ID   THN1_WHEAT              Reviewed;         126 AA.
AC   P01544;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1993, sequence version 2.
DT   03-AUG-2022, entry version 116.
DE   RecName: Full=Alpha-1-purothionin;
DE   AltName: Full=Purothionin A-II;
DE   Contains:
DE     RecName: Full=Alpha-1-purothionin;
DE   Contains:
DE     RecName: Full=Acidic protein;
DE   Flags: Precursor; Fragment;
GN   Name=THI1.1; Synonyms=PUR-D1;
OS   Triticum aestivum (Wheat).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Pooideae; Triticodae; Triticeae; Triticinae; Triticum.
OX   NCBI_TaxID=4565;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Endosperm;
RX   PubMed=7824649; DOI=10.1104/pp.106.3.1221;
RA   Castagnaro A., Marana C., Carbonero P., Garcia-Olmedo F.;
RT   "cDNA cloning and nucleotide sequences of alpha 1 and alpha 2 thionins from
RT   hexaploid wheat endosperm.";
RL   Plant Physiol. 106:1221-1222(1994).
RN   [2]
RP   PROTEIN SEQUENCE OF 17-61.
RC   STRAIN=cv. Manitoba 3;
RX   PubMed=914810; DOI=10.1093/oxfordjournals.jbchem.a131752;
RA   Ohtani S., Okada T., Yoshizumi H., Kagamiyama H.;
RT   "Complete primary structures of two subunits of purothionin A, a lethal
RT   protein for brewer's yeast from wheat flour.";
RL   J. Biochem. 82:753-767(1977).
RN   [3]
RP   PROTEIN SEQUENCE OF 17-61.
RA   Ohtani S., Okada T., Kagamiyama H., Yoshizumi H.;
RT   "The amino acid sequence of purothionin A, a lethal toxic protein to
RT   brewer's yeast from wheat.";
RL   Agric. Biol. Chem. 39:2269-2270(1975).
RN   [4]
RP   PROTEIN SEQUENCE OF 17-61.
RC   STRAIN=cv. Manitou;
RA   Jones B.L., Mak A.S.;
RT   "Amino acid sequences of the two alpha-purothionins of hexaploid wheat.";
RL   Cereal Chem. 54:511-523(1977).
RN   [5]
RP   X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).
RX   PubMed=2235992; DOI=10.1002/prot.340080203;
RA   Teeter M.M., Ma X.-Q., Rao U., Whitlow M.;
RT   "Crystal structure of a protein-toxin alpha 1-purothionin at 2.5A and a
RT   comparison with predicted models.";
RL   Proteins 8:118-132(1990).
CC   -!- FUNCTION: Thionins are small plant proteins which are toxic to animal
CC       cells. They seem to exert their toxic effect at the level of the cell
CC       membrane. Their precise function is not known.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- SIMILARITY: Belongs to the plant thionin (TC 1.C.44) family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; X70666; CAA50004.1; -; mRNA.
DR   PDB; 2PLH; X-ray; 2.50 A; A=17-61.
DR   PDBsum; 2PLH; -.
DR   AlphaFoldDB; P01544; -.
DR   BMRB; P01544; -.
DR   SMR; P01544; -.
DR   STRING; 4565.Traes_1BL_5E6FB0425.1; -.
DR   Allergome; 9834; Tri a 37.
DR   PRIDE; P01544; -.
DR   EvolutionaryTrace; P01544; -.
DR   Proteomes; UP000019116; Unplaced.
DR   ExpressionAtlas; P01544; baseline.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.1350.10; -; 1.
DR   InterPro; IPR001010; Thionin.
DR   InterPro; IPR036391; Thionin-like_sf.
DR   PANTHER; PTHR33920; PTHR33920; 1.
DR   Pfam; PF00321; Thionin; 1.
DR   PRINTS; PR00287; THIONIN.
DR   SUPFAM; SSF57429; SSF57429; 1.
DR   PROSITE; PS00271; THIONIN; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Direct protein sequencing; Disulfide bond; Plant defense;
KW   Reference proteome; Secreted; Signal; Toxin.
FT   SIGNAL          <1..16
FT                   /evidence="ECO:0000269|PubMed:914810, ECO:0000269|Ref.3,
FT                   ECO:0000269|Ref.4"
FT   CHAIN           17..61
FT                   /note="Alpha-1-purothionin"
FT                   /id="PRO_0000034126"
FT   CHAIN           62..126
FT                   /note="Acidic protein"
FT                   /id="PRO_0000034127"
FT   DISULFID        19..55
FT   DISULFID        20..47
FT   DISULFID        28..45
FT   DISULFID        32..41
FT   NON_TER         1
FT   STRAND          18..22
FT                   /evidence="ECO:0007829|PDB:2PLH"
FT   HELIX           23..32
FT                   /evidence="ECO:0007829|PDB:2PLH"
FT   TURN            33..35
FT                   /evidence="ECO:0007829|PDB:2PLH"
FT   HELIX           38..45
FT                   /evidence="ECO:0007829|PDB:2PLH"
FT   STRAND          47..49
FT                   /evidence="ECO:0007829|PDB:2PLH"
FT   STRAND          51..54
FT                   /evidence="ECO:0007829|PDB:2PLH"
SQ   SEQUENCE   126 AA;  13526 MW;  FF7310D921C4EE30 CRC64;
     CLLILGLVLE QLQVEGKSCC RSTLGRNCYN LCRARGAQKL CAGVCRCKIS SGLSCPKGFP
     KLALESNSDE PDTIEYCNLG CRSSVCDYMV NAAADDEEMK LYVENCADAC VSFCNGDAGL
     PSLDAY