SPAC7_HUMAN
ID SPAC7_HUMAN Reviewed; 195 AA.
AC Q96KW9; Q5T8L1;
DT 06-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT 06-FEB-2007, sequence version 2.
DT 03-AUG-2022, entry version 124.
DE RecName: Full=Sperm acrosome-associated protein 7 {ECO:0000312|HGNC:HGNC:29575};
DE Flags: Precursor;
GN Name=SPACA7 {ECO:0000312|HGNC:HGNC:29575}; Synonyms=C13orf28;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15057823; DOI=10.1038/nature02379;
RA Dunham A., Matthews L.H., Burton J., Ashurst J.L., Howe K.L.,
RA Ashcroft K.J., Beare D.M., Burford D.C., Hunt S.E., Griffiths-Jones S.,
RA Jones M.C., Keenan S.J., Oliver K., Scott C.E., Ainscough R., Almeida J.P.,
RA Ambrose K.D., Andrews D.T., Ashwell R.I.S., Babbage A.K., Bagguley C.L.,
RA Bailey J., Bannerjee R., Barlow K.F., Bates K., Beasley H., Bird C.P.,
RA Bray-Allen S., Brown A.J., Brown J.Y., Burrill W., Carder C., Carter N.P.,
RA Chapman J.C., Clamp M.E., Clark S.Y., Clarke G., Clee C.M., Clegg S.C.,
RA Cobley V., Collins J.E., Corby N., Coville G.J., Deloukas P., Dhami P.,
RA Dunham I., Dunn M., Earthrowl M.E., Ellington A.G., Faulkner L.,
RA Frankish A.G., Frankland J., French L., Garner P., Garnett J.,
RA Gilbert J.G.R., Gilson C.J., Ghori J., Grafham D.V., Gribble S.M.,
RA Griffiths C., Hall R.E., Hammond S., Harley J.L., Hart E.A., Heath P.D.,
RA Howden P.J., Huckle E.J., Hunt P.J., Hunt A.R., Johnson C., Johnson D.,
RA Kay M., Kimberley A.M., King A., Laird G.K., Langford C.J., Lawlor S.,
RA Leongamornlert D.A., Lloyd D.M., Lloyd C., Loveland J.E., Lovell J.,
RA Martin S., Mashreghi-Mohammadi M., McLaren S.J., McMurray A., Milne S.,
RA Moore M.J.F., Nickerson T., Palmer S.A., Pearce A.V., Peck A.I., Pelan S.,
RA Phillimore B., Porter K.M., Rice C.M., Searle S., Sehra H.K., Shownkeen R.,
RA Skuce C.D., Smith M., Steward C.A., Sycamore N., Tester J., Thomas D.W.,
RA Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P.,
RA Whitehead S.L., Willey D.L., Wilming L., Wray P.W., Wright M.W., Young L.,
RA Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Beck S., Bentley D.R.,
RA Rogers J., Ross M.T.;
RT "The DNA sequence and analysis of human chromosome 13.";
RL Nature 428:522-528(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANT ASP-111.
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=22495889; DOI=10.1530/rep-11-0270;
RA Korfanty J., Toma A., Wojtas A., Rusin A., Vydra N., Widlak W.;
RT "Identification of a new mouse sperm acrosome-associated protein.";
RL Reproduction 143:749-757(2012).
RN [4]
RP VARIANT [LARGE SCALE ANALYSIS] TRP-25.
RX PubMed=16959974; DOI=10.1126/science.1133427;
RA Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D.,
RA Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P.,
RA Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V.,
RA Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H.,
RA Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W.,
RA Velculescu V.E.;
RT "The consensus coding sequences of human breast and colorectal cancers.";
RL Science 314:268-274(2006).
CC -!- FUNCTION: Involved in fertilization. Seems not to play a direct role in
CC sperm-egg binding or gamete fusion. {ECO:0000250|UniProtKB:Q9D2S4}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:Q9D2S4}.
CC Cytoplasmic vesicle, secretory vesicle, acrosome
CC {ECO:0000269|PubMed:22495889}. Cytoplasmic vesicle, secretory vesicle,
CC acrosome lumen {ECO:0000250|UniProtKB:Q9D2S4}. Note=Localized in
CC perinuclear pro-acrosomal granules in round spermatides. Localized
CC between the inner and outer acrosomal membranes (matrix or lumen) in
CC spermatozoa. Secreted during acrosome exocytosis.
CC {ECO:0000250|UniProtKB:Q9D2S4}.
CC -!- TISSUE SPECIFICITY: Expressed in spermatozoa (at protein level)
CC (PubMed:22495889). {ECO:0000269|PubMed:22495889}.
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DR EMBL; AL160033; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC016750; AAH16750.1; -; mRNA.
DR CCDS; CCDS9524.1; -.
DR RefSeq; NP_660291.2; NM_145248.4.
DR AlphaFoldDB; Q96KW9; -.
DR BioGRID; 125763; 2.
DR IntAct; Q96KW9; 1.
DR STRING; 9606.ENSP00000283550; -.
DR GlyGen; Q96KW9; 2 sites.
DR iPTMnet; Q96KW9; -.
DR PhosphoSitePlus; Q96KW9; -.
DR BioMuta; SPACA7; -.
DR DMDM; 125863833; -.
DR MassIVE; Q96KW9; -.
DR PaxDb; Q96KW9; -.
DR PeptideAtlas; Q96KW9; -.
DR PRIDE; Q96KW9; -.
DR ProteomicsDB; 77126; -.
DR TopDownProteomics; Q96KW9; -.
DR Antibodypedia; 42580; 61 antibodies from 13 providers.
DR DNASU; 122258; -.
DR Ensembl; ENST00000283550.8; ENSP00000283550.3; ENSG00000153498.12.
DR Ensembl; ENST00000610393.2; ENSP00000480272.1; ENSG00000275865.2.
DR GeneID; 122258; -.
DR KEGG; hsa:122258; -.
DR MANE-Select; ENST00000283550.8; ENSP00000283550.3; NM_145248.5; NP_660291.2.
DR UCSC; uc001vsd.3; human.
DR CTD; 122258; -.
DR DisGeNET; 122258; -.
DR GeneCards; SPACA7; -.
DR HGNC; HGNC:29575; SPACA7.
DR HPA; ENSG00000153498; Tissue enriched (testis).
DR neXtProt; NX_Q96KW9; -.
DR OpenTargets; ENSG00000153498; -.
DR PharmGKB; PA147358486; -.
DR VEuPathDB; HostDB:ENSG00000153498; -.
DR eggNOG; ENOG502TF3C; Eukaryota.
DR GeneTree; ENSGT00390000018090; -.
DR HOGENOM; CLU_120537_0_0_1; -.
DR InParanoid; Q96KW9; -.
DR OMA; MAVNQGE; -.
DR OrthoDB; 1489307at2759; -.
DR PhylomeDB; Q96KW9; -.
DR TreeFam; TF338340; -.
DR PathwayCommons; Q96KW9; -.
DR SignaLink; Q96KW9; -.
DR BioGRID-ORCS; 122258; 9 hits in 1059 CRISPR screens.
DR GenomeRNAi; 122258; -.
DR Pharos; Q96KW9; Tdark.
DR PRO; PR:Q96KW9; -.
DR Proteomes; UP000005640; Chromosome 13.
DR RNAct; Q96KW9; protein.
DR Bgee; ENSG00000153498; Expressed in left testis and 52 other tissues.
DR ExpressionAtlas; Q96KW9; baseline and differential.
DR Genevisible; Q96KW9; HS.
DR GO; GO:0043160; C:acrosomal lumen; IEA:UniProtKB-SubCell.
DR GO; GO:0001669; C:acrosomal vesicle; IDA:MGI.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0007338; P:single fertilization; IEA:UniProtKB-KW.
DR InterPro; IPR029301; SPACA7.
DR Pfam; PF15307; SPACA7; 1.
PE 1: Evidence at protein level;
KW Cytoplasmic vesicle; Fertilization; Glycoprotein; Reference proteome;
KW Secreted; Signal.
FT SIGNAL 1..24
FT /evidence="ECO:0000255"
FT CHAIN 25..195
FT /note="Sperm acrosome-associated protein 7"
FT /id="PRO_0000274330"
FT REGION 64..89
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 119..158
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 64..82
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 134..158
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 62
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 159
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VARIANT 25
FT /note="R -> W (in a colorectal cancer sample; somatic
FT mutation; dbSNP:rs376712879)"
FT /evidence="ECO:0000269|PubMed:16959974"
FT /id="VAR_035675"
FT VARIANT 111
FT /note="V -> D (in dbSNP:rs10816)"
FT /evidence="ECO:0000269|PubMed:15489334"
FT /id="VAR_030260"
SQ SEQUENCE 195 AA; 21466 MW; 09E285617F560FD8 CRC64;
MAVSQGDGTL CFVLLLCCWQ ETELRPRTVI PGSPTEIPFS SKQEDMSELL DEILVQEILD
LNKTTPSEMP STASTLSTPL HAGIDENYQA GGSENYHELL ENLQFSPGIE VKISNDEANA
NANLHGDPSE NYRGPQVSPG SEKSVSSKEK NSKNTQYENL SILDQILQNI GRSSGNIFHK
EQQRTSAQRR SQGSQ
