ID   SPAR_MOUSE              Reviewed;          75 AA.
AC   A0A1B0GSZ0;
DT   15-FEB-2017, integrated into UniProtKB/Swiss-Prot.
DT   05-OCT-2016, sequence version 1.
DT   03-AUG-2022, entry version 27.
DE   RecName: Full=Small regulatory polypeptide of amino acid response {ECO:0000303|PubMed:28024296};
GN   Name=Spaar {ECO:0000250|UniProtKB:A0A1B0GVQ0};
GN   Synonyms=Spar {ECO:0000303|PubMed:28024296};
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C57BL/6J;
RX   PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA   Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA   Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA   Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA   Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA   Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA   Eichler E.E., Ponting C.P.;
RT   "Lineage-specific biology revealed by a finished genome assembly of the
RT   mouse.";
RL   PLoS Biol. 7:E1000112-E1000112(2009).
RN   [2]
RP   FUNCTION, TISSUE SPECIFICITY, INDUCTION, DISRUPTION PHENOTYPE, AND
RP   INTERACTION WITH ATP6V0A1.
RX   PubMed=28024296; DOI=10.1038/nature21034;
RA   Matsumoto A., Pasut A., Matsumoto M., Yamashita R., Fung J., Monteleone E.,
RA   Saghatelian A., Nakayama K.I., Clohessy J.G., Pandolfi P.P.;
RT   "mTORC1 and muscle regeneration are regulated by the LINC00961-encoded SPAR
RT   polypeptide.";
RL   Nature 541:228-232(2017).
CC   -!- FUNCTION: Negative regulator of amino acid sensing and mTORC1, a
CC       signaling complex promoting cell growth in response to growth factors,
CC       energy levels and amino acids (By similarity). Negatively regulates
CC       mTORC1 activation by inhibiting recruitment of mTORC1 to lysosomes upon
CC       stimulation with amino acids: acts by promoting the formation of a
CC       tightly bound supercomplex composed of the lysosomal V-ATPase,
CC       Ragulator and Rag GTPases, preventing recruitment of mTORC1 (By
CC       similarity). Acts as a regulator of muscle regeneration following
CC       injury by regulating mTORC1 activation (PubMed:28024296).
CC       {ECO:0000250|UniProtKB:A0A1B0GVQ0, ECO:0000269|PubMed:28024296}.
CC   -!- SUBUNIT: Interacts with components of the lysosomal V-ATPase complex
CC       (By similarity). Interacts with ATP6V0A1 (PubMed:28024296). Interacts
CC       with ATP6V0A2 (By similarity). {ECO:0000250|UniProtKB:A0A1B0GVQ0}.
CC   -!- SUBCELLULAR LOCATION: Late endosome membrane
CC       {ECO:0000250|UniProtKB:A0A1B0GVQ0}; Single-pass membrane protein
CC       {ECO:0000250|UniProtKB:A0A1B0GVQ0}. Lysosome membrane
CC       {ECO:0000250|UniProtKB:A0A1B0GVQ0}; Single-pass membrane protein
CC       {ECO:0000250|UniProtKB:A0A1B0GVQ0}.
CC   -!- TISSUE SPECIFICITY: Expressed in the skeletal muscle.
CC       {ECO:0000269|PubMed:28024296}.
CC   -!- INDUCTION: Down-regulated in skeletal muscle upon acute injury.
CC       {ECO:0000269|PubMed:28024296}.
CC   -!- DISRUPTION PHENOTYPE: No visible phenotype. Mice were born at expected
CC       Mendelian ratio with no gross abnormalities.
CC       {ECO:0000269|PubMed:28024296}.
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DR   EMBL; AL773539; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AL824712; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   CCDS; CCDS89739.1; -.
DR   RefSeq; NP_001335037.1; NM_001348108.1.
DR   AlphaFoldDB; A0A1B0GSZ0; -.
DR   SMR; A0A1B0GSZ0; -.
DR   Antibodypedia; 79062; 2 antibodies from 2 providers.
DR   DNASU; 71406; -.
DR   Ensembl; ENSMUST00000131248; ENSMUSP00000148128; ENSMUSG00000028475.
DR   GeneID; 71406; -.
DR   KEGG; mmu:71406; -.
DR   CTD; 158376; -.
DR   MGI; MGI:1918656; Spaar.
DR   VEuPathDB; HostDB:ENSMUSG00000028475; -.
DR   GeneTree; ENSGT00850000133592; -.
DR   OMA; ILCCFSC; -.
DR   ChiTaRS; Sipa1l1; mouse.
DR   PRO; PR:A0A1B0GSZ0; -.
DR   Proteomes; UP000000589; Chromosome 4.
DR   RNAct; A0A1B0GSZ0; protein.
DR   Bgee; ENSMUSG00000028475; Expressed in gastrula and 57 other tissues.
DR   GO; GO:1905103; C:integral component of lysosomal membrane; ISS:UniProtKB.
DR   GO; GO:0031902; C:late endosome membrane; ISS:UniProtKB.
DR   GO; GO:0046611; C:lysosomal proton-transporting V-type ATPase complex; ISO:MGI.
DR   GO; GO:0071230; P:cellular response to amino acid stimulus; IMP:UniProtKB.
DR   GO; GO:1904262; P:negative regulation of TORC1 signaling; IMP:UniProtKB.
DR   GO; GO:0043416; P:regulation of skeletal muscle tissue regeneration; IMP:UniProtKB.
PE   1: Evidence at protein level;
KW   Endosome; Lysosome; Membrane; Reference proteome; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..75
FT                   /note="Small regulatory polypeptide of amino acid response"
FT                   /id="PRO_0000439039"
FT   TOPO_DOM        1..3
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000250|UniProtKB:A0A1B0GVQ0"
FT   TRANSMEM        4..24
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        25..75
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000250|UniProtKB:A0A1B0GVQ0"
SQ   SEQUENCE   75 AA;  8470 MW;  C1B0D2A8C9FC8AAC CRC64;
     METAVIGMVA VLFVITMAIT CILCYFSYDS HTQDPERSSR RSFTVATFHQ EASLFTGPAL
     QSRPLPRPQN FWTVV