THUM2_MOUSE
ID THUM2_MOUSE Reviewed; 528 AA.
AC Q9CZB3;
DT 05-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT 27-JUL-2011, sequence version 3.
DT 03-AUG-2022, entry version 120.
DE RecName: Full=THUMP domain-containing protein 2;
GN Name=Thumpd2;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Embryo;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
CC -!- SUBUNIT: Interacts with TRMT112. {ECO:0000250|UniProtKB:Q9BTF0}.
CC -!- SIMILARITY: Belongs to the methyltransferase superfamily.
CC {ECO:0000305}.
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DR EMBL; AK012806; BAB28484.1; -; mRNA.
DR EMBL; AC165966; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR CCDS; CCDS50190.1; -.
DR RefSeq; NP_082414.1; NM_028138.1.
DR AlphaFoldDB; Q9CZB3; -.
DR SMR; Q9CZB3; -.
DR IntAct; Q9CZB3; 1.
DR STRING; 10090.ENSMUSP00000025093; -.
DR iPTMnet; Q9CZB3; -.
DR PhosphoSitePlus; Q9CZB3; -.
DR EPD; Q9CZB3; -.
DR jPOST; Q9CZB3; -.
DR MaxQB; Q9CZB3; -.
DR PaxDb; Q9CZB3; -.
DR PeptideAtlas; Q9CZB3; -.
DR PRIDE; Q9CZB3; -.
DR ProteomicsDB; 258877; -.
DR Antibodypedia; 29662; 52 antibodies from 17 providers.
DR Ensembl; ENSMUST00000025093; ENSMUSP00000025093; ENSMUSG00000024246.
DR GeneID; 72167; -.
DR KEGG; mmu:72167; -.
DR UCSC; uc008drp.2; mouse.
DR CTD; 80745; -.
DR MGI; MGI:1919417; Thumpd2.
DR VEuPathDB; HostDB:ENSMUSG00000024246; -.
DR eggNOG; ENOG502QSR4; Eukaryota.
DR GeneTree; ENSGT00530000063557; -.
DR HOGENOM; CLU_045692_1_0_1; -.
DR InParanoid; Q9CZB3; -.
DR OMA; QWTSAVM; -.
DR OrthoDB; 674030at2759; -.
DR PhylomeDB; Q9CZB3; -.
DR TreeFam; TF313093; -.
DR BioGRID-ORCS; 72167; 2 hits in 74 CRISPR screens.
DR ChiTaRS; Thumpd2; mouse.
DR PRO; PR:Q9CZB3; -.
DR Proteomes; UP000000589; Chromosome 17.
DR RNAct; Q9CZB3; protein.
DR Bgee; ENSMUSG00000024246; Expressed in ventricular zone and 131 other tissues.
DR ExpressionAtlas; Q9CZB3; baseline and differential.
DR Genevisible; Q9CZB3; MM.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0016423; F:tRNA (guanine) methyltransferase activity; IBA:GO_Central.
DR GO; GO:0030488; P:tRNA methylation; IBA:GO_Central.
DR Gene3D; 3.40.50.150; -; 1.
DR InterPro; IPR000241; RNA_methylase_dom.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR004114; THUMP_dom.
DR Pfam; PF02926; THUMP; 1.
DR Pfam; PF01170; UPF0020; 1.
DR SMART; SM00981; THUMP; 1.
DR SUPFAM; SSF53335; SSF53335; 1.
DR PROSITE; PS51165; THUMP; 1.
PE 2: Evidence at transcript level;
KW Methyltransferase; Reference proteome; RNA-binding; Transferase.
FT CHAIN 1..528
FT /note="THUMP domain-containing protein 2"
FT /id="PRO_0000072533"
FT DOMAIN 162..266
FT /note="THUMP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00529"
FT REGION 154..200
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 437..460
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 180..200
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 444..460
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 528 AA; 57669 MW; 6242BC0BC3976CC5 CRC64;
MAVAQADPGS DPAGGARFFC TAGRGLEPFL MREVRARLEA TQVEYISGKV FFTTCSDLPS
MKKLKSAERL FVLIKKQLPI AVSSLHKGKI LNEMQRFVND DPGSWLEAIS LWKKLLEHDP
KREKVSQRDA NPLKRKAGEN ETIIAKKLKV EEKQEVAKDH GESQEDKLLQ GSPEQGEAVT
RTELQDHRLN STTEKAADAQ DQEDLTFRVS CRCTGNVRKV ITGQEAGRVI GLALMRQFGW
KADLRNPNIE IFMHLSDAYS VVGIPLLRMP LASRTYIQTA GLRSTIAWAM ASIAEIKAGA
LVLDPMCGLG TILVEAAEEW PDVFYMGADM SDAQLLGACD NLKAAGLTDR IELLKSSVTD
LPLPSQSTDV IISDIPFGKK FKLGKDIKSI LQEMERVLRV GGAMVLLLSE DHHRHLTDCG
GSSIPLTSKG HIAEPEMKTL SNPDRTGAPD TASPSQRASS VQCLGRMRPC GSLVPVESFK
VSLGKTDAFI CKYKKAQASG LSSAECHEPG AHTEMAAMQE SPSLQDSL
