ID   BLE1_PSEAI              Reviewed;         308 AA.
AC   P37321;
DT   01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1994, sequence version 1.
DT   03-AUG-2022, entry version 94.
DE   RecName: Full=Extended-spectrum beta-lactamase PER-1;
DE            EC=3.5.2.6;
DE   Flags: Precursor;
GN   Name=per1;
OS   Pseudomonas aeruginosa.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=287;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PARTIAL PROTEIN SEQUENCE.
RC   STRAIN=RNL-1;
RX   PubMed=8141562; DOI=10.1128/aac.38.1.104;
RA   Nordmann P., Naas T.;
RT   "Sequence analysis of PER-1 extended-spectrum beta-lactamase from
RT   Pseudomonas aeruginosa and comparison with class A beta-lactamases.";
RL   Antimicrob. Agents Chemother. 38:104-114(1994).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a beta-lactam + H2O = a substituted beta-amino acid;
CC         Xref=Rhea:RHEA:20401, ChEBI:CHEBI:15377, ChEBI:CHEBI:35627,
CC         ChEBI:CHEBI:140347; EC=3.5.2.6; Evidence={ECO:0000255|PROSITE-
CC         ProRule:PRU10101};
CC   -!- SIMILARITY: Belongs to the class-A beta-lactamase family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; Z21957; CAA79968.1; -; Genomic_DNA.
DR   PIR; S35931; S35931.
DR   RefSeq; WP_001100753.1; NZ_RXTL01000078.1.
DR   PDB; 1E25; X-ray; 1.90 A; A=27-308.
DR   PDBsum; 1E25; -.
DR   AlphaFoldDB; P37321; -.
DR   SMR; P37321; -.
DR   DrugBank; DB09060; Avibactam.
DR   KEGG; ag:CAA79968; -.
DR   SABIO-RK; P37321; -.
DR   EvolutionaryTrace; P37321; -.
DR   GO; GO:0008800; F:beta-lactamase activity; IEA:UniProtKB-EC.
DR   GO; GO:0030655; P:beta-lactam antibiotic catabolic process; IEA:InterPro.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.710.10; -; 1.
DR   InterPro; IPR012338; Beta-lactam/transpept-like.
DR   InterPro; IPR045155; Beta-lactam_cat.
DR   InterPro; IPR000871; Beta-lactam_class-A.
DR   InterPro; IPR023650; Beta-lactam_class-A_AS.
DR   PANTHER; PTHR35333; PTHR35333; 1.
DR   Pfam; PF13354; Beta-lactamase2; 1.
DR   PRINTS; PR00118; BLACTAMASEA.
DR   SUPFAM; SSF56601; SSF56601; 1.
DR   PROSITE; PS00146; BETA_LACTAMASE_A; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Antibiotic resistance; Direct protein sequencing; Hydrolase;
KW   Signal.
FT   SIGNAL          1..26
FT   CHAIN           27..308
FT                   /note="Extended-spectrum beta-lactamase PER-1"
FT                   /id="PRO_0000017042"
FT   ACT_SITE        71
FT                   /note="Acyl-ester intermediate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10101"
FT   BINDING         239..241
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   HELIX           29..39
FT                   /evidence="ECO:0007829|PDB:1E25"
FT   STRAND          42..51
FT                   /evidence="ECO:0007829|PDB:1E25"
FT   STRAND          59..62
FT                   /evidence="ECO:0007829|PDB:1E25"
FT   HELIX           70..72
FT                   /evidence="ECO:0007829|PDB:1E25"
FT   HELIX           73..86
FT                   /evidence="ECO:0007829|PDB:1E25"
FT   STRAND          95..99
FT                   /evidence="ECO:0007829|PDB:1E25"
FT   TURN            100..102
FT                   /evidence="ECO:0007829|PDB:1E25"
FT   HELIX           111..114
FT                   /evidence="ECO:0007829|PDB:1E25"
FT   STRAND          117..123
FT                   /evidence="ECO:0007829|PDB:1E25"
FT   HELIX           124..134
FT                   /evidence="ECO:0007829|PDB:1E25"
FT   HELIX           137..147
FT                   /evidence="ECO:0007829|PDB:1E25"
FT   HELIX           149..159
FT                   /evidence="ECO:0007829|PDB:1E25"
FT   HELIX           171..176
FT                   /evidence="ECO:0007829|PDB:1E25"
FT   HELIX           180..183
FT                   /evidence="ECO:0007829|PDB:1E25"
FT   STRAND          184..186
FT                   /evidence="ECO:0007829|PDB:1E25"
FT   HELIX           188..199
FT                   /evidence="ECO:0007829|PDB:1E25"
FT   HELIX           206..217
FT                   /evidence="ECO:0007829|PDB:1E25"
FT   TURN            226..229
FT                   /evidence="ECO:0007829|PDB:1E25"
FT   STRAND          236..242
FT                   /evidence="ECO:0007829|PDB:1E25"
FT   STRAND          249..260
FT                   /evidence="ECO:0007829|PDB:1E25"
FT   STRAND          266..276
FT                   /evidence="ECO:0007829|PDB:1E25"
FT   HELIX           280..304
FT                   /evidence="ECO:0007829|PDB:1E25"
SQ   SEQUENCE   308 AA;  33573 MW;  CED838DFC7D6E117 CRC64;
     MNVIIKAVVT ASTLLMVSFS SFETSAQSPL LKEQIESIVI GKKATVGVAV WGPDDLEPLL
     INPFEKFPMQ SVFKLHLAML VLHQVDQGKL DLNQTVIVNR AKVLQNTWAP IMKAYQGDEF
     SVPVQQLLQY SVSHSDNVAC DLLFELVGGP AALHDYIQSM GIKETAVVAN EAQMHADDQV
     QYQNWTSMKG AAEILKKFEQ KTQLSETSQA LLWKWMVETT TGPERLKGLL PAGTVVAHKT
     GTSGIKAGKT AATNDLGIIL LPDGRPLLVA VFVKDSAESS RTNEAIIAQV AQTAYQFELK
     KLSALSPN