STPG4_MOUSE
ID STPG4_MOUSE Reviewed; 247 AA.
AC Q9DAG5;
DT 13-NOV-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 106.
DE RecName: Full=Protein STPG4 {ECO:0000305};
DE AltName: Full=Gonad-specific expression gene protein {ECO:0000303|PubMed:16571911};
DE Short=GSE {ECO:0000303|PubMed:16571911};
DE AltName: Full=Sperm-tail PG-rich repeat-containing protein 4 {ECO:0000312|MGI:MGI:1922717};
GN Name=Stpg4 {ECO:0000312|MGI:MGI:1922717};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=16571911; DOI=10.1262/jrd.18005;
RA Mizuno S., Sono Y., Matsuoka T., Matsumoto K., Saeki K., Hosoi Y.,
RA Fukuda A., Morimoto Y., Iritani A.;
RT "Expression and subcellular localization of GSE protein in germ cells and
RT preimplantation embryos.";
RL J. Reprod. Dev. 52:429-438(2006).
RN [3]
RP FUNCTION, INTERACTION WITH HISTONE H3 AND HISTONE H4, CHROMATIN BINDING,
RP SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=23560077; DOI=10.1371/journal.pone.0060205;
RA Hatanaka Y., Shimizu N., Nishikawa S., Tokoro M., Shin S.W., Nishihara T.,
RA Amano T., Anzai M., Kato H., Mitani T., Hosoi Y., Kishigami S.,
RA Matsumoto K.;
RT "GSE is a maternal factor involved in active DNA demethylation in
RT zygotes.";
RL PLoS ONE 8:E60205-E60205(2013).
RN [4]
RP FUNCTION, INTERACTION WITH METTL23, AND DISRUPTION PHENOTYPE.
RX PubMed=28930672; DOI=10.1016/j.celrep.2017.08.088;
RA Hatanaka Y., Tsusaka T., Shimizu N., Morita K., Suzuki T., Machida S.,
RA Satoh M., Honda A., Hirose M., Kamimura S., Ogonuki N., Nakamura T.,
RA Inoue K., Hosoi Y., Dohmae N., Nakano T., Kurumizaka H., Matsumoto K.,
RA Shinkai Y., Ogura A.;
RT "Histone H3 methylated at arginine 17 is essential for reprogramming the
RT paternal genome in zygotes.";
RL Cell Rep. 20:2756-2765(2017).
CC -!- FUNCTION: Maternal factor that plays a role in epigenetic chromatin
CC reprogramming during early development of the zygote (PubMed:23560077,
CC PubMed:28930672). Involved in the regulation of gametic DNA
CC demethylation by inducing the conversion of the modified genomic base
CC 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC)
CC (PubMed:23560077, PubMed:28930672). {ECO:0000269|PubMed:23560077,
CC ECO:0000269|PubMed:28930672}.
CC -!- SUBUNIT: Interacts with histone H3 (PubMed:23560077). Interacts with
CC histone H4 (PubMed:23560077). Interacts with METTL23 (PubMed:28930672).
CC {ECO:0000269|PubMed:23560077, ECO:0000269|PubMed:28930672}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:16571911}. Nucleus
CC {ECO:0000269|PubMed:16571911, ECO:0000269|PubMed:23560077}.
CC Note=Localizes in female and male zygote pronucleus (PubMed:23560077,
CC PubMed:16571911). Associates preferentially with the paternal chromatin
CC during zygote development (PubMed:23560077).
CC {ECO:0000269|PubMed:23560077}.
CC -!- TISSUE SPECIFICITY: Expressed in ovary and testis (PubMed:16571911).
CC Expressed in spermatocytes I in the pachytene stage, round spermatids,
CC and elongated spermatids (PubMed:16571911). Expressed in the germinal
CC vesicle (GV) stage and stage 2 meiosis (MII) oocytes and pre-
CC implantation embryos until blastocyst stage (at protein level)
CC (PubMed:16571911, PubMed:23560077). {ECO:0000269|PubMed:16571911,
CC ECO:0000269|PubMed:23560077}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype (PubMed:28930672). Mice were
CC born at the expected Mendelian ratio and show no overt phenotype
CC (PubMed:28930672). Zygotes however display strongly reduced levels of
CC 5-hydroxymethylcytosine (5hmC) (PubMed:28930672).
CC {ECO:0000269|PubMed:28930672}.
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DR EMBL; AK005861; BAB24281.1; -; mRNA.
DR CCDS; CCDS50206.1; -.
DR RefSeq; NP_083574.1; NM_029298.1.
DR AlphaFoldDB; Q9DAG5; -.
DR STRING; 10090.ENSMUSP00000044250; -.
DR iPTMnet; Q9DAG5; -.
DR PhosphoSitePlus; Q9DAG5; -.
DR PaxDb; Q9DAG5; -.
DR PRIDE; Q9DAG5; -.
DR ProteomicsDB; 258647; -.
DR Antibodypedia; 62229; 37 antibodies from 7 providers.
DR Ensembl; ENSMUST00000040609; ENSMUSP00000044250; ENSMUSG00000036557.
DR GeneID; 75467; -.
DR KEGG; mmu:75467; -.
DR UCSC; uc008duw.2; mouse.
DR CTD; 285051; -.
DR MGI; MGI:1922717; Stpg4.
DR VEuPathDB; HostDB:ENSMUSG00000036557; -.
DR eggNOG; ENOG502S008; Eukaryota.
DR GeneTree; ENSGT00390000008095; -.
DR InParanoid; Q9DAG5; -.
DR OMA; SCFQSKV; -.
DR OrthoDB; 1292301at2759; -.
DR PhylomeDB; Q9DAG5; -.
DR TreeFam; TF329156; -.
DR BioGRID-ORCS; 75467; 0 hits in 71 CRISPR screens.
DR PRO; PR:Q9DAG5; -.
DR Proteomes; UP000000589; Chromosome 17.
DR RNAct; Q9DAG5; protein.
DR Bgee; ENSMUSG00000036557; Expressed in spermatocyte and 13 other tissues.
DR ExpressionAtlas; Q9DAG5; baseline and differential.
DR Genevisible; Q9DAG5; MM.
DR GO; GO:0005737; C:cytoplasm; IDA:MGI.
DR GO; GO:0001674; C:female germ cell nucleus; IDA:MGI.
DR GO; GO:0001939; C:female pronucleus; IDA:UniProtKB.
DR GO; GO:0042585; C:germinal vesicle; IDA:UniProtKB.
DR GO; GO:0001673; C:male germ cell nucleus; IDA:MGI.
DR GO; GO:0001940; C:male pronucleus; IDA:UniProtKB.
DR GO; GO:0003682; F:chromatin binding; IDA:UniProtKB.
DR GO; GO:0042393; F:histone binding; IDA:MGI.
DR GO; GO:0090116; P:C-5 methylation of cytosine; IMP:MGI.
DR GO; GO:0006325; P:chromatin organization; IEA:UniProtKB-KW.
DR GO; GO:0044727; P:DNA demethylation of male pronucleus; IMP:UniProtKB.
DR GO; GO:1901537; P:positive regulation of DNA demethylation; IMP:UniProtKB.
DR InterPro; IPR010736; SHIPPO-rpt.
DR InterPro; IPR039345; STPG4.
DR PANTHER; PTHR35678; PTHR35678; 1.
DR Pfam; PF07004; SHIPPO-rpt; 1.
PE 1: Evidence at protein level;
KW Chromatin regulator; Cytoplasm; Developmental protein; Nucleus;
KW Reference proteome.
FT CHAIN 1..247
FT /note="Protein STPG4"
FT /id="PRO_0000311685"
SQ SEQUENCE 247 AA; 27632 MW; 5A9BBD78EE7BD9FA CRC64;
MAEPTVVIAP TTVPREDLGH GEPLLMTAKQ VKKTPGLERE GWWRLTITDT PIPGTYHFRT
FTEEALLNPV KITYNFKNEG RKKLPLVLRN DPVPTDLPQY SPPDFLELLK KQTASYSFKD
KPRADPSTLV DKDESLHLCP GQYEVLPAPV PKSPARSFVF RSSVQRFPPN YFTPHEGPGP
GDYELKASPK GSITSCFRSK VPRFLPVSSK TPGPGAYTSS RQFPKQSSTI AKMGREHSLF
FNNTIGF
