SSL12_ARATH
ID SSL12_ARATH Reviewed; 335 AA.
AC P94111; Q9C9C3;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 06-JUN-2002, sequence version 2.
DT 03-AUG-2022, entry version 137.
DE RecName: Full=Protein STRICTOSIDINE SYNTHASE-LIKE 12 {ECO:0000303|PubMed:10777701};
DE Short=AtSSL12 {ECO:0000303|PubMed:10777701};
DE EC=4.3.3.2;
DE AltName: Full=Strictosidine synthase 1;
DE Short=SS-1;
DE AltName: Full=Strictosidine synthase 13 {ECO:0000303|PubMed:19121120};
DE Short=AtSS13 {ECO:0000303|PubMed:19121120};
DE Flags: Precursor;
GN Name=SSL12 {ECO:0000303|PubMed:10777701};
GN Synonyms=SS1, SS13 {ECO:0000303|PubMed:19121120};
GN OrderedLocusNames=At1g74020 {ECO:0000312|Araport:AT1G74020};
GN ORFNames=F2P9.11 {ECO:0000312|EMBL:AAG52513.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RC STRAIN=cv. Landsberg erecta;
RA Jain A.K., Nessler C.L.;
RL Submitted (DEC-1995) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=10777701; DOI=10.1006/bbrc.2000.2598;
RA Fabbri M., Delp G., Schmidt O., Theopold U.;
RT "Animal and plant members of a gene family with similarity to alkaloid-
RT synthesizing enzymes.";
RL Biochem. Biophys. Res. Commun. 271:191-196(2000).
RN [6]
RP INDUCTION BY POTASSIUM STARVATION.
RC STRAIN=cv. Wassilewskija-2;
RX PubMed=15489280; DOI=10.1104/pp.104.046672;
RA Hampton C.R., Bowen H.C., Broadley M.R., Hammond J.P., Mead A., Payne K.A.,
RA Pritchard J., White P.J.;
RT "Cesium toxicity in Arabidopsis.";
RL Plant Physiol. 136:3824-3837(2004).
RN [7]
RP GENE FAMILY.
RC STRAIN=cv. Columbia;
RX PubMed=19121120; DOI=10.1111/j.1438-8677.2008.00139.x;
RA Sohani M.M., Schenk P.M., Schultz C.J., Schmidt O.;
RT "Phylogenetic and transcriptional analysis of a strictosidine synthase-like
RT gene family in Arabidopsis thaliana reveals involvement in plant defence
RT responses.";
RL Plant Biol. 11:105-117(2009).
CC -!- FUNCTION: Catalyzes the stereospecific condensation of tryptamine with
CC secologanin to form strictosidine, the key intermediate of indole
CC alkaloid biosynthesis. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=3alpha(S)-strictosidine + H2O = secologanin + tryptamine;
CC Xref=Rhea:RHEA:15013, ChEBI:CHEBI:15377, ChEBI:CHEBI:18002,
CC ChEBI:CHEBI:57887, ChEBI:CHEBI:58193; EC=4.3.3.2;
CC -!- PATHWAY: Alkaloid biosynthesis; 3alpha(S)-strictosidine biosynthesis;
CC 3alpha(S)-strictosidine from secologanin and tryptamine: step 1/1.
CC -!- SUBCELLULAR LOCATION: Vacuole {ECO:0000250}.
CC -!- INDUCTION: Upon potassium (K) starvation.
CC {ECO:0000269|PubMed:15489280}.
CC -!- SIMILARITY: Belongs to the strictosidine synthase family.
CC {ECO:0000305}.
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DR EMBL; U43945; AAB40594.1; -; mRNA.
DR EMBL; U43713; AAB40593.1; -; Genomic_DNA.
DR EMBL; AC016662; AAG52513.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE35538.1; -; Genomic_DNA.
DR EMBL; AY034970; AAK59475.1; -; mRNA.
DR EMBL; AY062976; AAL34150.1; -; mRNA.
DR PIR; A96768; A96768.
DR RefSeq; NP_177542.1; NM_106061.4.
DR AlphaFoldDB; P94111; -.
DR SMR; P94111; -.
DR STRING; 3702.AT1G74020.1; -.
DR PaxDb; P94111; -.
DR PRIDE; P94111; -.
DR ProteomicsDB; 228377; -.
DR EnsemblPlants; AT1G74020.1; AT1G74020.1; AT1G74020.
DR GeneID; 843740; -.
DR Gramene; AT1G74020.1; AT1G74020.1; AT1G74020.
DR KEGG; ath:AT1G74020; -.
DR Araport; AT1G74020; -.
DR TAIR; locus:2031486; AT1G74020.
DR eggNOG; KOG1520; Eukaryota.
DR HOGENOM; CLU_023267_2_1_1; -.
DR OMA; RYTPIQV; -.
DR OrthoDB; 757814at2759; -.
DR PhylomeDB; P94111; -.
DR BioCyc; ARA:AT1G74020-MON; -.
DR UniPathway; UPA00311; UER00447.
DR PRO; PR:P94111; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; P94111; baseline and differential.
DR Genevisible; P94111; AT.
DR GO; GO:0005794; C:Golgi apparatus; HDA:TAIR.
DR GO; GO:0005739; C:mitochondrion; HDA:TAIR.
DR GO; GO:0000325; C:plant-type vacuole; HDA:TAIR.
DR GO; GO:0005886; C:plasma membrane; HDA:TAIR.
DR GO; GO:0009506; C:plasmodesma; HDA:TAIR.
DR GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
DR GO; GO:0009820; P:alkaloid metabolic process; IEA:UniProtKB-KW.
DR GO; GO:0009058; P:biosynthetic process; IEA:InterPro.
DR GO; GO:0051365; P:cellular response to potassium ion starvation; IEP:UniProtKB.
DR GO; GO:0009753; P:response to jasmonic acid; IEP:TAIR.
DR GO; GO:0009611; P:response to wounding; IEP:TAIR.
DR Gene3D; 2.120.10.30; -; 1.
DR InterPro; IPR011042; 6-blade_b-propeller_TolB-like.
DR InterPro; IPR018119; Strictosidine_synth_cons-reg.
DR Pfam; PF03088; Str_synth; 1.
PE 2: Evidence at transcript level;
KW Alkaloid metabolism; Glycoprotein; Lyase; Reference proteome; Signal;
KW Vacuole.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT CHAIN 23..335
FT /note="Protein STRICTOSIDINE SYNTHASE-LIKE 12"
FT /id="PRO_0000033330"
FT CARBOHYD 80
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CONFLICT 19
FT /note="A -> P (in Ref. 1; AAB40594/AAB40593)"
FT /evidence="ECO:0000305"
FT CONFLICT 327
FT /note="L -> I (in Ref. 1; AAB40594/AAB40593)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 335 AA; 35293 MW; C1382BF040A72849 CRC64;
MTSFCSMISL LLLLSLSSAV FSDDASFQKL PVPETRSGPE AFAFDSTGKG FYTGVSGGKI
LKYLPETGYV DFAQITESSN SSWCDGTIGT ALAGRCGRPA GIAFNEKTGD LYVADAPLGL
HVISPAGGLA TKITDSVDGK PFKFLDGLDV DPTTGVVYFT SFSSRFSPIQ VLIALGLKDA
TGKLYKYDPS TKVVTVLMEG LSGSAGCAVS SDGSFVLVSQ FTKSNIKRYW IKGPKAGSSE
DFTNSVSNPD NIKRIGSTGN FWVASVVNKI IVPTNPSAVK VNSNGEVLQT IPLKDKFGDT
LLSEVNEFEG NLYIGTLTGP FAGILKLEKG SCPAT
