ID   SYL_METMP               Reviewed;         955 AA.
AC   Q6LZD2;
DT   01-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 110.
DE   RecName: Full=Leucine--tRNA ligase {ECO:0000255|HAMAP-Rule:MF_00049};
DE            EC=6.1.1.4 {ECO:0000255|HAMAP-Rule:MF_00049};
DE   AltName: Full=Leucyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00049};
DE            Short=LeuRS {ECO:0000255|HAMAP-Rule:MF_00049};
GN   Name=leuS {ECO:0000255|HAMAP-Rule:MF_00049}; OrderedLocusNames=MMP0697;
OS   Methanococcus maripaludis (strain S2 / LL).
OC   Archaea; Euryarchaeota; Methanomada group; Methanococci; Methanococcales;
OC   Methanococcaceae; Methanococcus.
OX   NCBI_TaxID=267377;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=S2 / LL;
RX   PubMed=15466049; DOI=10.1128/jb.186.20.6956-6969.2004;
RA   Hendrickson E.L., Kaul R., Zhou Y., Bovee D., Chapman P., Chung J.,
RA   Conway de Macario E., Dodsworth J.A., Gillett W., Graham D.E., Hackett M.,
RA   Haydock A.K., Kang A., Land M.L., Levy R., Lie T.J., Major T.A.,
RA   Moore B.C., Porat I., Palmeiri A., Rouse G., Saenphimmachak C., Soell D.,
RA   Van Dien S., Wang T., Whitman W.B., Xia Q., Zhang Y., Larimer F.W.,
RA   Olson M.V., Leigh J.A.;
RT   "Complete genome sequence of the genetically tractable hydrogenotrophic
RT   methanogen Methanococcus maripaludis.";
RL   J. Bacteriol. 186:6956-6969(2004).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-
CC         tRNA(Leu); Xref=Rhea:RHEA:11688, Rhea:RHEA-COMP:9613, Rhea:RHEA-
CC         COMP:9622, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57427,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78494, ChEBI:CHEBI:456215; EC=6.1.1.4;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00049};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00049}.
CC   -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00049}.
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DR   EMBL; BX950229; CAF30253.1; -; Genomic_DNA.
DR   RefSeq; WP_011170641.1; NC_005791.1.
DR   AlphaFoldDB; Q6LZD2; -.
DR   SMR; Q6LZD2; -.
DR   STRING; 267377.MMP0697; -.
DR   EnsemblBacteria; CAF30253; CAF30253; MMP0697.
DR   GeneID; 2761186; -.
DR   KEGG; mmp:MMP0697; -.
DR   PATRIC; fig|267377.15.peg.714; -.
DR   eggNOG; arCOG00809; Archaea.
DR   HOGENOM; CLU_004174_0_0_2; -.
DR   OMA; AWNMAFQ; -.
DR   OrthoDB; 4914at2157; -.
DR   BioCyc; MMAR267377:MMP_RS03650-MON; -.
DR   Proteomes; UP000000590; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004823; F:leucine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006429; P:leucyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.50.620; -; 1.
DR   Gene3D; 3.90.740.10; -; 1.
DR   HAMAP; MF_00049_A; Leu_tRNA_synth_A; 1.
DR   InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR   InterPro; IPR002300; aa-tRNA-synth_Ia.
DR   InterPro; IPR020791; Leu-tRNA-lgase_arc.
DR   InterPro; IPR004493; Leu-tRNA-synth_Ia_arc/euk.
DR   InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR   InterPro; IPR015413; Methionyl/Leucyl_tRNA_Synth.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR   InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR   PANTHER; PTHR45794; PTHR45794; 2.
DR   Pfam; PF08264; Anticodon_1; 1.
DR   Pfam; PF00133; tRNA-synt_1; 1.
DR   Pfam; PF09334; tRNA-synt_1g; 1.
DR   SUPFAM; SSF47323; SSF47323; 1.
DR   SUPFAM; SSF50677; SSF50677; 1.
DR   TIGRFAMs; TIGR00395; leuS_arch; 1.
DR   PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..955
FT                   /note="Leucine--tRNA ligase"
FT                   /id="PRO_0000152134"
FT   MOTIF           51..61
FT                   /note="'HIGH' region"
FT   MOTIF           647..651
FT                   /note="'KMSKS' region"
FT   BINDING         650
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00049"
SQ   SEQUENCE   955 AA;  110690 MW;  E0C03FBAA1C7E93F CRC64;
     MDQKEVIGET GHKSVDLIKI MDKWQKKWTE AKIFEAEHDS RDKFFITAAF PYLNGVLHAG
     HLRTFTIPET IARYQRMKNK NVLWTFGFHV TGTPILGLAN QIKERKEDII WAYNNLHNIP
     MDELLKLNTP EAIVECFSKK ATEAFKRMGF SLDWRRNFKT DDKVFSKFIE WQFYKLKEMG
     HITKGSHPVR YCPKCENPVE DHDLLHGEES TTVEYSLIKF TSKFDGKEII MPMATLRPET
     VFGVTNAWVN PNEIYVMAEV HDEIQKLDSE DVDLKYNGIW IIGKECADKL KEQDRKIEIL
     KEIKGSELLG LKIKNPVTKK EVPLLPADFV EMGIGTGCVM SVPAHAPYDY VALRDLGKVE
     EVGLIPLIEI EGYDKYPAKE IVEKLGIKDQ NDEELLEQAT SKIYKDEFHK GKLNENCGEY
     TGISVKDIKE KLTKDYINSN IAEIMYEFSE QKVVCRCGEK CIIKTVKGQW FINYSDENWK
     KLAHECIDNM NFAPEGIRQE FHNKVDWMKD KACARKRGLG TILPFDENWI IESLSDSTIY
     MAYYTIARFI NEGLTPEQLI PELFDYVYLG NGNAEEIAKN SKIQAETIEE MRKEFLYYYP
     LDWRCSAKDL IPNHLTFMIF NHVALFGREH WPRGIEINGY VTIEGKKLSK SKGPVLPVSE
     VAENFGADVA RFYITTCAEL PQDADVKFKE MEKARDNLIK LYDLAVSVME EESAEKELSL
     IDKWLLHKTY SSINSAETAY EEFQLRKIGL MFYELINDLR WYKRRGGENN SVLKEVVEIW
     TKLLSPVTPH LCEEIWEKLG YAGFISQEMY PVSKPELINE DLELGEEFIK SAMEDIRNIK
     GVAKINPEKM YLYTADDWKY DLLEFMNENA EKNVKALIPM VMKEDKFKRH GKEVMKLINE
     IMKIGVKKAI AEVEILENAK TFIESEFDCK VIVNGEDVKG KKKFAIPYKP AIYME