SOXA_RHIME
ID SOXA_RHIME Reviewed; 987 AA.
AC O87386;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 30-MAY-2000, sequence version 2.
DT 25-MAY-2022, entry version 129.
DE RecName: Full=Sarcosine oxidase subunit alpha;
DE Short=Sarcosine oxidase subunit;
DE EC=1.5.3.1;
GN Name=soxA; OrderedLocusNames=R00083; ORFNames=SMc02606;
OS Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium
OS meliloti).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium.
OX NCBI_TaxID=266834;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=1021;
RX PubMed=11481430; DOI=10.1073/pnas.161294398;
RA Capela D., Barloy-Hubler F., Gouzy J., Bothe G., Ampe F., Batut J.,
RA Boistard P., Becker A., Boutry M., Cadieu E., Dreano S., Gloux S.,
RA Godrie T., Goffeau A., Kahn D., Kiss E., Lelaure V., Masuy D., Pohl T.,
RA Portetelle D., Puehler A., Purnelle B., Ramsperger U., Renard C.,
RA Thebault P., Vandenbol M., Weidner S., Galibert F.;
RT "Analysis of the chromosome sequence of the legume symbiont Sinorhizobium
RT meliloti strain 1021.";
RL Proc. Natl. Acad. Sci. U.S.A. 98:9877-9882(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=1021;
RX PubMed=11474104; DOI=10.1126/science.1060966;
RA Galibert F., Finan T.M., Long S.R., Puehler A., Abola P., Ampe F.,
RA Barloy-Hubler F., Barnett M.J., Becker A., Boistard P., Bothe G.,
RA Boutry M., Bowser L., Buhrmester J., Cadieu E., Capela D., Chain P.,
RA Cowie A., Davis R.W., Dreano S., Federspiel N.A., Fisher R.F., Gloux S.,
RA Godrie T., Goffeau A., Golding B., Gouzy J., Gurjal M., Hernandez-Lucas I.,
RA Hong A., Huizar L., Hyman R.W., Jones T., Kahn D., Kahn M.L., Kalman S.,
RA Keating D.H., Kiss E., Komp C., Lelaure V., Masuy D., Palm C., Peck M.C.,
RA Pohl T.M., Portetelle D., Purnelle B., Ramsperger U., Surzycki R.,
RA Thebault P., Vandenbol M., Vorhoelter F.J., Weidner S., Wells D.H.,
RA Wong K., Yeh K.-C., Batut J.;
RT "The composite genome of the legume symbiont Sinorhizobium meliloti.";
RL Science 293:668-672(2001).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-59.
RC STRAIN=1021;
RA Powers E.L., Vuyyuru V., Kahn M.L.;
RL Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Catalyzes the oxidative demethylation of sarcosine to yield
CC glycine, hydrogen peroxide and 5,10-methylenetetrahydrofolate.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + O2 + sarcosine = formaldehyde + glycine + H2O2;
CC Xref=Rhea:RHEA:13313, ChEBI:CHEBI:15377, ChEBI:CHEBI:15379,
CC ChEBI:CHEBI:16240, ChEBI:CHEBI:16842, ChEBI:CHEBI:57305,
CC ChEBI:CHEBI:57433; EC=1.5.3.1;
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692;
CC -!- SUBCELLULAR LOCATION: Cytoplasm.
CC -!- SIMILARITY: Belongs to the GcvT family. {ECO:0000305}.
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DR EMBL; AL591688; CAC41470.1; -; Genomic_DNA.
DR EMBL; AF055582; AAC62216.1; -; Genomic_DNA.
DR RefSeq; NP_384189.1; NC_003047.1.
DR RefSeq; WP_010968344.1; NC_003047.1.
DR AlphaFoldDB; O87386; -.
DR SMR; O87386; -.
DR STRING; 266834.SMc02606; -.
DR EnsemblBacteria; CAC41470; CAC41470; SMc02606.
DR GeneID; 61601564; -.
DR KEGG; sme:SMc02606; -.
DR PATRIC; fig|266834.11.peg.1440; -.
DR eggNOG; COG0404; Bacteria.
DR eggNOG; COG0446; Bacteria.
DR HOGENOM; CLU_011963_0_0_5; -.
DR OMA; GYGHVEL; -.
DR Proteomes; UP000001976; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0008115; F:sarcosine oxidase activity; IEA:UniProtKB-EC.
DR GO; GO:0046653; P:tetrahydrofolate metabolic process; IEA:InterPro.
DR Gene3D; 3.10.20.440; -; 1.
DR Gene3D; 3.30.1360.120; -; 1.
DR Gene3D; 3.50.50.60; -; 1.
DR InterPro; IPR042204; 2Fe-2S-bd_N.
DR InterPro; IPR036188; FAD/NAD-bd_sf.
DR InterPro; IPR023753; FAD/NAD-binding_dom.
DR InterPro; IPR028896; GCST/YgfZ/DmdA.
DR InterPro; IPR013977; GCV_T_C.
DR InterPro; IPR006222; GCV_T_N.
DR InterPro; IPR029043; GcvT/YgfZ_C.
DR InterPro; IPR006277; Sarcosine_oxidase_asu.
DR InterPro; IPR041117; SoxA_A3.
DR InterPro; IPR027266; TrmE/GcvT_dom1.
DR PANTHER; PTHR43757; PTHR43757; 1.
DR Pfam; PF01571; GCV_T; 1.
DR Pfam; PF08669; GCV_T_C; 1.
DR Pfam; PF07992; Pyr_redox_2; 1.
DR Pfam; PF17806; SO_alpha_A3; 1.
DR PIRSF; PIRSF037980; SoxA; 1.
DR SUPFAM; SSF101790; SSF101790; 1.
DR SUPFAM; SSF51905; SSF51905; 1.
DR TIGRFAMs; TIGR01372; soxA; 1.
PE 3: Inferred from homology;
KW Cytoplasm; FAD; Flavoprotein; Oxidoreductase; Reference proteome.
FT CHAIN 1..987
FT /note="Sarcosine oxidase subunit alpha"
FT /id="PRO_0000122628"
SQ SEQUENCE 987 AA; 105803 MW; FE811D96C8FABB31 CRC64;
MSSYRLPKRG LVDRNVPLSF TFDGRPMQGL EGDTLASALL ANGRMLVGRS FKYHRPRGIL
TAGAAEPNAL VTVGRGGRAE PNTRATMQEL YEGLEARSQN RWPSLAFDIG ALNGLLSPFL
GAGFYYKTFM WPAPLWEKLY EPVIRRAAGL GKASYEADPD AYEKSWAHCD LLVIGAGPTG
LAAALTAGRA GARVILVDEG SLPGGSLLSD TATIDGKAAA DFARDTSDEL RSMPNVQVLV
RTTAFGWYDG NVFGAVERVQ KHVREPASHL PVERLWRIVA GKALLATGAE ERPLVFGGND
RPGVMMAGAM RAYLNRYGVA PGRTPAIFTT NDTGYTLAQE LEAAGVDVVA IVDSRPAAGV
DYRGKARLVR EAVVCGTKGG KAISAIEVHH GGRTETIAVD ALAMAGGFDP IIHLACHRGG
KPVWSAEKAA FLAPGSLKGL EVAGGAAATT GLAACLGEGA ARAEAIVREL GLPCPPVAVV
KVESEEGIRS PAPLWSIPGI KDKAFVDFQN DVHLKDIGLA VREGYSHVEL AKRYTTSGMA
TDQGKLSNVN AIGLIAKARG VSPAEVGTTT FRPFYTPVSF GALTGAHTGH HFQPVRKSPL
HDWAKKHGAV FVETGLWYRS SWFPRSGERT WRESVEREVL NVRKNAGLCD VSMLGKIEIT
GSDAAEFLNR VYCNAFLKLP VGKARYGLML REDGFIYDDG TTSRLEENRF FMTTTTAYAA
GVMNHLEFCA QVLWPQLDVR LASITDQWAQ MAIAGPKARM ILQKIVDEDI SDAAFPFLAA
KEVSLFGGAL HGCLFRISFS GELAYELAVP AGYGESIADA LLEAGKDHGI MPYGVETLSV
LRIEKGHVTH NEINGTIVPA DLGFGKMVSA GKPDFVGKAM LQREGLTAPD RPQLVGVVPL
DPQQSFRSGS HILAKGAAAT LENDEGYVTS SAYSPHVGST IALALVRNGR NRHGEEVLVW
SGLHGESTPA RLCNPVFFDP QNERLHV
