ID   SP2SA_BACCR             Reviewed;         245 AA.
AC   Q81DD1;
DT   22-FEB-2012, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   25-MAY-2022, entry version 68.
DE   RecName: Full=Stage II sporulation protein SA;
DE   AltName: Full=Killer protein SpoIISA;
DE   AltName: Full=Toxin SpoIISA;
GN   Name=spoIISA; OrderedLocusNames=BC_2436;
OS   Bacillus cereus (strain ATCC 14579 / DSM 31 / CCUG 7414 / JCM 2152 / NBRC
OS   15305 / NCIMB 9373 / NCTC 2599 / NRRL B-3711).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC   Bacillus cereus group.
OX   NCBI_TaxID=226900;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 14579 / DSM 31 / CCUG 7414 / JCM 2152 / NBRC 15305 / NCIMB 9373
RC   / NCTC 2599 / NRRL B-3711;
RX   PubMed=12721630; DOI=10.1038/nature01582;
RA   Ivanova N., Sorokin A., Anderson I., Galleron N., Candelon B., Kapatral V.,
RA   Bhattacharyya A., Reznik G., Mikhailova N., Lapidus A., Chu L., Mazur M.,
RA   Goltsman E., Larsen N., D'Souza M., Walunas T., Grechkin Y., Pusch G.,
RA   Haselkorn R., Fonstein M., Ehrlich S.D., Overbeek R., Kyrpides N.C.;
RT   "Genome sequence of Bacillus cereus and comparative analysis with Bacillus
RT   anthracis.";
RL   Nature 423:87-91(2003).
RN   [2]
RP   FUNCTION AS A TOXIN, AND EXPRESSION IN E.COLI.
RC   STRAIN=ATCC 14579 / DSM 31 / CCUG 7414 / JCM 2152 / NBRC 15305 / NCIMB 9373
RC   / NCTC 2599 / NRRL B-3711;
RX   PubMed=18096016; DOI=10.1111/j.1574-6968.2007.00984.x;
RA   Florek P., Muchova K., Pavelcikova P., Barak I.;
RT   "Expression of functional Bacillus SpoIISAB toxin-antitoxin modules in
RT   Escherichia coli.";
RL   FEMS Microbiol. Lett. 278:177-184(2008).
CC   -!- FUNCTION: Toxic component of a type II toxin-antitoxin (TA) system.
CC       Upon expression in E.coli growth ceases. Upon coexpression with SpoIISB
CC       in E.coli normal growth is restored. {ECO:0000269|PubMed:18096016}.
CC   -!- SUBUNIT: Probably forms a complex with SpoIISB in which SpoIISB
CC       neutralizes the toxic activity.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the SpoIISA toxin family. {ECO:0000305}.
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DR   EMBL; AE016877; AAP09399.1; -; Genomic_DNA.
DR   RefSeq; NP_832198.1; NC_004722.1.
DR   RefSeq; WP_000624977.1; NZ_CP034551.1.
DR   AlphaFoldDB; Q81DD1; -.
DR   SMR; Q81DD1; -.
DR   EnsemblBacteria; AAP09399; AAP09399; BC_2436.
DR   GeneID; 67507119; -.
DR   KEGG; bce:BC2436; -.
DR   PATRIC; fig|226900.8.peg.2465; -.
DR   HOGENOM; CLU_1127307_0_0_9; -.
DR   OMA; IIVPFEH; -.
DR   Proteomes; UP000001417; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR   InterPro; IPR025940; SpoIISA_toxin.
DR   Pfam; PF14171; SpoIISA_toxin; 1.
PE   1: Evidence at protein level;
KW   Cell membrane; Membrane; Reference proteome; Sporulation;
KW   Toxin-antitoxin system; Transmembrane; Transmembrane helix.
FT   CHAIN           1..245
FT                   /note="Stage II sporulation protein SA"
FT                   /id="PRO_0000415544"
FT   TRANSMEM        2..22
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        43..63
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        66..86
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   245 AA;  28307 MW;  6B6F7FBC9589FF80 CRC64;
     MISNIRIGLF VLAIVFVVLV FFYWKNEELY EEKKQRIRKT WYGLFIISVT VYFMIKGIDL
     TLWKNLLMFT AMVIFVDIAF ILTPNISEIW GAKFSDIGKT VQSIKRSLIA SKARGEIYTT
     IIQNVNPAVF GTMEWHTEEE YTKSLNAFLD SYGEKIGAKI VVFEAAKELN TNFRGIRSQF
     SIIVPLEHIE QLNEQKAVQV ENVGIIPAKI VSDVFIVIDG KKNNLQDRDF ENVYNLTIHH
     SYFSK