ID   SCP_APLCA               Reviewed;         136 AA.
AC   P09892;
DT   01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1989, sequence version 1.
DT   25-MAY-2022, entry version 55.
DE   RecName: Full=Small cardioactive peptides;
DE   Contains:
DE     RecName: Full=Small cardioactive peptide B;
DE              Short=SCP B;
DE   Contains:
DE     RecName: Full=Small cardioactive peptide A;
DE              Short=SCP A;
DE   Flags: Precursor;
OS   Aplysia californica (California sea hare).
OC   Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Gastropoda;
OC   Heterobranchia; Euthyneura; Tectipleura; Aplysiida; Aplysioidea;
OC   Aplysiidae; Aplysia.
OX   NCBI_TaxID=6500;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND AMIDATION AT MET-33 AND MET-46.
RX   PubMed=3858852; DOI=10.1073/pnas.82.11.3925;
RA   Mahon A.C., Lloyd P.E., Weiss K.R., Kupfermann I., Scheller R.H.;
RT   "The small cardioactive peptides A and B of Aplysia are derived from a
RT   common precursor molecule.";
RL   Proc. Natl. Acad. Sci. U.S.A. 82:3925-3929(1985).
RN   [2]
RP   MASS SPECTROMETRY.
RX   PubMed=9809658; DOI=10.1016/s0196-9781(98)00094-1;
RA   Li L., Moroz T.P., Garden R.W., Floyd P.D., Weiss K.R., Sweedler J.V.;
RT   "Mass spectrometric survey of interganglionically transported peptides in
RT   Aplysia.";
RL   Peptides 19:1425-1433(1998).
RN   [3]
RP   IDENTIFICATION BY MASS SPECTROMETRY.
RX   PubMed=10959975; DOI=10.1021/ac000260z;
RA   Li L., Romanova E.V., Rubakhin S.S., Alexeeva V., Weiss K.R., Vilim F.S.,
RA   Sweedler J.V.;
RT   "Peptide profiling of cells with multiple gene products: combining
RT   immunochemistry and MALDI mass spectrometry with on-plate
RT   microextraction.";
RL   Anal. Chem. 72:3867-3874(2000).
CC   -!- FUNCTION: Involved in the stimulation of contractile activity in the
CC       gut, the increase of the amplitude of the heart beat, and enhancement
CC       of the contractile response of the radula closer muscle.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Highly expressed in the buccal ganglion.
CC   -!- PTM: Contains three disulfide bonds.
CC   -!- MASS SPECTROMETRY: [Small cardioactive peptide B]: Mass=1140.8;
CC       Mass_error=0.3; Method=MALDI; Evidence={ECO:0000269|PubMed:9809658};
CC   -!- MASS SPECTROMETRY: [Small cardioactive peptide A]: Mass=1277.0;
CC       Mass_error=0.7; Method=MALDI; Evidence={ECO:0000269|PubMed:9809658};
CC   -!- SIMILARITY: Belongs to the SCP family. {ECO:0000305}.
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DR   EMBL; M11396; AAA27777.1; -; mRNA.
DR   RefSeq; XP_012943902.1; XM_013088448.1.
DR   AlphaFoldDB; P09892; -.
DR   SMR; P09892; -.
DR   GeneID; 101845040; -.
DR   OrthoDB; 1840082at2759; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0007218; P:neuropeptide signaling pathway; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Amidation; Disulfide bond; Neuropeptide; Secreted; Signal.
FT   SIGNAL          1..24
FT                   /evidence="ECO:0000255"
FT   PEPTIDE         25..33
FT                   /note="Small cardioactive peptide B"
FT                   /id="PRO_0000001877"
FT   PEPTIDE         36..46
FT                   /note="Small cardioactive peptide A"
FT                   /id="PRO_0000311917"
FT   PROPEP          49..135
FT                   /note="Carboxy-terminal peptide"
FT                   /id="PRO_0000311918"
FT   MOD_RES         33
FT                   /note="Methionine amide"
FT                   /evidence="ECO:0000269|PubMed:3858852"
FT   MOD_RES         46
FT                   /note="Methionine amide"
FT                   /evidence="ECO:0000269|PubMed:3858852"
SQ   SEQUENCE   136 AA;  14773 MW;  8988DB25670CC1EE CRC64;
     METSVSRVTV SLTLLVLIIC SADAMNYLAF PRMGRARPGY LAFPRMGRSQ MKTETGTDCC
     GLGMKSEFVI GQEGKEELRH GACSSSVACC AGLREIVDQK QDGVFFSMCV PDFVASRSSE
     ESSSEVLSKL KSLLQK