SKFH_BACSU
ID SKFH_BACSU Reviewed; 141 AA.
AC O31430; Q7DL59;
DT 04-DEC-2007, integrated into UniProtKB/Swiss-Prot.
DT 16-JUN-2009, sequence version 2.
DT 03-AUG-2022, entry version 111.
DE RecName: Full=Thioredoxin-like protein SkfH;
GN Name=skfH {ECO:0000303|PubMed:12817086}; Synonyms=ybdE;
GN OrderedLocusNames=BSU01980;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RA Haga K., Liu H., Yasumoto K., Takahashi H., Yoshikawa H.;
RT "Sequence analysis of the 70kb region between 17 and 23 degree of the
RT Bacillus subtilis chromosome.";
RL Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [3]
RP SEQUENCE REVISION TO 4; 47 AND 130.
RX PubMed=19383706; DOI=10.1099/mic.0.027839-0;
RA Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A.,
RA Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.;
RT "From a consortium sequence to a unified sequence: the Bacillus subtilis
RT 168 reference genome a decade later.";
RL Microbiology 155:1758-1775(2009).
RN [4]
RP FUNCTION IN SYNTHESIS OF SKFA, INDUCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=168 / PY79;
RX PubMed=12817086; DOI=10.1126/science.1086462;
RA Gonzalez-Pastor J.E., Hobbs E.C., Losick R.;
RT "Cannibalism by sporulating bacteria.";
RL Science 301:510-513(2003).
RN [5]
RP REPRESSION BY ABRB AND ABH.
RX PubMed=17720793; DOI=10.1128/jb.01081-07;
RA Strauch M.A., Bobay B.G., Cavanagh J., Yao F., Wilson A., Le Breton Y.;
RT "Abh and AbrB control of Bacillus subtilis antimicrobial gene expression.";
RL J. Bacteriol. 189:7720-7732(2007).
CC -!- FUNCTION: Required for production of the bacteriocin SkfA.
CC {ECO:0000269|PubMed:12817086}.
CC -!- INDUCTION: By Spo0A (PubMed:12817086) and PhoP, during nutrient
CC starvation, especially phosphate starvation. Repressed by AbrB during
CC normal growth when nutrients are plentiful, in association with the
CC transcriptional repressor Abh. {ECO:0000269|PubMed:12817086,
CC ECO:0000269|PubMed:17720793}.
CC -!- DISRUPTION PHENOTYPE: When the skfA-skfB-skfC-skfE-skfF-skfG-skfH
CC operon is deleted, increased rate of spore formation; a double operon
CC deletion (sdpA-sdpC plus skfA-skfH) makes spores even faster
CC (PubMed:12817086). {ECO:0000269|PubMed:12817086}.
CC -!- MISCELLANEOUS: Accelerated cannibalism by skf- cells is seen on solid
CC media but not in liquid media. {ECO:0000269|PubMed:12817086}.
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DR EMBL; AB006424; BAA33095.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB11992.2; -; Genomic_DNA.
DR PIR; C69747; C69747.
DR RefSeq; NP_388080.2; NC_000964.3.
DR RefSeq; WP_003246390.1; NZ_JNCM01000030.1.
DR AlphaFoldDB; O31430; -.
DR SMR; O31430; -.
DR STRING; 224308.BSU01980; -.
DR PaxDb; O31430; -.
DR PRIDE; O31430; -.
DR EnsemblBacteria; CAB11992; CAB11992; BSU_01980.
DR GeneID; 938499; -.
DR KEGG; bsu:BSU01980; -.
DR PATRIC; fig|224308.179.peg.204; -.
DR eggNOG; COG0526; Bacteria.
DR PhylomeDB; O31430; -.
DR BioCyc; BSUB:BSU01980-MON; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0030152; P:bacteriocin biosynthetic process; IEA:UniProtKB-KW.
DR InterPro; IPR012336; Thioredoxin-like_fold.
DR InterPro; IPR036249; Thioredoxin-like_sf.
DR Pfam; PF13905; Thioredoxin_8; 1.
DR SUPFAM; SSF52833; SSF52833; 1.
PE 1: Evidence at protein level;
KW Antibiotic biosynthesis; Bacteriocin biosynthesis; Disulfide bond;
KW Redox-active center; Reference proteome.
FT CHAIN 1..141
FT /note="Thioredoxin-like protein SkfH"
FT /id="PRO_0000312743"
FT DOMAIN 2..141
FT /note="Thioredoxin"
FT DISULFID 41..44
FT /note="Redox-active"
FT /evidence="ECO:0000250"
FT CONFLICT 4
FT /note="E -> G (in Ref. 1; BAA33095)"
FT /evidence="ECO:0000305"
FT CONFLICT 47
FT /note="L -> F (in Ref. 1; BAA33095)"
FT /evidence="ECO:0000305"
FT CONFLICT 130
FT /note="G -> S (in Ref. 1; BAA33095)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 141 AA; 16230 MW; 9B355918728835A5 CRC64;
MKDEQMLTEW PSHLPWLNQS QNDFTFPSDT YLLLYFWSMS CPNCHQLTDK VLQDIKDMNV
KVIGVHVPYI EEEKSMEVVL TYALDRGLAI PIVLDQNYEI VTTCHVQGIP SFCLLSQYGQ
IITKTMGDVG WDKMLKKIAG L
