SCW_DROME
ID SCW_DROME Reviewed; 400 AA.
AC P54631; Q8INV8;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 02-NOV-2001, sequence version 2.
DT 03-AUG-2022, entry version 174.
DE RecName: Full=Protein screw;
DE Flags: Precursor;
GN Name=scw; ORFNames=CG31695;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, AND DEVELOPMENTAL
RP STAGE.
RC STRAIN=Canton-S;
RX PubMed=7958918; DOI=10.1101/gad.8.21.2588;
RA Arora K., Levine M.S., O'Connor M.B.;
RT "The screw gene encodes a ubiquitously expressed member of the TGF-beta
RT family required for specification of dorsal cell fates in the Drosophila
RT embryo.";
RL Genes Dev. 8:2588-2601(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
CC -!- FUNCTION: Part of the signal that specifies dorsal cell fates in the
CC embryo. Acts together with dpp. {ECO:0000269|PubMed:7958918}.
CC -!- SUBUNIT: Heterodimers of scw/dpp are the active subunit, dpp/dpp
CC homodimers elicit a basal response and scw/scw homodimers alone are
CC ineffective in specifying a dorsal pattern.
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- TISSUE SPECIFICITY: Ubiquitously expressed during early stages of
CC embryogenesis, but the effect on development appears graded and is
CC restricted to the dorsal side of the embryo.
CC {ECO:0000269|PubMed:7958918}.
CC -!- DEVELOPMENTAL STAGE: Expressed both maternally and zygotically. Highest
CC embryonic expression is found during syncytial blastoderm (nuclear
CC cycles 11-12). Expression declines rapidly at cellular blastoderm stage
CC 5 and is not detected during the rest of embryonic development.
CC {ECO:0000269|PubMed:7958918}.
CC -!- SIMILARITY: Belongs to the TGF-beta family. {ECO:0000305}.
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DR EMBL; U17573; AAA56872.1; -; mRNA.
DR EMBL; AE014134; AAN11056.2; -; Genomic_DNA.
DR EMBL; AY051793; AAK93217.1; -; mRNA.
DR RefSeq; NP_001286088.1; NM_001299159.1.
DR RefSeq; NP_524863.3; NM_080124.5.
DR AlphaFoldDB; P54631; -.
DR BioGRID; 70052; 7.
DR DIP; DIP-59820N; -.
DR IntAct; P54631; 2.
DR STRING; 7227.FBpp0080802; -.
DR GlyGen; P54631; 5 sites.
DR PaxDb; P54631; -.
DR DNASU; 46000; -.
DR EnsemblMetazoa; FBtr0081261; FBpp0080802; FBgn0005590.
DR EnsemblMetazoa; FBtr0343860; FBpp0310402; FBgn0005590.
DR GeneID; 46000; -.
DR KEGG; dme:Dmel_CG31695; -.
DR UCSC; CG31695-RA; d. melanogaster.
DR CTD; 46000; -.
DR FlyBase; FBgn0005590; scw.
DR VEuPathDB; VectorBase:FBgn0005590; -.
DR eggNOG; KOG3900; Eukaryota.
DR HOGENOM; CLU_020515_4_1_1; -.
DR InParanoid; P54631; -.
DR OMA; FEAYFCG; -.
DR OrthoDB; 1063560at2759; -.
DR PhylomeDB; P54631; -.
DR SignaLink; P54631; -.
DR BioGRID-ORCS; 46000; 0 hits in 3 CRISPR screens.
DR GenomeRNAi; 46000; -.
DR PRO; PR:P54631; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0005590; Expressed in ectoderm anlage and 15 other tissues.
DR ExpressionAtlas; P54631; baseline and differential.
DR Genevisible; P54631; DM.
DR GO; GO:0005615; C:extracellular space; IDA:FlyBase.
DR GO; GO:0005125; F:cytokine activity; IBA:GO_Central.
DR GO; GO:0008083; F:growth factor activity; IEA:UniProtKB-KW.
DR GO; GO:0046982; F:protein heterodimerization activity; IPI:UniProtKB.
DR GO; GO:0048018; F:receptor ligand activity; IDA:FlyBase.
DR GO; GO:0007378; P:amnioserosa formation; IMP:FlyBase.
DR GO; GO:0030509; P:BMP signaling pathway; IDA:FlyBase.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0007502; P:digestive tract mesoderm development; IMP:FlyBase.
DR GO; GO:0009953; P:dorsal/ventral pattern formation; IMP:FlyBase.
DR GO; GO:0008406; P:gonad development; IMP:FlyBase.
DR GO; GO:0007476; P:imaginal disc-derived wing morphogenesis; IMP:FlyBase.
DR GO; GO:0010862; P:positive regulation of pathway-restricted SMAD protein phosphorylation; IBA:GO_Central.
DR GO; GO:0060395; P:SMAD protein signal transduction; IBA:GO_Central.
DR Gene3D; 2.10.90.10; -; 1.
DR InterPro; IPR029034; Cystine-knot_cytokine.
DR InterPro; IPR001839; TGF-b_C.
DR InterPro; IPR001111; TGF-b_propeptide.
DR InterPro; IPR015615; TGF-beta-rel.
DR InterPro; IPR017948; TGFb_CS.
DR PANTHER; PTHR11848; PTHR11848; 1.
DR Pfam; PF00019; TGF_beta; 1.
DR Pfam; PF00688; TGFb_propeptide; 1.
DR SMART; SM00204; TGFB; 1.
DR SUPFAM; SSF57501; SSF57501; 1.
DR PROSITE; PS00250; TGF_BETA_1; 1.
DR PROSITE; PS51362; TGF_BETA_2; 1.
PE 2: Evidence at transcript level;
KW Cleavage on pair of basic residues; Developmental protein; Differentiation;
KW Disulfide bond; Glycoprotein; Growth factor; Reference proteome; Secreted;
KW Signal.
FT SIGNAL 1..16
FT /evidence="ECO:0000255"
FT PROPEP 17..277
FT /evidence="ECO:0000255"
FT /id="PRO_0000033672"
FT CHAIN 278..400
FT /note="Protein screw"
FT /id="PRO_0000033673"
FT CARBOHYD 165
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 189
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 201
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 304
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 342
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 300..365
FT /evidence="ECO:0000250"
FT DISULFID 329..397
FT /evidence="ECO:0000250"
FT DISULFID 333..399
FT /evidence="ECO:0000250"
FT DISULFID 364
FT /note="Interchain"
FT /evidence="ECO:0000250"
FT CONFLICT 6
FT /note="F -> L (in Ref. 1; AAA56872)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 400 AA; 45891 MW; 2B42B7D28B9B8336 CRC64;
MLNVFFLTSL FYAASATTYV TTNNHIEMPI YQKRPLSEQM EMIDILDLGD RPRRQAEPNL
HNSASKFLLE VYNEISEDQE PKEVLHQRHK RSLDDDILIS NEDRQEIASC NSILTFSSRL
KPEQLDNELD MHITFNTNDV PVDLSLVQAM LRIYKQPSLV DRRANFTVSV YRKLDNRQDF
SYRILGSVNT TSSQRGWLEF NLTDTLRYWL HNKGLQRRNE LRISIGDSQL STFAAGLVTP
QASRTSLEPF IVGYFNGPEL LVKIQKLRFK RDLEKRRAGG GSPPPPPPPP VDLYRPPQSC
ERLNFTVDFK ELHMHNWVIA PKKFEAYFCG GGCNFPLGTK MNATNHAIVQ TLMHLKQPHL
PKPCCVPTVL GAITILRYLN EDIIDLTKYQ KAVAKECGCH
