ID   SYGB_LACLA              Reviewed;         673 AA.
AC   Q9CGK0;
DT   08-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2001, sequence version 1.
DT   25-MAY-2022, entry version 103.
DE   RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            EC=6.1.1.14 {ECO:0000255|HAMAP-Rule:MF_00255};
DE   AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            Short=GlyRS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   Name=glyS {ECO:0000255|HAMAP-Rule:MF_00255}; Synonyms=glyT;
GN   OrderedLocusNames=LL1096; ORFNames=L0360;
OS   Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC   Lactococcus.
OX   NCBI_TaxID=272623;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=IL1403;
RX   PubMed=11337471; DOI=10.1101/gr.gr-1697r;
RA   Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J.,
RA   Ehrlich S.D., Sorokin A.;
RT   "The complete genome sequence of the lactic acid bacterium Lactococcus
RT   lactis ssp. lactis IL1403.";
RL   Genome Res. 11:731-753(2001).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC         tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC         COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC         EC=6.1.1.14; Evidence={ECO:0000255|HAMAP-Rule:MF_00255};
CC   -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
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DR   EMBL; AE005176; AAK05194.1; -; Genomic_DNA.
DR   PIR; H86761; H86761.
DR   RefSeq; NP_267252.1; NC_002662.1.
DR   RefSeq; WP_003130669.1; NC_002662.1.
DR   AlphaFoldDB; Q9CGK0; -.
DR   SMR; Q9CGK0; -.
DR   STRING; 272623.L0360; -.
DR   PaxDb; Q9CGK0; -.
DR   EnsemblBacteria; AAK05194; AAK05194; L0360.
DR   KEGG; lla:L0360; -.
DR   PATRIC; fig|272623.7.peg.1174; -.
DR   eggNOG; COG0751; Bacteria.
DR   HOGENOM; CLU_007220_2_2_9; -.
DR   OMA; LPIPKRM; -.
DR   Proteomes; UP000002196; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR   InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR   InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR   PANTHER; PTHR30075; PTHR30075; 1.
DR   Pfam; PF02092; tRNA_synt_2f; 1.
DR   PRINTS; PR01045; TRNASYNTHGB.
DR   TIGRFAMs; TIGR00211; glyS; 1.
DR   PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..673
FT                   /note="Glycine--tRNA ligase beta subunit"
FT                   /id="PRO_0000072908"
SQ   SEQUENCE   673 AA;  75717 MW;  99CF078804DAE69E CRC64;
     MTNYLLEIGL EEIPAHLVTP SINQLAERME TFLKENRLEF DKIIKFSTPR RLAIIVEGLA
     EASEAIDEEV KGPSAKIAKD AQGNWSKAIQ GFSRGQGATP DDLILKGDYY YAKKHIDGVK
     AEEILSKVGD EVIAKMTFST YMKWGNNDFL FVRPIQWIVS LLEDKVVAFD LLDVTANRFS
     RGHRFLANVE VELKNANDYA SKMPENFVLV DAEHRKAEIS AQILALASEN KWQVTLHKDL
     LEEVNNIVEY PTAFVGSFDP KYLSVPAEVL VTSMRDNQRY FEVYNQEGQL APNFISVRNG
     NAENIENVVL GNEKVLVARL EDAEFFWKED QKLKIEDLVA KLAKVTFHAK IGSITEHMAR
     TKEIAAKLAD IAGLTDEEKR DVARSAEIYK FDLLTGMVGE FDELQGVMGE KYALLAGENA
     NVSAAIREHY MPTSAEGELP ETKVGSVLAA ADKIDSVLSF FNVGLIPSGS NDPYALRRAV
     QGLIRIIEKM NWHFDLSLFI DQFEGENHLE ILDFVKARIQ KLLLEKLDRH DIVEAAINSS
     NFDITNMMES AFVIDGHKLH EPFKPAIENV SRSINLVKKA KDIKEINPTL FEEDAEEALY
     NVVISLQNQW TYMPGEEKFR AIVHSLAPAI ETFFESVMVM AEDLSVRDNR IALLSEVVAL
     TSVMADFSLI NTK