SCTG_YEREN
ID SCTG_YEREN Reviewed; 131 AA.
AC Q56851; Q56923;
DT 18-SEP-2019, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 25-MAY-2022, entry version 77.
DE RecName: Full=Type 3 secretion system pilotin {ECO:0000305};
DE AltName: Full=Lipoprotein VirG {ECO:0000305};
DE AltName: Full=Pilot protein YscW {ECO:0000303|PubMed:15292137};
DE Flags: Precursor;
GN Name=yscW {ECO:0000303|PubMed:15292137};
GN Synonyms=sctG {ECO:0000305}, virG {ECO:0000303|PubMed:7635810};
GN ORFNames=ERS137951_03726 {ECO:0000312|EMBL:CNK32226.1};
OS Yersinia enterocolitica.
OG Plasmid pYVa127/90, Plasmid pYVe227, and Plasmid pYVe8081.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Yersiniaceae; Yersinia.
OX NCBI_TaxID=630;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBCELLULAR LOCATION, DISRUPTION
RP PHENOTYPE, AND MUTAGENESIS OF CYS-121 AND CYS-125.
RC STRAIN=W22703 / Serotype O:9 / Biotype 2; PLASMID=pYVe227;
RX PubMed=7635810; DOI=10.1128/jb.177.15.4230-4237.1995;
RA Allaoui A., Scheen R., Lambert de Rouvroit C., Cornelis G.R.;
RT "VirG, a Yersinia enterocolitica lipoprotein involved in Ca2+ dependency,
RT is related to exsB of Pseudomonas aeruginosa.";
RL J. Bacteriol. 177:4230-4237(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=W22703 / Serotype O:9 / Biotype 2; PLASMID=pYVe227;
RA Iriarte M., Lambermont I., Kerbourch C., Cornelis G.R.;
RT "Detailed genetic map of the pYVe227 plasmid of Yersinia enterocolitica
RT serotype O:9.";
RL Submitted (OCT-1998) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=8081; PLASMID=pYVe8081;
RX PubMed=11402007; DOI=10.1128/iai.69.7.4627-4638.2001;
RA Snellings N.J., Popek M., Lindler L.E.;
RT "Complete DNA sequence of Yersinia enterocolitica serotype 0:8 low-calcium-
RT response plasmid reveals a new virulence plasmid-associated replicon.";
RL Infect. Immun. 69:4627-4638(2001).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=A127/90 / Serotype O:8 / Biotype 1B; PLASMID=pYVa127/90;
RX PubMed=14527656; DOI=10.1016/s0923-2508(03)00147-5;
RA Foultier B., Cornelis G.R.;
RT "DNA sequence and analysis of the pYVa127/90 virulence plasmid of Yersinia
RT enterocolitica strain A127/90.";
RL Res. Microbiol. 154:553-557(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=WA;
RG Pathogen Informatics;
RA Murphy D.;
RL Submitted (MAR-2015) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=W22703 / Serotype O:9 / Biotype 2;
RX PubMed=9427408; DOI=10.1046/j.1365-2958.1997.6141981.x;
RA Koster M., Bitter W., de Cock H., Allaoui A., Cornelis G.R., Tommassen J.;
RT "The outer membrane component, YscC, of the Yop secretion machinery of
RT Yersinia enterocolitica forms a ring-shaped multimeric complex.";
RL Mol. Microbiol. 26:789-797(1997).
RN [7]
RP FUNCTION, INTERACTION WITH YSCC/SCTC, SUBCELLULAR LOCATION, AND DISRUPTION
RP PHENOTYPE.
RC STRAIN=W22703 / Serotype O:9 / Biotype 2;
RX PubMed=15292137; DOI=10.1128/jb.186.16.5366-5375.2004;
RA Burghout P., Beckers F., de Wit E., van Boxtel R., Cornelis G.R.,
RA Tommassen J., Koster M.;
RT "Role of the pilot protein YscW in the biogenesis of the YscC secretin in
RT Yersinia enterocolitica.";
RL J. Bacteriol. 186:5366-5375(2004).
CC -!- FUNCTION: Involved in the synthesis of the type III secretion system
CC (T3SS), also called injectisome, which is used to inject bacterial
CC effector proteins into eukaryotic host cells (PubMed:9427408,
CC PubMed:15292137). Pilot protein that is required for the proper
CC localization of the secretin YscC/SctC in the outer membrane
CC (PubMed:9427408, PubMed:15292137). Also required for efficient
CC oligomerization of YscC/SctC and stabilization of the oligomers
CC (PubMed:15292137). {ECO:0000269|PubMed:15292137,
CC ECO:0000269|PubMed:9427408}.
CC -!- SUBUNIT: Interacts with YscC/SctC. {ECO:0000269|PubMed:15292137}.
CC -!- SUBCELLULAR LOCATION: Cell outer membrane
CC {ECO:0000269|PubMed:15292137}; Lipid-anchor {ECO:0000255|PROSITE-
CC ProRule:PRU00303, ECO:0000269|PubMed:7635810}. Note=Association with
CC the outer membrane is a prerequisite for function in the biogenesis of
CC YscC/SctC. {ECO:0000269|PubMed:15292137}.
CC -!- DISRUPTION PHENOTYPE: In mutant, only a small amount of YscC/SctC is
CC properly targeted to the outer membrane (PubMed:9427408,
CC PubMed:15292137). Mutant is Ca(2+) independent for growth at 37 degrees
CC Celsius and secretes smaller amounts of Yop proteins (PubMed:7635810).
CC {ECO:0000269|PubMed:15292137, ECO:0000269|PubMed:7635810,
CC ECO:0000269|PubMed:9427408}.
CC -!- SIMILARITY: Belongs to the ExsB/YscW family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AF102990; AAD16832.1; -; Genomic_DNA.
DR EMBL; AF336309; AAK69231.1; -; Genomic_DNA.
DR EMBL; AY150843; AAN37559.1; -; Genomic_DNA.
DR EMBL; CQCV01000031; CNK32226.1; -; Genomic_DNA.
DR PIR; A32242; A32242.
DR RefSeq; NP_052409.1; NC_002120.1.
DR RefSeq; NP_783683.1; NC_004564.1.
DR RefSeq; NP_863531.1; NC_005017.1.
DR RefSeq; WP_005176443.1; NZ_SJZK01000009.1.
DR AlphaFoldDB; Q56851; -.
DR SMR; Q56851; -.
DR EnsemblBacteria; CNK32226; CNK32226; ERS137951_03726.
DR PATRIC; fig|630.129.peg.4370; -.
DR OrthoDB; 1781915at2; -.
DR GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR InterPro; IPR039366; Pilotin.
DR Pfam; PF09619; YscW; 1.
DR PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE 1: Evidence at protein level;
KW Cell outer membrane; Lipoprotein; Membrane; Palmitate; Plasmid; Signal;
KW Virulence.
FT SIGNAL 1..15
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT CHAIN 16..131
FT /note="Type 3 secretion system pilotin"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT /id="PRO_0000447818"
FT LIPID 16
FT /note="N-palmitoyl cysteine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT LIPID 16
FT /note="S-diacylglycerol cysteine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT MUTAGEN 121
FT /note="C->S: No change in activity."
FT /evidence="ECO:0000269|PubMed:7635810"
FT MUTAGEN 125
FT /note="C->S: No change in activity."
FT /evidence="ECO:0000269|PubMed:7635810"
SQ SEQUENCE 131 AA; 14687 MW; 140EB83FB145CECA CRC64;
MSRIIALIIS FLLVGCATPP MPAQRIVGEV RMSRPLSRTA HIDVSIFGLY EGKVREVQRT
RFETGNLPLF FSIKLNPAQR GEGELYLRST LSFPERGVQA VAQQKLIGKN KVVLQMIPKT
CYPNCQSPNT R
