ID   SYL_ACET2               Reviewed;         825 AA.
AC   A3DEU0;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   20-MAR-2007, sequence version 1.
DT   03-AUG-2022, entry version 99.
DE   RecName: Full=Leucine--tRNA ligase {ECO:0000255|HAMAP-Rule:MF_00049};
DE            EC=6.1.1.4 {ECO:0000255|HAMAP-Rule:MF_00049};
DE   AltName: Full=Leucyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00049};
DE            Short=LeuRS {ECO:0000255|HAMAP-Rule:MF_00049};
GN   Name=leuS {ECO:0000255|HAMAP-Rule:MF_00049}; OrderedLocusNames=Cthe_1237;
OS   Acetivibrio thermocellus (strain ATCC 27405 / DSM 1237 / JCM 9322 / NBRC
OS   103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Clostridium thermocellum).
OC   Bacteria; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae;
OC   Acetivibrio.
OX   NCBI_TaxID=203119;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 27405 / DSM 1237 / JCM 9322 / NBRC 103400 / NCIMB 10682 / NRRL
RC   B-4536 / VPI 7372;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S.,
RA   Chertkov O., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J.,
RA   Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Wu J.H.D.,
RA   Newcomb M., Richardson P.;
RT   "Complete sequence of Clostridium thermocellum ATCC 27405.";
RL   Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-
CC         tRNA(Leu); Xref=Rhea:RHEA:11688, Rhea:RHEA-COMP:9613, Rhea:RHEA-
CC         COMP:9622, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57427,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78494, ChEBI:CHEBI:456215; EC=6.1.1.4;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00049};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00049}.
CC   -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00049}.
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DR   EMBL; CP000568; ABN52469.1; -; Genomic_DNA.
DR   RefSeq; WP_003517434.1; NC_009012.1.
DR   AlphaFoldDB; A3DEU0; -.
DR   SMR; A3DEU0; -.
DR   STRING; 203119.Cthe_1237; -.
DR   EnsemblBacteria; ABN52469; ABN52469; Cthe_1237.
DR   KEGG; cth:Cthe_1237; -.
DR   eggNOG; COG0495; Bacteria.
DR   HOGENOM; CLU_004427_0_0_9; -.
DR   OMA; TFMVLAP; -.
DR   OrthoDB; 32262at2; -.
DR   Proteomes; UP000002145; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004823; F:leucine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006429; P:leucyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.50.620; -; 2.
DR   HAMAP; MF_00049_B; Leu_tRNA_synth_B; 1.
DR   InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR   InterPro; IPR002300; aa-tRNA-synth_Ia.
DR   InterPro; IPR002302; Leu-tRNA-ligase.
DR   InterPro; IPR025709; Leu_tRNA-synth_edit.
DR   InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR   InterPro; IPR015413; Methionyl/Leucyl_tRNA_Synth.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR   InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR   PANTHER; PTHR43740; PTHR43740; 2.
DR   Pfam; PF08264; Anticodon_1; 1.
DR   Pfam; PF00133; tRNA-synt_1; 1.
DR   Pfam; PF13603; tRNA-synt_1_2; 1.
DR   Pfam; PF09334; tRNA-synt_1g; 1.
DR   PRINTS; PR00985; TRNASYNTHLEU.
DR   SUPFAM; SSF47323; SSF47323; 1.
DR   SUPFAM; SSF50677; SSF50677; 1.
DR   TIGRFAMs; TIGR00396; leuS_bact; 1.
DR   PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..825
FT                   /note="Leucine--tRNA ligase"
FT                   /id="PRO_1000009331"
FT   MOTIF           40..50
FT                   /note="'HIGH' region"
FT   MOTIF           580..584
FT                   /note="'KMSKS' region"
FT   BINDING         583
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00049"
SQ   SEQUENCE   825 AA;  94886 MW;  45416BD31E0D0AEC CRC64;
     MYYNFVDIEK KWQKKWLEEK AFAVREDESK KKYYVLEMFP YPSGNLHMGH VRNYSIGDVV
     ARFKRMNGFN VLHPMGWDAF GLPAENAAIK RGVHPNDWTW SNIDNMRRQL KQLGISYDWD
     REVATCHPDY YKWTQWMFLQ LYKNGLAYKK KAYVNWCPSC ATVLANEQVV NGVCERCKSV
     VGKKDLEQWF FKITDYAQRL LDDIEKLKGW PDKVKVMQQN WIGRSEGVEV DFKVDGMDKA
     VRVYTTRPDT IYGVTYVVIA PEHPVVKELI KGTEQEQVCN EFINKMMFLN EIDRTATDVE
     KEGVFTGRYV INPLNGDRVP LYLANYVLAE YGTGVVMAVP AHDQRDFEFA KKYNLPIKVV
     IQPEGQELDA SRMTEAFVEV GYLVNSAEFD GVRSDEAIGK IIDYIEQKGY GKRKINYRLR
     DWLISRQRYW GAPIPIIYCD DCGAVPVPEE DLPVILPTDI KFSGVGESPL STSETFISAP
     CPKCGKMGRR ELDTMDTFVC SSWYYLRYCD PCNDKAPFDK ERIRYWLPVD QYIGGVEHAI
     LHLLYSRFLM KVLYDLGYVD YDEPFTNLLT QGMVLKDGAK MSKSLGNVVS PEEIIEKYGA
     DTARLFILFA SPPEKDLEWS DQGVEGCYRF INRVWRIVNE FADAVKEGGN IDTSTFTKAD
     KELWYMLNNT LKRVTDDISQ RFNFNTAISA VMELVNSLYY YKDKVADDSK NKALVREVIE
     KLIIMLAPFI PHATEELWSA IGKEGSVHEQ KWPSFDPAAL VKDEIEIVVQ INGKVRDKIV
     VPSDLTKEQV EERALNSEKI KAETAGKNVV KVISVPGKLV NIVVK