T2FB_DROME
ID T2FB_DROME Reviewed; 277 AA.
AC P41900; Q9VGX4;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 2.
DT 03-AUG-2022, entry version 164.
DE RecName: Full=General transcription factor IIF subunit 2;
DE AltName: Full=Transcription initiation factor IIF subunit beta;
DE Short=TFIIF-beta;
GN Name=TfIIFbeta; Synonyms=TFIIF30; ORFNames=CG6538;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=7596813; DOI=10.1093/nar/23.11.1882;
RA Gong D.-W., Mortin M.A., Horikoshi M., Nakatani Y.;
RT "Molecular cloning of cDNA encoding the small subunit of Drosophila
RT transcription initiation factor TFIIF.";
RL Nucleic Acids Res. 23:1882-1886(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], AND FUNCTION.
RC STRAIN=Canton-S;
RX PubMed=7890767; DOI=10.1074/jbc.270.11.6292;
RA Frank D.J., Tyree C.M., George C.P., Kadonaga J.T.;
RT "Structure and function of the small subunit of TFIIF (RAP30) from
RT Drosophila melanogaster.";
RL J. Biol. Chem. 270:6292-6297(1995).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
CC -!- FUNCTION: TFIIF is a general transcription initiation factor that binds
CC to RNA polymerase II and helps to recruit it to the initiation complex
CC in collaboration with TFIIB. {ECO:0000269|PubMed:7890767}.
CC -!- SUBUNIT: Heterodimer of an alpha and a beta subunit.
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- SIMILARITY: Belongs to the TFIIF beta subunit family. {ECO:0000305}.
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DR EMBL; U25188; AAA86888.1; -; mRNA.
DR EMBL; U02461; AAA81888.1; -; mRNA.
DR EMBL; AE014297; AAF54548.1; -; Genomic_DNA.
DR EMBL; AY071514; AAL49136.1; -; mRNA.
DR PIR; S57336; S57336.
DR RefSeq; NP_524305.2; NM_079581.4.
DR AlphaFoldDB; P41900; -.
DR SMR; P41900; -.
DR BioGRID; 66434; 11.
DR IntAct; P41900; 2.
DR STRING; 7227.FBpp0081786; -.
DR PaxDb; P41900; -.
DR PRIDE; P41900; -.
DR DNASU; 41290; -.
DR EnsemblMetazoa; FBtr0082310; FBpp0081786; FBgn0010421.
DR GeneID; 41290; -.
DR KEGG; dme:Dmel_CG6538; -.
DR CTD; 41290; -.
DR FlyBase; FBgn0010421; TfIIFbeta.
DR VEuPathDB; VectorBase:FBgn0010421; -.
DR eggNOG; KOG2905; Eukaryota.
DR GeneTree; ENSGT00390000016051; -.
DR HOGENOM; CLU_047858_1_0_1; -.
DR InParanoid; P41900; -.
DR OMA; KYIANKW; -.
DR OrthoDB; 1496393at2759; -.
DR PhylomeDB; P41900; -.
DR Reactome; R-DME-112382; Formation of RNA Pol II elongation complex.
DR Reactome; R-DME-113418; Formation of the Early Elongation Complex.
DR Reactome; R-DME-674695; RNA Polymerase II Pre-transcription Events.
DR Reactome; R-DME-6796648; TP53 Regulates Transcription of DNA Repair Genes.
DR Reactome; R-DME-6803529; FGFR2 alternative splicing.
DR Reactome; R-DME-6807505; RNA polymerase II transcribes snRNA genes.
DR Reactome; R-DME-72086; mRNA Capping.
DR Reactome; R-DME-72163; mRNA Splicing - Major Pathway.
DR Reactome; R-DME-72165; mRNA Splicing - Minor Pathway.
DR Reactome; R-DME-72203; Processing of Capped Intron-Containing Pre-mRNA.
DR Reactome; R-DME-73776; RNA Polymerase II Promoter Escape.
DR Reactome; R-DME-73779; RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
DR Reactome; R-DME-75953; RNA Polymerase II Transcription Initiation.
DR Reactome; R-DME-75955; RNA Polymerase II Transcription Elongation.
DR Reactome; R-DME-76042; RNA Polymerase II Transcription Initiation And Promoter Clearance.
DR Reactome; R-DME-77075; RNA Pol II CTD phosphorylation and interaction with CE.
DR Reactome; R-DME-9018519; Estrogen-dependent gene expression.
DR SignaLink; P41900; -.
DR BioGRID-ORCS; 41290; 1 hit in 1 CRISPR screen.
DR GenomeRNAi; 41290; -.
DR PRO; PR:P41900; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0010421; Expressed in egg chamber and 25 other tissues.
DR Genevisible; P41900; DM.
DR GO; GO:0005634; C:nucleus; IDA:FlyBase.
DR GO; GO:0005674; C:transcription factor TFIIF complex; IDA:FlyBase.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003678; F:DNA helicase activity; IEA:UniProtKB-EC.
DR GO; GO:0001096; F:TFIIF-class transcription factor complex binding; IPI:FlyBase.
DR GO; GO:0006366; P:transcription by RNA polymerase II; IDA:FlyBase.
DR GO; GO:0006368; P:transcription elongation from RNA polymerase II promoter; IDA:FlyBase.
DR GO; GO:0006367; P:transcription initiation from RNA polymerase II promoter; IDA:FlyBase.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR003196; TFIIF_beta.
DR InterPro; IPR040450; TFIIF_beta_HTH.
DR InterPro; IPR040504; TFIIF_beta_N.
DR InterPro; IPR011039; TFIIF_interaction.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR PANTHER; PTHR10445; PTHR10445; 1.
DR Pfam; PF02270; TFIIF_beta; 1.
DR Pfam; PF17683; TFIIF_beta_N; 1.
DR PIRSF; PIRSF015849; TFIIF-beta; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
DR SUPFAM; SSF50916; SSF50916; 1.
PE 2: Evidence at transcript level;
KW DNA-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..277
FT /note="General transcription factor IIF subunit 2"
FT /id="PRO_0000211239"
FT CONFLICT 97..117
FT /note="VFSHMAPSDGKENSTTSAAQP -> YSRTWHRPMARRTRLPRRHS (in
FT Ref. 2; AAA81888)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 277 AA; 32107 MW; ED128DE2815D21CA CRC64;
MSKEDKEKTQ IIDKDLDLSN AGRGVWLVKV PKYIAQKWEK APTNMDVGKL RINKTPGQKA
QVSLSLTPAV LALDPEEKIP TEHILDVSQV TKQTLGVFSH MAPSDGKENS TTSAAQPDNE
KLYMEGRIVQ KLECRPIADN CYMKLKLESI RKASEPQRRV QPIDKIVQNF KPVKDHAHNI
EYRERKKAEG KKARDDKNAV MDMLFHAFEK HQYYNIKDLV KITNQPISYL KEILKDVCDY
NMKNPHKNMW ELKKEYRHYK TEEKKEEEHK SGSSDSE
