SPAS2_MOUSE
ID SPAS2_MOUSE Reviewed; 545 AA.
AC Q8K1N4; Q3UX77; Q8C810; Q8R1P6;
DT 23-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 03-AUG-2022, entry version 116.
DE RecName: Full=Spermatogenesis-associated serine-rich protein 2;
DE AltName: Full=Serine-rich spermatocytes and round spermatid 59 kDa protein;
DE AltName: Full=p59scr;
GN Name=Spats2; Synonyms=Scr59;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), SUBCELLULAR LOCATION, DEVELOPMENTAL
RP STAGE, AND TISSUE SPECIFICITY.
RC TISSUE=Testis;
RX PubMed=11944913; DOI=10.1006/bbrc.2002.6769;
RA Senoo M., Hoshino S., Mochida N., Matsumura Y., Habu S.;
RT "Identification of a novel protein p59(scr), which is expressed at specific
RT stages of mouse spermatogenesis.";
RL Biochem. Biophys. Res. Commun. 292:992-998(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=C57BL/6J; TISSUE=Cerebellum, and Egg;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 13-545 (ISOFORM 1).
RC STRAIN=FVB/N; TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=17242355; DOI=10.1073/pnas.0609836104;
RA Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.;
RT "Large-scale phosphorylation analysis of mouse liver.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-142; SER-145 AND SER-147, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Lung, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:11944913}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8K1N4-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8K1N4-2; Sequence=VSP_028790, VSP_028791;
CC -!- TISSUE SPECIFICITY: Detected in testis, in spermatocytes and round
CC spermatids (at protein level). Highly expressed in testis, and detected
CC at lower levels in brain, heart, thymus, skeletal muscle, ovary,
CC stomach and lung. {ECO:0000269|PubMed:11944913}.
CC -!- DEVELOPMENTAL STAGE: Detected in newborns. Levels increase during the
CC first four weeks, and then remain at the same stable level.
CC {ECO:0000269|PubMed:11944913}.
CC -!- SIMILARITY: Belongs to the SPATS2 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH23432.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AB079132; BAB91237.1; -; mRNA.
DR EMBL; AK048720; BAC33432.1; -; mRNA.
DR EMBL; AK135838; BAE22686.1; -; mRNA.
DR EMBL; BC023432; AAH23432.1; ALT_INIT; mRNA.
DR CCDS; CCDS27816.1; -. [Q8K1N4-1]
DR RefSeq; NP_631879.1; NM_139140.1. [Q8K1N4-1]
DR RefSeq; XP_011244039.1; XM_011245737.1. [Q8K1N4-1]
DR AlphaFoldDB; Q8K1N4; -.
DR SMR; Q8K1N4; -.
DR BioGRID; 215446; 9.
DR IntAct; Q8K1N4; 2.
DR MINT; Q8K1N4; -.
DR STRING; 10090.ENSMUSP00000070549; -.
DR iPTMnet; Q8K1N4; -.
DR PhosphoSitePlus; Q8K1N4; -.
DR EPD; Q8K1N4; -.
DR MaxQB; Q8K1N4; -.
DR PaxDb; Q8K1N4; -.
DR PeptideAtlas; Q8K1N4; -.
DR PRIDE; Q8K1N4; -.
DR ProteomicsDB; 261566; -. [Q8K1N4-1]
DR ProteomicsDB; 261567; -. [Q8K1N4-2]
DR Antibodypedia; 48974; 49 antibodies from 12 providers.
DR DNASU; 72572; -.
DR Ensembl; ENSMUST00000063517; ENSMUSP00000070549; ENSMUSG00000051934. [Q8K1N4-1]
DR GeneID; 72572; -.
DR KEGG; mmu:72572; -.
DR UCSC; uc007xov.1; mouse. [Q8K1N4-1]
DR UCSC; uc007xow.1; mouse. [Q8K1N4-2]
DR CTD; 65244; -.
DR MGI; MGI:1919822; Spats2.
DR VEuPathDB; HostDB:ENSMUSG00000051934; -.
DR eggNOG; ENOG502QY9Y; Eukaryota.
DR GeneTree; ENSGT00390000001138; -.
DR HOGENOM; CLU_037089_0_0_1; -.
DR InParanoid; Q8K1N4; -.
DR OMA; SEWGQAH; -.
DR OrthoDB; 1523681at2759; -.
DR PhylomeDB; Q8K1N4; -.
DR TreeFam; TF320553; -.
DR BioGRID-ORCS; 72572; 3 hits in 74 CRISPR screens.
DR ChiTaRS; Spats2; mouse.
DR PRO; PR:Q8K1N4; -.
DR Proteomes; UP000000589; Chromosome 15.
DR RNAct; Q8K1N4; protein.
DR Bgee; ENSMUSG00000051934; Expressed in primary oocyte and 253 other tissues.
DR ExpressionAtlas; Q8K1N4; baseline and differential.
DR Genevisible; Q8K1N4; MM.
DR GO; GO:0005737; C:cytoplasm; IDA:MGI.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR InterPro; IPR009816; DUF1387.
DR InterPro; IPR009060; UBA-like_sf.
DR PANTHER; PTHR15623; PTHR15623; 1.
DR Pfam; PF07139; DUF1387; 1.
DR SUPFAM; SSF46934; SSF46934; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytoplasm; Phosphoprotein; Reference proteome.
FT CHAIN 1..545
FT /note="Spermatogenesis-associated serine-rich protein 2"
FT /id="PRO_0000307698"
FT REGION 87..133
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 202..232
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 390..545
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 103..129
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 395..410
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 452..487
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 504..518
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 142
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 145
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 147
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 520
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q86XZ4"
FT VAR_SEQ 337..361
FT /note="HFVSERKYDEDLGRVARFTCDVETL -> VKLPSSFIDSLDQPAQQISRSHG
FT FT (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_028790"
FT VAR_SEQ 362..545
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_028791"
SQ SEQUENCE 545 AA; 58956 MW; FABF2DE0542EE174 CRC64;
MSRKQSQKDS SGFIFDLQSN TVLAQGGTFE NMKEKINAVR AIVPNKSNNE IILVLQHFDN
CVDKTVQAFM EGSASEVLKE WIVTGKKKNK KKKSKPKPAS EASGSAPDSS KSAPIQEEQP
ASSEKGSING YHVNGAINDA ESVDSLSEGL ETLSIDAREL EDPEFAAAET LDRTGSVLEN
GVSDFEPKSL TAHSISNVQQ SRNAAKSLSR TTPGAQVSNL GMENVPLSST NKKLGSNIEK
SVKDLQRCTV SLARYRVVVK EEMDASIKKM KQAFAELQSC LMDREVALLA EMDKVKAEAM
EILLSRQKKA ELLKKMTDVA VRMSEEQLVE LRADIKHFVS ERKYDEDLGR VARFTCDVET
LKQSIDSFGQ VSHPKNSYST RSRCSLVAPV SLSGPSDGSA ASSSPDASVP SLPGANKRNC
APREASAAMT NSSDRPCQAH REVFPGNRRG GQGYRAQSQK TADPSNPGRH DSVGRYRNSS
WYSSGPRYQG VPPQAPGNAG ERSRPYSAGT NGTGAISEPS PPKPSFKKGL PQRKPRASQA
EAANS
