBI-4426649, EBI-25519119;
CC Q17TI5; Q9SLL2: At1g54200; NbExp=3; IntAct=EBI-4426649, EBI-25516987;
CC Q17TI5; Q9SGS5: At1g76070; NbExp=3; IntAct=EBI-4426649, EBI-25519221;
CC Q17TI5; O64764: At2g35010; NbExp=3; IntAct=EBI-4426649, EBI-25519089;
CC Q17TI5; Q9LUS8: At3g16560; NbExp=3; IntAct=EBI-4426649, EBI-25519200;
CC Q17TI5; Q9LS61: At3g18295; NbExp=3; IntAct=EBI-4426649, EBI-21457081;
CC Q17TI5; F4JI72: At4g03250; NbExp=3; IntAct=EBI-4426649, EBI-15192535;
CC Q17TI5; A0A1P8B4Y6: At4g21660; NbExp=3; IntAct=EBI-4426649, EBI-25519178;
CC Q17TI5; Q9XFH9: At5g16400; NbExp=3; IntAct=EBI-4426649, EBI-25516100;
CC Q17TI5; Q6NM33: At5g19340; NbExp=3; IntAct=EBI-4426649, EBI-25518939;
CC Q17TI5; A0A178UKR9: At5g50180; NbExp=3; IntAct=EBI-4426649, EBI-25518767;
CC Q17TI5; Q9LTI1: At5g59490; NbExp=3; IntAct=EBI-4426649, EBI-25518827;
CC Q17TI5; A0A384LJI7: AXX17_At1g00240; NbExp=3; IntAct=EBI-4426649, EBI-25518871;
CC Q17TI5; A0A178W725: AXX17_At1g45300; NbExp=3; IntAct=EBI-4426649, EBI-25515760;
CC Q17TI5; A0A384KYS8: AXX17_At3g42630; NbExp=3; IntAct=EBI-4426649, EBI-25517043;
CC Q17TI5; Q7XJU2: BHLH153; NbExp=5; IntAct=EBI-4426649, EBI-4427748;
CC Q17TI5; Q9LEZ3: BIM1; NbExp=5; IntAct=EBI-4426649, EBI-617095;
CC Q17TI5; Q9LDE2: BPC2; NbExp=3; IntAct=EBI-4426649, EBI-15215888;
CC Q17TI5; Q9SGS4: CDSP32; NbExp=3; IntAct=EBI-4426649, EBI-25517863;
CC Q17TI5; Q9SEZ7: CIPK16; NbExp=3; IntAct=EBI-4426649, EBI-1573415;
CC Q17TI5; Q9MAM1: CIPK9; NbExp=4; IntAct=EBI-4426649, EBI-1765282;
CC Q17TI5; Q9LFR7: CXE17; NbExp=5; IntAct=EBI-4426649, EBI-4456165;
CC Q17TI5; Q9FG74: D6PK; NbExp=3; IntAct=EBI-4426649, EBI-1103570;
CC Q17TI5; Q9SUA3: D6PKL1; NbExp=3; IntAct=EBI-4426649, EBI-1103605;
CC Q17TI5; Q05999: D6PKL3; NbExp=4; IntAct=EBI-4426649, EBI-1103648;
CC Q17TI5; O23263: dl3050c; NbExp=3; IntAct=EBI-4426649, EBI-25519155;
CC Q17TI5; Q8GY79: DRB5; NbExp=3; IntAct=EBI-4426649, EBI-632672;
CC Q17TI5; Q84MD6: DSP2; NbExp=6; IntAct=EBI-4426649, EBI-4424266;
CC Q17TI5; Q56XH8: ECT11; NbExp=3; IntAct=EBI-4426649, EBI-25516971;
CC Q17TI5; Q9FHK4: EDR4; NbExp=3; IntAct=EBI-4426649, EBI-1544548;
CC Q17TI5; F4J6A1: EIF3G1; NbExp=3; IntAct=EBI-4426649, EBI-25518734;
CC Q17TI5; Q9MAI5: ERF8; NbExp=3; IntAct=EBI-4426649, EBI-2000137;
CC Q17TI5; Q9ZSZ8: FIP37; NbExp=4; IntAct=EBI-4426649, EBI-1641243;
CC Q17TI5; Q9LYE4: FLZ10; NbExp=3; IntAct=EBI-4426649, EBI-25519036;
CC Q17TI5; Q39103: GA3OX1; NbExp=5; IntAct=EBI-4426649, EBI-4426378;
CC Q17TI5; F4KGE8: GIL1; NbExp=3; IntAct=EBI-4426649, EBI-25519238;
CC Q17TI5; Q8LBK6: GRXS15; NbExp=3; IntAct=EBI-4426649, EBI-4427398;
CC Q17TI5; Q9SAC6: GWD1; NbExp=3; IntAct=EBI-4426649, EBI-2355356;
CC Q17TI5; Q9LZR5: HDT3; NbExp=3; IntAct=EBI-4426649, EBI-3387398;
CC Q17TI5; Q9M4T3: HDT4; NbExp=3; IntAct=EBI-4426649, EBI-25518903;
CC Q17TI5; Q9C9X7: IDD14; NbExp=3; IntAct=EBI-4426649, EBI-15191579;
CC Q17TI5; Q948J9: LIP2P; NbExp=3; IntAct=EBI-4426649, EBI-25519006;
CC Q17TI5; Q1H595: LSM2; NbExp=3; IntAct=EBI-4426649, EBI-9347308;
CC Q17TI5; Q5XEN5: MBD1; NbExp=3; IntAct=EBI-4426649, EBI-4445335;
CC Q17TI5; O80397: MKK4; NbExp=6; IntAct=EBI-4426649, EBI-2358409;
CC Q17TI5; Q9C5C0: MPK18; NbExp=3; IntAct=EBI-4426649, EBI-1238534;
CC Q17TI5; Q9SJG9: MPK20; NbExp=5; IntAct=EBI-4426649, EBI-2358896;
CC Q17TI5; Q93ZM2: MWD9.9; NbExp=4; IntAct=EBI-4426649, EBI-4428808;
CC Q17TI5; Q9SEZ4: MYB105; NbExp=3; IntAct=EBI-4426649, EBI-15202260;
CC Q17TI5; Q9S9K9: MYB3; NbExp=3; IntAct=EBI-4426649, EBI-1786591;
CC Q17TI5; Q8VYP8: MYJ24.12; NbExp=3; IntAct=EBI-4426649, EBI-4442894;
CC Q17TI5; Q9FK59: NCBP; NbExp=3; IntAct=EBI-4426649, EBI-1770573;
CC Q17TI5; O82799: NGA1; NbExp=6; IntAct=EBI-4426649, EBI-4427936;
CC Q17TI5; P11035: NIA2; NbExp=3; IntAct=EBI-4426649, EBI-4451150;
CC Q17TI5; Q9SWI1: PKS1; NbExp=3; IntAct=EBI-4426649, EBI-626200;
CC Q17TI5; Q9M9T4: PKS2; NbExp=3; IntAct=EBI-4426649, EBI-25512733;
CC Q17TI5; Q9FYE2: PKS4; NbExp=3; IntAct=EBI-4426649, EBI-25513821;
CC Q17TI5; Q9S7H1: psaD1; NbExp=3; IntAct=EBI-4426649, EBI-1805558;
CC Q17TI5; Q9C8D1: PUB20; NbExp=3; IntAct=EBI-4426649, EBI-25516153;
CC Q17TI5; Q058P4: PUB30; NbExp=3; IntAct=EBI-4426649, EBI-4442587;
CC Q17TI5; Q852U6: RHY1A; NbExp=4; IntAct=EBI-4426649, EBI-4428791;
CC Q17TI5; Q8GY88: SK2; NbExp=3; IntAct=EBI-4426649, EBI-1238334;
CC Q17TI5; Q0WT24: STOP2; NbExp=3; IntAct=EBI-4426649, EBI-4424123;
CC Q17TI5; Q9LVG2: TOE2; NbExp=4; IntAct=EBI-4426649, EBI-4424568;
CC Q17TI5; Q9SZG3: VQ29; NbExp=3; IntAct=EBI-4426649, EBI-25517843;
CC Q17TI5; Q0WQX9: WAVH2; NbExp=3; IntAct=EBI-4426649, EBI-4426718;
CC Q17TI5; Q93WY4: WRKY12; NbExp=3; IntAct=EBI-4426649, EBI-25513081;
CC Q17TI5; Q9SJA8: WRKY17; NbExp=6; IntAct=EBI-4426649, EBI-2365037;
CC Q17TI5; Q9CAR4: WRKY36; NbExp=3; IntAct=EBI-4426649, EBI-15210240;
CC Q17TI5; Q9FQ04: XRN4; NbExp=3; IntAct=EBI-4426649, EBI-25511549;
CC -!- SUBCELLULAR LOCATION: Nucleus. Cell membrane
CC {ECO:0000269|PubMed:28652362}; Peripheral membrane protein; Cytoplasmic
CC side. Note=The translocation to the nucleus depends on the auxin
CC concentration. Polar localization at the plasma membrane, rootward
CC oriented, in developing root protophloem cells (PubMed:28652362).
CC {ECO:0000269|PubMed:28652362}.
CC -!- TISSUE SPECIFICITY: Expressed in the developing protophloem up to the
CC elongation zone in root meristem of young seedlings, in the columella
CC and the phloem vasculature throughout the root and in the phloem
CC vasculature in the shoot (PubMed:23569225). Detected in the shoot
CC meristem and in primordia. Low expression in stomata. Confined to sieve
CC element precursor cells and to protophloem (PubMed:25049386).
CC {ECO:0000269|PubMed:17006513, ECO:0000269|PubMed:19201913,
CC ECO:0000269|PubMed:20649916, ECO:0000269|PubMed:21149702,
CC ECO:0000269|PubMed:23569225, ECO:0000269|PubMed:25049386}.
CC -!- DEVELOPMENTAL STAGE: Ubiquitously expressed in early embryos and
CC becomes restricted to the vasculature in the mature embryo and at later
CC stages of development (PubMed:19465596). Expressed in roots developing
CC protophloem, up to the end of the transition zone, as well as in the
CC root tip meristem (PubMed:23569225). {ECO:0000269|PubMed:19465596,
CC ECO:0000269|PubMed:23569225}.
CC -!- INDUCTION: Up-regulated by auxin and down-regulated by brassinolide.
CC {ECO:0000269|PubMed:17006513}.
CC -!- DOMAIN: The DZC domain is necessary and sufficient for dimerization. A
CC C-terminal fragment containing both DZC domains is able to rescue the
CC short root phenotype. The N-terminal part (1-57) promotes BRX membrane
CC association and is required for efficient degradation in the nucleus.
CC {ECO:0000269|PubMed:16514016}.
CC -!- DISRUPTION PHENOTYPE: Impaired primary root protophloem
CC differentiation. Short primary root and increased number of lateral
CC roots (PubMed:25049386, PubMed:28652362). Absent sieve element
CC precursor division, occurrence of gap cells lacking actin filament,
CC associated with a reduction in the number of early dividing protophloem
CC cells, in root meristem size and root growth; these phenotypes are
CC partially rescued in plants also missing MAKR5 (PubMed:25049386,
CC PubMed:28652362, PubMed:27354416). Patchy expression of SUC2
CC (PubMed:25049386). Reduced cotyledon and leaf size. Enhanced response
CC to abscisic acid-mediated inhibition of root growth and insensitivity
CC to cytokinin induced inhibition of lateral root initiation. The double
CC mutant brx ops double mutant has strongly reduced root length and
CC meristem size, with missing protophloem strands (PubMed:28652362). The
CC disruption of BAM3 restores root protophloem and mersitem phenotypes
CC observed in brx mutants (PubMed:23569225). Ectopic overexpression of
CC BAM3 but slightly reduced CLE45 levels in root meristems
CC (PubMed:23569225). {ECO:0000269|PubMed:19037657,
CC ECO:0000269|PubMed:19201913, ECO:0000269|PubMed:20649916,
CC ECO:0000269|PubMed:23569225, ECO:0000269|PubMed:25049386,
CC ECO:0000269|PubMed:27354416, ECO:0000269|PubMed:28652362}.
CC -!- MISCELLANEOUS: The truncated variant in cv. Uk-1 results in short root
CC phenotype.
CC -!- SIMILARITY: Belongs to the BRX family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAG50720.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AY702648; ABG25052.1; -; mRNA.
DR EMBL; AY702649; ABG25053.1; -; mRNA.
DR EMBL; AC079041; AAG50720.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE31412.1; -; Genomic_DNA.
DR EMBL; BT002854; AAO22671.1; -; mRNA.
DR EMBL; BT004390; AAO42384.1; -; mRNA.
DR PIR; A86443; A86443.
DR RefSeq; NP_174471.2; NM_102925.3.
DR AlphaFoldDB; Q17TI5; -.
DR SMR; Q17TI5; -.
DR BioGRID; 25312; 96.
DR IntAct; Q17TI5; 94.
DR STRING; 3702.AT1G31880.1; -.
DR PaxDb; Q17TI5; -.
DR PRIDE; Q17TI5; -.
DR ProteomicsDB; 240364; -.
DR EnsemblPlants; AT1G31880.1; AT1G31880.1; AT1G31880.
DR GeneID; 840078; -.
DR Gramene; AT1G31880.1; AT1G31880.1; AT1G31880.
DR KEGG; ath:AT1G31880; -.
DR Araport; AT1G31880; -.
DR TAIR; locus:2034486; AT1G31880.
DR eggNOG; ENOG502QU4N; Eukaryota.
DR HOGENOM; CLU_033380_0_1_1; -.
DR InParanoid; Q17TI5; -.
DR PhylomeDB; Q17TI5; -.
DR PRO; PR:Q17TI5; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q17TI5; baseline and differential.
DR Genevisible; Q17TI5; AT.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0042802; F:identical protein binding; IPI:TAIR.
DR GO; GO:0010315; P:auxin export across the plasma membrane; IDA:TAIR.
DR GO; GO:0009734; P:auxin-activated signaling pathway; IMP:TAIR.
DR GO; GO:0030154; P:cell differentiation; IMP:UniProtKB.
DR GO; GO:0009736; P:cytokinin-activated signaling pathway; IMP:TAIR.
DR GO; GO:0048527; P:lateral root development; IMP:TAIR.
DR GO; GO:0010078; P:maintenance of root meristem identity; IMP:UniProtKB.
DR GO; GO:0010088; P:phloem development; IMP:UniProtKB.
DR GO; GO:2000280; P:regulation of root development; IMP:UniProtKB.
DR GO; GO:0009737; P:response to abscisic acid; IMP:TAIR.
DR GO; GO:0048364; P:root development; IMP:TAIR.
DR GO; GO:0048756; P:sieve cell differentiation; IMP:TAIR.
DR InterPro; IPR013591; Brevis_radix_dom.
DR InterPro; IPR044532; BRX-like.
DR InterPro; IPR027988; BRX_N.
DR PANTHER; PTHR46058; PTHR46058; 1.
DR Pfam; PF08381; BRX; 2.
DR Pfam; PF13713; BRX_N; 1.
DR PROSITE; PS51514; BRX; 2.
PE 1: Evidence at protein level;
KW Cell membrane; Developmental protein; Differentiation; Membrane; Nucleus;
KW Reference proteome; Repeat.
FT CHAIN 1..344
FT /note="Protein BREVIS RADIX"
FT /id="PRO_0000373821"
FT DOMAIN 139..194
FT /note="BRX 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00847"
FT DOMAIN 289..344
FT /note="BRX 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00847"
FT REGION 203..286
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 214..234
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 267..286
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VARIANT 141..344
FT /note="Missing (in strain: cv. Uk-1)"
FT VARIANT 310
FT /note="T -> S (in strain: cv. Uk-2)"
SQ SEQUENCE 344 AA; 38761 MW; D36075D26158FFD7 CRC64;
MFSCIACTKA DGGEEVEHGA RGGTTPNTKE AVKSLTIQIK DMALKFSGAY KQCKPCTGSS
SSPLKKGHRS FPDYDNASEG VPYPFMGGSA GSTPAWDFTN SSHHPAGRLE SKFTSIYGND
RESISAQSCD VVLDDDGPKE WMAQVEPGVH ITFASLPTGG NDLKRIRFSR EMFDKWQAQR
WWGENYDKIV ELYNVQRFNR QALQTPARSD DQSQRDSTYS KMDSARESKD WTPRHNFRPP
GSVPHHFYGG SSNYGPGSYH GGPPMDAART TTSSRDDPPS MSNASEMQAE WIEEDEPGVY
ITIRQLSDGT RELRRVRFSR ERFGEVHAKT WWEQNRERIQ TQYL
