TCYA_BACSU
ID TCYA_BACSU Reviewed; 268 AA.
AC P42199; P94402;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 16-JUN-2009, sequence version 3.
DT 03-AUG-2022, entry version 128.
DE RecName: Full=L-cystine-binding protein TcyA;
DE Flags: Precursor;
GN Name=tcyA; Synonyms=yckK; OrderedLocusNames=BSU03610;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168 / JH642;
RX PubMed=7551042; DOI=10.1099/13500872-141-7-1781;
RA Rodriguez F., Grandi G.;
RT "An operon encoding a novel ABC-type transport system in Bacillus
RT subtilis.";
RL Microbiology 141:1781-1784(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RX PubMed=8969502; DOI=10.1099/13500872-142-11-3047;
RA Yamane K., Kumano M., Kurita K.;
RT "The 25 degrees-36 degrees region of the Bacillus subtilis chromosome:
RT determination of the sequence of a 146 kb segment and identification of 113
RT genes.";
RL Microbiology 142:3047-3056(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [4]
RP SEQUENCE REVISION TO 95 AND 190.
RX PubMed=19383706; DOI=10.1099/mic.0.027839-0;
RA Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A.,
RA Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.;
RT "From a consortium sequence to a unified sequence: the Bacillus subtilis
RT 168 reference genome a decade later.";
RL Microbiology 155:1758-1775(2009).
RN [5]
RP FUNCTION IN L-CYSTINE TRANSPORT.
RC STRAIN=168;
RX PubMed=15262924; DOI=10.1128/jb.186.15.4875-4884.2004;
RA Burguiere P., Auger S., Hullo M.-F., Danchin A., Martin-Verstraete I.;
RT "Three different systems participate in L-cystine uptake in Bacillus
RT subtilis.";
RL J. Bacteriol. 186:4875-4884(2004).
CC -!- FUNCTION: Part of the ABC transporter complex TcyABC involved in L-
CC cystine import. {ECO:0000269|PubMed:15262924}.
CC -!- SUBUNIT: The complex is composed of two ATP-binding proteins (TcyC),
CC two transmembrane proteins (TcyB) and a solute-binding protein (TcyA).
CC {ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Lipid-anchor
CC {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the bacterial solute-binding protein 3 family.
CC {ECO:0000305}.
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DR EMBL; X77636; CAA54724.1; -; Genomic_DNA.
DR EMBL; D50453; BAA08994.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB12155.2; -; Genomic_DNA.
DR PIR; E69761; E69761.
DR RefSeq; NP_388243.2; NC_000964.3.
DR RefSeq; WP_003246699.1; NZ_JNCM01000031.1.
DR AlphaFoldDB; P42199; -.
DR SMR; P42199; -.
DR STRING; 224308.BSU03610; -.
DR TCDB; 3.A.1.3.14; the atp-binding cassette (abc) superfamily.
DR jPOST; P42199; -.
DR PaxDb; P42199; -.
DR PRIDE; P42199; -.
DR EnsemblBacteria; CAB12155; CAB12155; BSU_03610.
DR GeneID; 938298; -.
DR KEGG; bsu:BSU03610; -.
DR PATRIC; fig|224308.179.peg.380; -.
DR eggNOG; COG0834; Bacteria.
DR InParanoid; P42199; -.
DR OMA; WFGIDVS; -.
DR PhylomeDB; P42199; -.
DR BioCyc; BSUB:BSU03610-MON; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0006865; P:amino acid transport; IEA:UniProtKB-KW.
DR InterPro; IPR018313; SBP_3_CS.
DR InterPro; IPR001638; Solute-binding_3/MltF_N.
DR Pfam; PF00497; SBP_bac_3; 1.
DR SMART; SM00062; PBPb; 1.
DR PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
DR PROSITE; PS01039; SBP_BACTERIAL_3; 1.
PE 1: Evidence at protein level;
KW Amino-acid transport; Cell membrane; Lipoprotein; Membrane; Palmitate;
KW Reference proteome; Signal; Transport.
FT SIGNAL 1..19
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT CHAIN 20..268
FT /note="L-cystine-binding protein TcyA"
FT /id="PRO_0000031778"
FT LIPID 20
FT /note="N-palmitoyl cysteine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT LIPID 20
FT /note="S-diacylglycerol cysteine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT CONFLICT 81..82
FT /note="KR -> NS (in Ref. 1; CAA54724)"
FT /evidence="ECO:0000305"
FT CONFLICT 95
FT /note="D -> G (in Ref. 2; BAA08994)"
FT /evidence="ECO:0000305"
FT CONFLICT 120..121
FT /note="DK -> NQ (in Ref. 1; CAA54724)"
FT /evidence="ECO:0000305"
FT CONFLICT 190
FT /note="G -> A (in Ref. 2; BAA08994)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 268 AA; 29514 MW; E88B56676E8AD762 CRC64;
MKKALLALFM VVSIAALAAC GAGNDNQSKD NAKDGDLWAS IKKKGVLTVG TEGTYEPFTY
HDKDTDKLTG YDVEVITEVA KRLGLKVDFK ETQWDSMFAG LNSKRFDVVA NQVGKTDRED
KYDFSDKYTT SRAVVVTKKD NNDIKSEADV KGKTSAQSLT SNYNKLATNA GAKVEGVEGM
AQALQMIQQG RVDMTYNDKL AVLNYLKTSG NKNVKIAFET GEPQSTYFTF RKGSGEVVDQ
VNKALKEMKE DGTLSKISKK WFGEDVSK
