SPAS1_MOUSE
ID SPAS1_MOUSE Reviewed; 269 AA.
AC A2RRY8; Q6VNB9; Q9CUD9; Q9DAG2;
DT 23-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT 06-MAR-2007, sequence version 1.
DT 03-AUG-2022, entry version 83.
DE RecName: Full=Spermatogenesis-associated serine-rich protein 1;
DE AltName: Full=Dvl2-DEP domain-interacting protein;
GN Name=Spats1; Synonyms=Ddip;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
RC STRAIN=BALB/cJ; TISSUE=Embryo;
RA Ng S.S., Han L., Zhang X., He X., Chang Z.;
RT "A novel protein interacting with Dvl2.";
RL Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
RC STRAIN=C57BL/6J; TISSUE=Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-54, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=A2RRY8-1; Sequence=Displayed;
CC Name=2;
CC IsoId=A2RRY8-2; Sequence=VSP_028789;
CC Name=3;
CC IsoId=A2RRY8-3; Sequence=VSP_028788, VSP_028789;
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DR EMBL; AY336501; AAQ88273.1; -; mRNA.
DR EMBL; AK005865; BAB24285.1; -; mRNA.
DR EMBL; AK016586; BAB30325.1; -; mRNA.
DR EMBL; BC131909; AAI31910.1; -; mRNA.
DR CCDS; CCDS50118.1; -. [A2RRY8-1]
DR CCDS; CCDS89102.1; -. [A2RRY8-3]
DR CCDS; CCDS89103.1; -. [A2RRY8-2]
DR RefSeq; NP_081925.2; NM_027649.3. [A2RRY8-1]
DR RefSeq; XP_006524978.1; XM_006524915.2.
DR RefSeq; XP_006524980.1; XM_006524917.2.
DR AlphaFoldDB; A2RRY8; -.
DR BioGRID; 214418; 6.
DR STRING; 10090.ENSMUSP00000024731; -.
DR iPTMnet; A2RRY8; -.
DR PhosphoSitePlus; A2RRY8; -.
DR MaxQB; A2RRY8; -.
DR PaxDb; A2RRY8; -.
DR PRIDE; A2RRY8; -.
DR ProteomicsDB; 261563; -. [A2RRY8-1]
DR ProteomicsDB; 261564; -. [A2RRY8-2]
DR ProteomicsDB; 261565; -. [A2RRY8-3]
DR Antibodypedia; 56365; 115 antibodies from 20 providers.
DR Ensembl; ENSMUST00000024731; ENSMUSP00000024731; ENSMUSG00000023935. [A2RRY8-1]
DR Ensembl; ENSMUST00000233553; ENSMUSP00000156716; ENSMUSG00000023935. [A2RRY8-2]
DR Ensembl; ENSMUST00000233769; ENSMUSP00000156469; ENSMUSG00000023935. [A2RRY8-3]
DR GeneID; 71020; -.
DR KEGG; mmu:71020; -.
DR UCSC; uc008cqn.1; mouse. [A2RRY8-2]
DR UCSC; uc008cqo.1; mouse. [A2RRY8-3]
DR UCSC; uc008cqp.1; mouse. [A2RRY8-1]
DR CTD; 221409; -.
DR MGI; MGI:1918270; Spats1.
DR VEuPathDB; HostDB:ENSMUSG00000023935; -.
DR eggNOG; ENOG502S39M; Eukaryota.
DR GeneTree; ENSGT00390000006786; -.
DR HOGENOM; CLU_081878_0_0_1; -.
DR InParanoid; A2RRY8; -.
DR OMA; IPTLPFW; -.
DR OrthoDB; 1532791at2759; -.
DR PhylomeDB; A2RRY8; -.
DR TreeFam; TF337302; -.
DR BioGRID-ORCS; 71020; 1 hit in 71 CRISPR screens.
DR ChiTaRS; Spats1; mouse.
DR PRO; PR:A2RRY8; -.
DR Proteomes; UP000000589; Chromosome 17.
DR RNAct; A2RRY8; protein.
DR Bgee; ENSMUSG00000023935; Expressed in spermatocyte and 58 other tissues.
DR Genevisible; A2RRY8; MM.
DR InterPro; IPR029165; SASRP1.
DR PANTHER; PTHR35845; PTHR35845; 1.
DR Pfam; PF15160; SASRP1; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Phosphoprotein; Reference proteome.
FT CHAIN 1..269
FT /note="Spermatogenesis-associated serine-rich protein 1"
FT /id="PRO_0000307695"
FT REGION 1..92
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 17..42
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 59..86
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 54
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 72
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q811V6"
FT MOD_RES 75
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q811V6"
FT MOD_RES 82
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q811V6"
FT VAR_SEQ 202..222
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_028788"
FT VAR_SEQ 262..269
FT /note="ASNFSRQQ -> TGEMAPRLKVLAALPEGTSSVLSTQGRLTTPCSSSIRGSQ
FT TLFLPHRTSSHVLRTYTRTLHINKKP (in isoform 2 and isoform 3)"
FT /evidence="ECO:0000303|PubMed:16141072, ECO:0000303|Ref.1"
FT /id="VSP_028789"
FT CONFLICT 61..62
FT /note="EL -> DV (in Ref. 2; BAB24285)"
FT /evidence="ECO:0000305"
FT CONFLICT 90
FT /note="I -> D (in Ref. 2; BAB24285)"
FT /evidence="ECO:0000305"
FT CONFLICT 208
FT /note="F -> L (in Ref. 2; BAB24285)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 269 AA; 30169 MW; DD895E4897EE737F CRC64;
MESSKDTQHG DALESKSCLA NRTSSRQNKR TSLSSSDGTG PRVTESLGLP RVLTPSDTAA
ELGQKTSSSS SSSSSSAQSN RSSKVSLPEI PKEKYPEEFS LLNSQTEDGQ RPEWTFYPRF
SSNIHTYHIG KQCFFNGVFR GNRRSVAERT VDNSLGKKKY DIDPRNGIPK LTPGDNPYMF
PEQSKEFFKA GATLPPVNFS LGPYEKKFDT FIPLEPLPKI PNLPFWEKEK ANNLKNEIKE
VEELDNWQVP MPFLHGFFST GASNFSRQQ
