SOX_ARATH
ID SOX_ARATH Reviewed; 416 AA.
AC Q9SJA7;
DT 23-JAN-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 136.
DE RecName: Full=Probable sarcosine oxidase;
DE EC=1.5.3.1;
GN OrderedLocusNames=At2g24580; ORFNames=F25P17.12;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + O2 + sarcosine = formaldehyde + glycine + H2O2;
CC Xref=Rhea:RHEA:13313, ChEBI:CHEBI:15377, ChEBI:CHEBI:15379,
CC ChEBI:CHEBI:16240, ChEBI:CHEBI:16842, ChEBI:CHEBI:57305,
CC ChEBI:CHEBI:57433; EC=1.5.3.1;
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692; Evidence={ECO:0000250};
CC Note=Binds 1 FAD per subunit. {ECO:0000250};
CC -!- SIMILARITY: Belongs to the MSOX/MTOX family. {ECO:0000305}.
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DR EMBL; AC006954; AAD23888.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC07594.1; -; Genomic_DNA.
DR EMBL; AY065254; AAL38730.1; -; mRNA.
DR EMBL; AY117247; AAM51322.1; -; mRNA.
DR PIR; D84638; D84638.
DR RefSeq; NP_180034.1; NM_128019.4.
DR AlphaFoldDB; Q9SJA7; -.
DR SMR; Q9SJA7; -.
DR BioGRID; 2346; 1.
DR IntAct; Q9SJA7; 1.
DR STRING; 3702.AT2G24580.1; -.
DR PaxDb; Q9SJA7; -.
DR PRIDE; Q9SJA7; -.
DR ProteomicsDB; 245331; -.
DR EnsemblPlants; AT2G24580.1; AT2G24580.1; AT2G24580.
DR GeneID; 816994; -.
DR Gramene; AT2G24580.1; AT2G24580.1; AT2G24580.
DR KEGG; ath:AT2G24580; -.
DR Araport; AT2G24580; -.
DR TAIR; locus:2046643; AT2G24580.
DR eggNOG; KOG2820; Eukaryota.
DR HOGENOM; CLU_007884_2_2_1; -.
DR InParanoid; Q9SJA7; -.
DR OMA; EMYSDPV; -.
DR OrthoDB; 752680at2759; -.
DR PhylomeDB; Q9SJA7; -.
DR BioCyc; ARA:AT2G24580-MON; -.
DR PRO; PR:Q9SJA7; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q9SJA7; baseline and differential.
DR Genevisible; Q9SJA7; AT.
DR GO; GO:0050660; F:flavin adenine dinucleotide binding; IEA:InterPro.
DR GO; GO:0008115; F:sarcosine oxidase activity; IDA:TAIR.
DR Gene3D; 3.50.50.60; -; 1.
DR InterPro; IPR006076; FAD-dep_OxRdtase.
DR InterPro; IPR036188; FAD/NAD-bd_sf.
DR InterPro; IPR045170; MTOX.
DR InterPro; IPR006281; SoxA_mon.
DR PANTHER; PTHR10961; PTHR10961; 1.
DR Pfam; PF01266; DAO; 1.
DR SUPFAM; SSF51905; SSF51905; 1.
DR TIGRFAMs; TIGR01377; soxA_mon; 1.
PE 2: Evidence at transcript level;
KW FAD; Flavoprotein; Oxidoreductase; Reference proteome.
FT CHAIN 1..416
FT /note="Probable sarcosine oxidase"
FT /id="PRO_0000213777"
FT BINDING 10..40
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000255"
FT MOD_RES 325
FT /note="S-8alpha-FAD cysteine"
FT /evidence="ECO:0000305"
SQ SEQUENCE 416 AA; 45702 MW; E12E3792DF305BCC CRC64;
MEYSDDGRFD VIVVGAGVMG SSAAYQLAKR GQKTLLLEQF DFLHHRGSSH GESRTIRATY
PEDYYYSMVS ESTRLWAAAQ SEIGYKVHFP TQQFDMGPAD QQSLLSVVAT CQKHGLAHRV
MDSHAVSEHF SGRISIPENW IGVSTELGGI IKPTKAVSMF QTLAIGHGAI LRDNTKVANI
KRDGESGEGV IVCTVKGDKF YGKKCIVTAG AWISKLVKTV AGIDFPVEPL ETTVCYWRIK
EGHEEKFTID GEFPTFASYG APYVYGTPSL EYPGLIKVAV HGGYWCDPDK RPWGPGVKLE
ELKEWIKERF GGMVDSEGPV ATQLCMYSMT PDEDFVIDFL GGEFGRDVVV GGGFSGHGFK
MAPAVGRILA DMAMEVEAGG GGVEMKQFSL RRFEDNPKGN AKEYPDQVIL DVPLKH
