SSNA1_CHLRE
ID SSNA1_CHLRE Reviewed; 111 AA.
AC Q9XF62;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1999, sequence version 1.
DT 03-AUG-2022, entry version 64.
DE RecName: Full=Microtubule nucleation factor SSNA1 {ECO:0000303|PubMed:30250060};
DE Short=CrSSNA1 {ECO:0000303|PubMed:30250060};
DE AltName: Full=13 kDa deflagellation-inducible protein {ECO:0000303|PubMed:12640030};
GN Name=SSNA1 {ECO:0000303|PubMed:30250060};
GN Synonyms=DIP13 {ECO:0000303|PubMed:12640030};
OS Chlamydomonas reinhardtii (Chlamydomonas smithii).
OC Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Chlorophyceae;
OC CS clade; Chlamydomonadales; Chlamydomonadaceae; Chlamydomonas.
OX NCBI_TaxID=3055;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION,
RP DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RC STRAIN=CC125+;
RX PubMed=12640030; DOI=10.1242/jcs.00337;
RA Pfannenschmid F., Wimmer V.C., Rios R.M., Geimer S., Kroeckel U.,
RA Leiherer A., Haller K., Nemcova Y., Mages W.;
RT "Chlamydomonas DIP13 and human NA14: a new class of proteins associated
RT with microtubule structures is involved in cell division.";
RL J. Cell Sci. 116:1449-1462(2003).
RN [2]
RP FUNCTION, SUBUNIT, AND MUTAGENESIS OF 20-GLU-ASP-21; GLU-20 AND
RP 105-LYS--LYS-107.
RX PubMed=30250060; DOI=10.1038/s41556-018-0199-8;
RA Basnet N., Nedozralova H., Crevenna A.H., Bodakuntla S., Schlichthaerle T.,
RA Taschner M., Cardone G., Janke C., Jungmann R., Magiera M.M.,
RA Biertuempfel C., Mizuno N.;
RT "Direct induction of microtubule branching by microtubule nucleation factor
RT SSNA1.";
RL Nat. Cell Biol. 20:1172-1180(2018).
CC -!- FUNCTION: Microtubule-binding protein which stabilizes dynamic
CC microtubules by slowing growth and shrinkage at both plus and minus
CC ends and serves as a sensor of microtubule damage (By similarity).
CC Induces microtubule branching which is mediated by the formation of
CC long SSNA1 fibrils which guide microtubule protofilaments to split
CC apart from the mother microtubule and form daughter microtubules
CC (PubMed:30250060). Required for cell division (PubMed:12640030).
CC {ECO:0000250|UniProtKB:O43805, ECO:0000269|PubMed:12640030,
CC ECO:0000269|PubMed:30250060}.
CC -!- SUBUNIT: Self-assembles into fibrils in a head-to-tail fashion.
CC {ECO:0000269|PubMed:30250060}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, flagellum basal body
CC {ECO:0000269|PubMed:12640030}. Cytoplasm, cytoskeleton, flagellum
CC axoneme {ECO:0000269|PubMed:12640030}.
CC -!- DEVELOPMENTAL STAGE: During cell division, levels are elevated between
CC hours 0 at the onset of division and 4 at mid-divison. After 5 hours,
CC when division is close to completion, levels decrease and stay low
CC until hour 7. {ECO:0000269|PubMed:12640030}.
CC -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown results in multinucleate,
CC multiflagellate cells. {ECO:0000269|PubMed:12640030}.
CC -!- SIMILARITY: Belongs to the SSNA1 family. {ECO:0000305}.
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DR EMBL; AF131736; AAD27849.1; -; Genomic_DNA.
DR RefSeq; XP_001697145.1; XM_001697093.1.
DR AlphaFoldDB; Q9XF62; -.
DR SMR; Q9XF62; -.
DR STRING; 3055.EDP00400; -.
DR PRIDE; Q9XF62; -.
DR ProMEX; Q9XF62; -.
DR EnsemblPlants; PNW70549; PNW70549; CHLRE_17g724550v5.
DR GeneID; 5722752; -.
DR Gramene; PNW70549; PNW70549; CHLRE_17g724550v5.
DR KEGG; cre:CHLRE_17g724550v5; -.
DR eggNOG; ENOG502S16M; Eukaryota.
DR HOGENOM; CLU_153440_0_0_1; -.
DR OMA; SYNNELV; -.
DR OrthoDB; 1513306at2759; -.
DR GO; GO:0005930; C:axoneme; IDA:UniProtKB.
DR GO; GO:0036064; C:ciliary basal body; IDA:UniProtKB.
DR GO; GO:0005881; C:cytoplasmic microtubule; IDA:UniProtKB.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0043621; F:protein self-association; IDA:UniProtKB.
DR GO; GO:0051301; P:cell division; IMP:UniProtKB.
DR GO; GO:0000226; P:microtubule cytoskeleton organization; IDA:UniProtKB.
DR InterPro; IPR033362; SSNA1_fam.
DR PANTHER; PTHR28661; PTHR28661; 1.
PE 1: Evidence at protein level;
KW Cell cycle; Cell division; Cell projection; Cilium; Coiled coil; Cytoplasm;
KW Cytoskeleton; Flagellum.
FT CHAIN 1..111
FT /note="Microtubule nucleation factor SSNA1"
FT /id="PRO_0000114485"
FT COILED 6..71
FT /evidence="ECO:0000255"
FT MUTAGEN 20..21
FT /note="ED->AA: Drastic reduction in microtubule branching.
FT Abolishes microtubule branching; when associated with 105-
FT A--A-107."
FT /evidence="ECO:0000269|PubMed:30250060"
FT MUTAGEN 20..21
FT /note="ED->KK: Abolishes microtubule branching; when
FT associated with 105-E--E-107."
FT /evidence="ECO:0000269|PubMed:30250060"
FT MUTAGEN 20
FT /note="E->A: Drastic reduction in microtubule branching."
FT /evidence="ECO:0000269|PubMed:30250060"
FT MUTAGEN 105..107
FT /note="KKK->AAA: Abolishes fibril formation and microtubule
FT branching. Abolishes microtubule branching; when associated
FT with 20-A-A-21."
FT /evidence="ECO:0000269|PubMed:30250060"
FT MUTAGEN 105..107
FT /note="KKK->EEE: Abolishes microtubule branching; when
FT associated with 20-K-K-21."
FT /evidence="ECO:0000269|PubMed:30250060"
SQ SEQUENCE 111 AA; 12711 MW; C9E4E87474AF1147 CRC64;
MSAQGQALQN HNNELVKCIE DLREKREEII KQLREDDAEK AKITQELQIL TKRLAQVNES
IARKTETKNE YDKVISETEA AYLKILESSQ TLLTVLKREA VNIAKKKQAS S
