TCYC_BACSU
ID TCYC_BACSU Reviewed; 247 AA.
AC P39456;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 16-JUN-2009, sequence version 4.
DT 03-AUG-2022, entry version 136.
DE RecName: Full=L-cystine import ATP-binding protein TcyC;
DE EC=7.4.2.-;
GN Name=tcyC; Synonyms=yckI; OrderedLocusNames=BSU03590;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168 / JH642;
RX PubMed=7551042; DOI=10.1099/13500872-141-7-1781;
RA Rodriguez F., Grandi G.;
RT "An operon encoding a novel ABC-type transport system in Bacillus
RT subtilis.";
RL Microbiology 141:1781-1784(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8407792; DOI=10.1128/jb.175.19.6203-6211.1993;
RA Grossman T.H., Tuckman M., Ellestad S., Osburne M.S.;
RT "Isolation and characterization of Bacillus subtilis genes involved in
RT siderophore biosynthesis: relationship between B. subtilis sfpo and
RT Escherichia coli entD genes.";
RL J. Bacteriol. 175:6203-6211(1993).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RX PubMed=8969502; DOI=10.1099/13500872-142-11-3047;
RA Yamane K., Kumano M., Kurita K.;
RT "The 25 degrees-36 degrees region of the Bacillus subtilis chromosome:
RT determination of the sequence of a 146 kb segment and identification of 113
RT genes.";
RL Microbiology 142:3047-3056(1996).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [5]
RP SEQUENCE REVISION TO 84.
RX PubMed=19383706; DOI=10.1099/mic.0.027839-0;
RA Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A.,
RA Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.;
RT "From a consortium sequence to a unified sequence: the Bacillus subtilis
RT 168 reference genome a decade later.";
RL Microbiology 155:1758-1775(2009).
RN [6]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 66-247.
RC STRAIN=168 / JH642;
RX PubMed=8355609; DOI=10.1111/j.1365-2958.1993.tb01629.x;
RA Cosmina P., Rodriguez F., de Ferra F., Grandi G., Perego M., Venema G.,
RA van Sinderen D.;
RT "Sequence and analysis of the genetic locus responsible for surfactin
RT synthesis in Bacillus subtilis.";
RL Mol. Microbiol. 8:821-831(1993).
RN [7]
RP FUNCTION IN L-CYSTINE TRANSPORT.
RC STRAIN=168;
RX PubMed=15262924; DOI=10.1128/jb.186.15.4875-4884.2004;
RA Burguiere P., Auger S., Hullo M.-F., Danchin A., Martin-Verstraete I.;
RT "Three different systems participate in L-cystine uptake in Bacillus
RT subtilis.";
RL J. Bacteriol. 186:4875-4884(2004).
CC -!- FUNCTION: Part of the ABC transporter complex TcyABC involved in L-
CC cystine import. Responsible for energy coupling to the transport system
CC (Probable). {ECO:0000305|PubMed:15262924}.
CC -!- SUBUNIT: The complex is composed of two ATP-binding proteins (TcyC),
CC two transmembrane proteins (TcyB) and a solute-binding protein (TcyA).
CC {ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Peripheral membrane
CC protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the ABC transporter superfamily. L-cystine
CC importer (TC 3.A.1.3.14) family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; X77636; CAA54726.1; -; Genomic_DNA.
DR EMBL; L17438; AAC36828.1; -; Unassigned_DNA.
DR EMBL; D50453; BAA08992.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB12153.2; -; Genomic_DNA.
DR EMBL; X70356; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR PIR; C69761; C69761.
DR RefSeq; NP_388241.2; NC_000964.3.
DR RefSeq; WP_003246629.1; NZ_JNCM01000031.1.
DR AlphaFoldDB; P39456; -.
DR SMR; P39456; -.
DR STRING; 224308.BSU03590; -.
DR TCDB; 3.A.1.3.14; the atp-binding cassette (abc) superfamily.
DR PaxDb; P39456; -.
DR PRIDE; P39456; -.
DR EnsemblBacteria; CAB12153; CAB12153; BSU_03590.
DR GeneID; 938294; -.
DR KEGG; bsu:BSU03590; -.
DR PATRIC; fig|224308.179.peg.378; -.
DR eggNOG; COG1126; Bacteria.
DR InParanoid; P39456; -.
DR OMA; FQAYHLF; -.
DR PhylomeDB; P39456; -.
DR BioCyc; BSUB:BSU03590-MON; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015424; F:ABC-type amino acid transporter activity; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR030679; ABC_ATPase_HisP-typ.
DR InterPro; IPR003439; ABC_transporter-like_ATP-bd.
DR InterPro; IPR017871; ABC_transporter-like_CS.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF00005; ABC_tran; 1.
DR PIRSF; PIRSF039085; ABC_ATPase_HisP; 1.
DR SMART; SM00382; AAA; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS00211; ABC_TRANSPORTER_1; 1.
DR PROSITE; PS50893; ABC_TRANSPORTER_2; 1.
PE 1: Evidence at protein level;
KW Amino-acid transport; ATP-binding; Cell membrane; Membrane;
KW Nucleotide-binding; Reference proteome; Translocase; Transport.
FT CHAIN 1..247
FT /note="L-cystine import ATP-binding protein TcyC"
FT /id="PRO_0000093138"
FT DOMAIN 2..240
FT /note="ABC transporter"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00434"
FT BINDING 34..41
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00434"
FT CONFLICT 72..93
FT /note="KQADILKLRRKSGMVFQAYHLF -> NRRISKASPKIRNGVSGVSPV (in
FT Ref. 2; AAC36828)"
FT /evidence="ECO:0000305"
FT CONFLICT 84
FT /note="G -> P (in Ref. 3; BAA08992 and 6; X70356)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 247 AA; 27748 MW; CB9CE075CA33C193 CRC64;
MLTVKGLNKS FGENEILKKI DMKIEKGKVI AILGPSGSGK TTLLRCLNAL EIPNRGELAF
DDFSIDFSKK VKQADILKLR RKSGMVFQAY HLFPHRTALE NVMEGPVQVQ KRNKEEVRKE
AIQLLDKVGL KDKMDLYPFQ LSGGQQQRVG IARALAIQPE LMLFDEPTSA LDPELVGEVL
KVIKDLANEG WTMVVVTHEI KFAQEVADEV IFIDGGVIVE QGPPEQIFSA PKEERTQRFL
NRILNPL
