ID   TGDS_DICDI              Reviewed;         434 AA.
AC   Q54WS6;
DT   08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   03-AUG-2022, entry version 95.
DE   RecName: Full=dTDP-D-glucose 4,6-dehydratase;
DE            EC=4.2.1.46;
GN   Name=tgds; ORFNames=DDB_G0279465;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=dTDP-alpha-D-glucose = dTDP-4-dehydro-6-deoxy-alpha-D-glucose
CC         + H2O; Xref=Rhea:RHEA:17221, ChEBI:CHEBI:15377, ChEBI:CHEBI:57477,
CC         ChEBI:CHEBI:57649; EC=4.2.1.46;
CC   -!- COFACTOR:
CC       Name=NAD(+); Xref=ChEBI:CHEBI:57540; Evidence={ECO:0000250};
CC   -!- SIMILARITY: Belongs to the NAD(P)-dependent epimerase/dehydratase
CC       family. dTDP-glucose dehydratase subfamily. {ECO:0000305}.
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DR   EMBL; AAFI02000031; EAL67671.1; -; Genomic_DNA.
DR   RefSeq; XP_641641.1; XM_636549.1.
DR   AlphaFoldDB; Q54WS6; -.
DR   SMR; Q54WS6; -.
DR   STRING; 44689.DDB0231748; -.
DR   PaxDb; Q54WS6; -.
DR   EnsemblProtists; EAL67671; EAL67671; DDB_G0279465.
DR   GeneID; 8622047; -.
DR   KEGG; ddi:DDB_G0279465; -.
DR   dictyBase; DDB_G0279465; tgds.
DR   eggNOG; KOG0747; Eukaryota.
DR   HOGENOM; CLU_007383_1_14_1; -.
DR   InParanoid; Q54WS6; -.
DR   OMA; KLIPLMC; -.
DR   PhylomeDB; Q54WS6; -.
DR   PRO; PR:Q54WS6; -.
DR   Proteomes; UP000002195; Chromosome 3.
DR   GO; GO:0008460; F:dTDP-glucose 4,6-dehydratase activity; IBA:GO_Central.
DR   GO; GO:0009225; P:nucleotide-sugar metabolic process; IEA:InterPro.
DR   CDD; cd05246; dTDP_GD_SDR_e; 1.
DR   InterPro; IPR005888; dTDP_Gluc_deHydtase.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   Pfam; PF16363; GDP_Man_Dehyd; 1.
DR   SUPFAM; SSF51735; SSF51735; 1.
PE   3: Inferred from homology;
KW   Lyase; NAD; Reference proteome.
FT   CHAIN           1..434
FT                   /note="dTDP-D-glucose 4,6-dehydratase"
FT                   /id="PRO_0000328214"
FT   REGION          286..310
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        135
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        136
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        169
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250"
FT   BINDING         134
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   434 AA;  50332 MW;  81F4935E8CD8781A CRC64;
     MNNKDDLFIQ KILITGGAGF IGSHLAIYLT KKFKNSKIIV LDKLDYCSNI NNLGCVLKEL
     NFKFYKGNIL DSELLENIFE KEKIDIVIHL AAYTHVDNSF KQSIKFTENN ILGTHYLLET
     CKNYKLKKFI YVSTDEVYGS GLIEDNDDNN NSINQSSNEK SILNPTNPYS ASKAGAEHLV
     QSYYKSFKLP VIITRANNIY GPKQYPEKII PKFINLLLNN KKCTIHGTGK NTRNYLYIDD
     IVSAFDIILR KGEIGNVYNI GTDFEISNLD VAKKIINISI NLNNNNNNNN NNNNNNNNNN
     NNNNNNNNND FNIMDYKKFI NYIDDRPFND HRYNINYSKL SNLGWKKSIS WEEGIEKTFI
     WYKNNRNYWL NLNVDSYENI NNDNNFKYFG NQNNNNENNN NDNNENNNNC RVCSNIDLNY
     GEKKPFHLTL KSEE