ID   TCYB_BACSU              Reviewed;         234 AA.
AC   P42200;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   03-AUG-2022, entry version 133.
DE   RecName: Full=L-cystine transport system permease protein TcyB;
GN   Name=tcyB; Synonyms=yckJ; OrderedLocusNames=BSU03600;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168 / JH642;
RX   PubMed=7551042; DOI=10.1099/13500872-141-7-1781;
RA   Rodriguez F., Grandi G.;
RT   "An operon encoding a novel ABC-type transport system in Bacillus
RT   subtilis.";
RL   Microbiology 141:1781-1784(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=8969502; DOI=10.1099/13500872-142-11-3047;
RA   Yamane K., Kumano M., Kurita K.;
RT   "The 25 degrees-36 degrees region of the Bacillus subtilis chromosome:
RT   determination of the sequence of a 146 kb segment and identification of 113
RT   genes.";
RL   Microbiology 142:3047-3056(1996).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [4]
RP   FUNCTION IN L-CYSTINE TRANSPORT, AND BIOPHYSICOCHEMICAL PROPERTIES.
RC   STRAIN=168;
RX   PubMed=15262924; DOI=10.1128/jb.186.15.4875-4884.2004;
RA   Burguiere P., Auger S., Hullo M.-F., Danchin A., Martin-Verstraete I.;
RT   "Three different systems participate in L-cystine uptake in Bacillus
RT   subtilis.";
RL   J. Bacteriol. 186:4875-4884(2004).
CC   -!- FUNCTION: Part of the ABC transporter complex TcyABC involved in L-
CC       cystine import. Probably responsible for the translocation of the
CC       substrate across the membrane (Probable).
CC       {ECO:0000305|PubMed:15262924}.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         Vmax=0.35 nmol/min/mg enzyme for L-cystine transport
CC         {ECO:0000269|PubMed:15262924};
CC   -!- SUBUNIT: The complex is composed of two ATP-binding proteins (TcyC),
CC       two transmembrane proteins (TcyB) and a solute-binding protein (TcyA).
CC       {ECO:0000305}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000255|PROSITE-ProRule:PRU00441}.
CC   -!- SIMILARITY: Belongs to the binding-protein-dependent transport system
CC       permease family. {ECO:0000305}.
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DR   EMBL; X77636; CAA54725.1; -; Genomic_DNA.
DR   EMBL; D50453; BAA08993.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB12154.1; -; Genomic_DNA.
DR   PIR; I40451; I40451.
DR   RefSeq; NP_388242.1; NC_000964.3.
DR   RefSeq; WP_003234541.1; NZ_JNCM01000031.1.
DR   AlphaFoldDB; P42200; -.
DR   SMR; P42200; -.
DR   STRING; 224308.BSU03600; -.
DR   TCDB; 3.A.1.3.14; the atp-binding cassette (abc) superfamily.
DR   PaxDb; P42200; -.
DR   EnsemblBacteria; CAB12154; CAB12154; BSU_03600.
DR   GeneID; 938301; -.
DR   KEGG; bsu:BSU03600; -.
DR   PATRIC; fig|224308.179.peg.379; -.
DR   eggNOG; COG0765; Bacteria.
DR   InParanoid; P42200; -.
DR   OMA; QWEAGYS; -.
DR   PhylomeDB; P42200; -.
DR   BioCyc; BSUB:BSU03600-MON; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0043190; C:ATP-binding cassette (ABC) transporter complex; IEA:InterPro.
DR   GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR   GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:0006865; P:amino acid transport; IBA:GO_Central.
DR   CDD; cd06261; TM_PBP2; 1.
DR   Gene3D; 1.10.3720.10; -; 1.
DR   InterPro; IPR010065; AA_ABC_transptr_permease_3TM.
DR   InterPro; IPR043429; ArtM/GltK/GlnP/TcyL/YhdX-like.
DR   InterPro; IPR000515; MetI-like.
DR   InterPro; IPR035906; MetI-like_sf.
DR   PANTHER; PTHR30614; PTHR30614; 1.
DR   Pfam; PF00528; BPD_transp_1; 1.
DR   SUPFAM; SSF161098; SSF161098; 1.
DR   TIGRFAMs; TIGR01726; HEQRo_perm_3TM; 1.
DR   PROSITE; PS50928; ABC_TM1; 1.
PE   1: Evidence at protein level;
KW   Amino-acid transport; Cell membrane; Membrane; Reference proteome;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..234
FT                   /note="L-cystine transport system permease protein TcyB"
FT                   /id="PRO_0000060267"
FT   TRANSMEM        8..28
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT   TRANSMEM        36..56
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT   TRANSMEM        78..98
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT   TRANSMEM        100..120
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT   TRANSMEM        199..219
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT   DOMAIN          32..221
FT                   /note="ABC transmembrane type-1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
SQ   SEQUENCE   234 AA;  26388 MW;  5790DCB40C4721A0 CRC64;
     MFLNNLPALT LGTAIPWDLV QQSFWPILSG GIYYTIPLTI LSFIFGMILA LITALARMSK
     VRPLRWVFSV YVSAIRGTPL LVQLFIIFYL FPAFNVTLDP FPSAVIAFSL NVGAYASEII
     RASILSVPKG QWEAGYTIGM THQKTLFRVI LPQAFRVSIP PLSNTFISLI KDTSLASQIL
     VAELFRKAQE IGARNLDQIL VIYIEAAFIY WIICFLLSLV QHVIERRLDR YVAK