ID   SEPF_HALVD              Reviewed;         118 AA.
AC   D4H018; A0A384LGM0; L9UK63;
DT   23-FEB-2022, integrated into UniProtKB/Swiss-Prot.
DT   18-MAY-2010, sequence version 1.
DT   03-AUG-2022, entry version 55.
DE   RecName: Full=Cell division protein SepF {ECO:0000305};
GN   Name=sepF {ECO:0000303|PubMed:34103513, ECO:0000312|EMBL:ADE03140.1};
GN   OrderedLocusNames=HVO_0392 {ECO:0000312|EMBL:ADE03140.1};
GN   ORFNames=C498_16738 {ECO:0000312|EMBL:ELY25041.1};
OS   Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 /
OS   NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii).
OC   Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Haloferacales;
OC   Haloferacaceae; Haloferax.
OX   NCBI_TaxID=309800 {ECO:0000312|EMBL:ADE03140.1};
RN   [1] {ECO:0000312|EMBL:ADE03140.1, ECO:0000312|Proteomes:UP000008243}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM
RC   B-1768 / DS2 {ECO:0000312|Proteomes:UP000008243};
RX   PubMed=20333302; DOI=10.1371/journal.pone.0009605;
RA   Hartman A.L., Norais C., Badger J.H., Delmas S., Haldenby S., Madupu R.,
RA   Robinson J., Khouri H., Ren Q., Lowe T.M., Maupin-Furlow J.,
RA   Pohlschroder M., Daniels C., Pfeiffer F., Allers T., Eisen J.A.;
RT   "The complete genome sequence of Haloferax volcanii DS2, a model
RT   archaeon.";
RL   PLoS ONE 5:E9605-E9605(2010).
RN   [2] {ECO:0000312|EMBL:ELY25041.1, ECO:0000312|Proteomes:UP000011532}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM
RC   B-1768 / DS2 {ECO:0000312|Proteomes:UP000011532};
RX   PubMed=25393412; DOI=10.1371/journal.pgen.1004784;
RA   Becker E.A., Seitzer P.M., Tritt A., Larsen D., Krusor M., Yao A.I., Wu D.,
RA   Madern D., Eisen J.A., Darling A.E., Facciotti M.T.;
RT   "Phylogenetically driven sequencing of extremely halophilic archaea reveals
RT   strategies for static and dynamic osmo-response.";
RL   PLoS Genet. 10:E1004784-E1004784(2014).
RN   [3]
RP   FUNCTION, SUBUNIT, INTERACTION WITH FTSZ2, SUBCELLULAR LOCATION, DISRUPTION
RP   PHENOTYPE, AND REGION.
RC   STRAIN=ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM
RC   B-1768 / DS2 {ECO:0000303|PubMed:34103513};
RX   PubMed=34103513; DOI=10.1038/s41467-021-23686-9;
RA   Nussbaum P., Gerstner M., Dingethal M., Erb C., Albers S.V.;
RT   "The archaeal protein SepF is essential for cell division in Haloferax
RT   volcanii.";
RL   Nat. Commun. 12:3469-3469(2021).
CC   -!- FUNCTION: Involved in cell division. Probably acts as a membrane anchor
CC       for FstZ2, tethering its filaments to the division site. May be
CC       involved in septum closure. {ECO:0000269|PubMed:34103513}.
CC   -!- SUBUNIT: Homodimer. Does not oligomerize. Interacts with FtsZ2.
CC       {ECO:0000269|PubMed:34103513}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:34103513}.
CC       Note=Localizes in a ring-like structure at the cell division plane at
CC       midcell. Localizes at the future site of cell division straight after
CC       the closure of the septum. Co-localizes with FtsZ1 and FtsZ2. FtsZ1 is
CC       important for its proper localization. {ECO:0000269|PubMed:34103513}.
CC   -!- DISRUPTION PHENOTYPE: Depletion of this protein leads to formation of
CC       large cells with a cragged cell surface, which are unable to establish
CC       a cell division plane. {ECO:0000269|PubMed:34103513}.
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DR   EMBL; CP001956; ADE03140.1; -; Genomic_DNA.
DR   EMBL; AOHU01000102; ELY25041.1; -; Genomic_DNA.
DR   RefSeq; WP_004044531.1; NZ_AOHU01000102.1.
DR   SMR; D4H018; -.
DR   STRING; 309800.C498_16738; -.
DR   EnsemblBacteria; ADE03140; ADE03140; HVO_0392.
DR   EnsemblBacteria; ELY25041; ELY25041; C498_16738.
DR   GeneID; 8924204; -.
DR   KEGG; hvo:HVO_0392; -.
DR   PATRIC; fig|309800.29.peg.3244; -.
DR   eggNOG; arCOG02263; Archaea.
DR   HOGENOM; CLU_131897_0_0_2; -.
DR   OMA; MGIMSKI; -.
DR   OrthoDB; 99949at2157; -.
DR   Proteomes; UP000008243; Chromosome.
DR   Proteomes; UP000011532; Unassembled WGS sequence.
DR   GO; GO:0032153; C:cell division site; IDA:UniProtKB.
DR   GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR   GO; GO:0042802; F:identical protein binding; IDA:UniProtKB.
DR   GO; GO:0042803; F:protein homodimerization activity; IDA:UniProtKB.
DR   GO; GO:0051301; P:cell division; IMP:UniProtKB.
DR   GO; GO:0000917; P:division septum assembly; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.110.150; -; 1.
DR   InterPro; IPR007561; Cell_div_SepF/SepF-rel.
DR   InterPro; IPR038594; SepF-like_sf.
DR   InterPro; IPR012426; SepF_arc.
DR   Pfam; PF04472; SepF; 1.
DR   PIRSF; PIRSF019313; UCP019313; 1.
PE   1: Evidence at protein level;
KW   Cell cycle; Cell division; Cytoplasm; Reference proteome; Septation.
FT   CHAIN           1..118
FT                   /note="Cell division protein SepF"
FT                   /id="PRO_0000454725"
FT   REGION          1..12
FT                   /note="Important for localization in a ring-like structure
FT                   at midcell"
FT                   /evidence="ECO:0000269|PubMed:34103513"
SQ   SEQUENCE   118 AA;  12640 MW;  9A13BEEB8F2362D5 CRC64;
     MGIMSKILGG GGSRTTEDYV ELDLDDFDTA RGDAGLSVHI AEIGGQQDVI AIKDAVYDGN
     LVIADITRHT TSDSTMEHII DDLRQVAREV DGDIVQKGDD QIVITPTGIS ISRQKLNG