ID   BREU_EUPCP              Reviewed;          24 AA.
AC   C0HKQ2;
DT   05-JUL-2017, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2017, sequence version 1.
DT   25-MAY-2022, entry version 4.
DE   RecName: Full=Brevinin-Eu {ECO:0000303|PubMed:24168384};
OS   Euphlyctis cyanophlyctis (Skittering frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Neobatrachia; Ranoidea; Dicroglossidae; Dicroglossinae;
OC   Euphlyctis.
OX   NCBI_TaxID=58519 {ECO:0000303|PubMed:24168384};
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, FUNCTION, SUBCELLULAR LOCATION, MASS SPECTROMETRY, AND
RP   IDENTIFICATION BY MASS SPECTROMETRY.
RC   TISSUE=Skin secretion {ECO:0000303|PubMed:24168384};
RX   PubMed=24168384; DOI=10.1111/cbdd.12256;
RA   Asoodeh A., Sepahi S., Ghorani-Azam A.;
RT   "Purification and modeling amphipathic alpha helical antimicrobial peptides
RT   from skin secretions of Euphlyctis cyanophlyctis.";
RL   Chem. Biol. Drug Des. 83:411-417(2014).
CC   -!- FUNCTION: Antimicrobial peptide active against E.coli (MIC=8 uM),
CC       K.pneumoniae (MIC=14 uM), B.cereus (MIC=12 uM) and S.aureus (MIC=17
CC       uM). Has very little hemolytic activity. {ECO:0000269|PubMed:24168384}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:24168384}.
CC   -!- TISSUE SPECIFICITY: Expressed by the skin glands.
CC       {ECO:0000305|PubMed:24168384}.
CC   -!- MASS SPECTROMETRY: Mass=2610.1; Mass_error=1; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:24168384};
CC   -!- SIMILARITY: Belongs to the frog skin active peptide (FSAP) family.
CC       Brevinin subfamily. {ECO:0000305}.
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DR   AlphaFoldDB; C0HKQ2; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR   InterPro; IPR012520; Antimicrobial_frog_1.
DR   Pfam; PF08018; Antimicrobial_1; 1.
PE   1: Evidence at protein level;
KW   Amphibian defense peptide; Antibiotic; Antimicrobial;
KW   Direct protein sequencing; Disulfide bond; Secreted.
FT   PEPTIDE         1..24
FT                   /note="Brevinin-Eu"
FT                   /evidence="ECO:0000269|PubMed:24168384"
FT                   /id="PRO_0000441012"
FT   DISULFID        18..24
FT                   /evidence="ECO:0000250|UniProtKB:P86028"
SQ   SEQUENCE   24 AA;  2610 MW;  DE5A59E769FDAD5F CRC64;
     VIPFVASVAA EMMQHIFCAA SRKC