ID   STP2_PONPY              Reviewed;         138 AA.
AC   Q9N1A4;
DT   27-SEP-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   03-AUG-2022, entry version 73.
DE   RecName: Full=Nuclear transition protein 2;
DE            Short=TP-2;
DE            Short=TP2;
DE   Flags: Fragment;
GN   Name=TNP2;
OS   Pongo pygmaeus (Bornean orangutan).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Pongo.
OX   NCBI_TaxID=9600;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=10659848; DOI=10.1038/35002070;
RA   Wyckoff G.J., Wang W., Wu C.-I.;
RT   "Rapid evolution of male reproductive genes in the descent of man.";
RL   Nature 403:304-309(2000).
CC   -!- FUNCTION: Plays a key role in the replacement of histones to protamine
CC       in the elongating spermatids of mammals. In condensing spermatids,
CC       loaded onto the nucleosomes, where it promotes the recruitment and
CC       processing of protamines, which are responsible for histone eviction.
CC       {ECO:0000250|UniProtKB:P11378}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:P11101}. Nucleus,
CC       nucleolus {ECO:0000250|UniProtKB:P11101}. Chromosome
CC       {ECO:0000250|UniProtKB:P11101}. Note=Loaded onto the nucleosomes of
CC       condensing spermatids (By similarity). Nuclear import is mediated by
CC       IPO4. Nucleolar localization requires the protein to be phosphorylated
CC       (By similarity). {ECO:0000250|UniProtKB:P11101,
CC       ECO:0000250|UniProtKB:P11378}.
CC   -!- TISSUE SPECIFICITY: Testis.
CC   -!- SIMILARITY: Belongs to the nuclear transition protein 2 family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AF215719; AAF35858.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q9N1A4; -.
DR   GO; GO:0000786; C:nucleosome; ISS:UniProtKB.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0010954; P:positive regulation of protein processing; ISS:UniProtKB.
DR   GO; GO:0035093; P:spermatogenesis, exchange of chromosomal proteins; ISS:UniProtKB.
DR   InterPro; IPR000678; TP2.
DR   PANTHER; PTHR17488; PTHR17488; 1.
DR   Pfam; PF01254; TP2; 1.
DR   PROSITE; PS00970; TP2_1; 1.
DR   PROSITE; PS00971; TP2_2; 1.
PE   2: Evidence at transcript level;
KW   Chromosome; Developmental protein; Differentiation; DNA-binding;
KW   Metal-binding; Nucleosome core; Nucleus; Phosphoprotein; Spermatogenesis;
KW   Zinc.
FT   CHAIN           1..>138
FT                   /note="Nuclear transition protein 2"
FT                   /id="PRO_0000191432"
FT   REGION          1..117
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           116..124
FT                   /note="Nuclear localization signal"
FT                   /evidence="ECO:0000250|UniProtKB:P11101"
FT   COMPBIAS        1..81
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         12
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000250|UniProtKB:P11101"
FT   BINDING         16
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000250|UniProtKB:P11101"
FT   BINDING         29
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000250|UniProtKB:P11101"
FT   BINDING         31
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000250|UniProtKB:P11101"
FT   MOD_RES         138
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:P11101"
FT   NON_TER         138
SQ   SEQUENCE   138 AA;  15585 MW;  FECB195793AB4D33 CRC64;
     MDTKTHSLPI THTQLHSNSQ PQSRTCSPCT CTRHRQTFSQ SCRQSQRGSW SQNSSQSPAS
     HHNPTGAHSS SGHQSQSPND SPPPKRHKKT MNSHHSPTRP TILHCSCPKN RKNFEGKLNK
     KKMAKRIQHV YKTKTRSS