SCP1_BRALA
ID SCP1_BRALA Reviewed; 185 AA.
AC P04569;
DT 13-AUG-1987, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1990, sequence version 2.
DT 03-AUG-2022, entry version 86.
DE RecName: Full=Sarcoplasmic calcium-binding proteins I, III, and IV;
DE Short=SCP I, III, IV;
OS Branchiostoma lanceolatum (Common lancelet) (Amphioxus lanceolatum).
OC Eukaryota; Metazoa; Chordata; Cephalochordata; Leptocardii; Amphioxiformes;
OC Branchiostomidae; Branchiostoma.
OX NCBI_TaxID=7740;
RN [1]
RP PROTEIN SEQUENCE (SCP I).
RA Takagi T., Konishi K., Cox J.A.;
RT "Amino acid sequence of two sarcoplasmic calcium-binding proteins from the
RT protochordate amphioxus.";
RL Biochemistry 25:3585-3592(1986).
RN [2]
RP PROTEIN SEQUENCE (SCP III AND IV), AND SEQUENCE REVISION (SCP I).
RX PubMed=2209593; DOI=10.1111/j.1432-1033.1990.tb19239.x;
RA Takagi T., Cox J.A.;
RT "Amino acid sequences of four isoforms of amphioxus sarcoplasmic calcium-
RT binding proteins.";
RL Eur. J. Biochem. 192:387-399(1990).
CC -!- FUNCTION: Like parvalbumins, SCPs seem to be more abundant in fast
CC contracting muscles, but no functional relationship can be established
CC from this distribution.
CC -!- MISCELLANEOUS: The sarcoplasmic calcium-binding proteins are abundant
CC in the muscle of arthropods, mollusks, annelids, and protochordates.
CC -!- MISCELLANEOUS: This protein has three functional calcium-binding sites;
CC potential site 4 has lost affinity for calcium.
CC -!- MISCELLANEOUS: There are 7 different SCP's in amphioxus. The sequence
CC shown here is that of SCP I.
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DR PIR; B24479; B24479.
DR PIR; S13184; S13184.
DR PIR; S13185; S13185.
DR AlphaFoldDB; P04569; -.
DR SMR; P04569; -.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR InterPro; IPR002048; EF_hand_dom.
DR Pfam; PF13202; EF-hand_5; 1.
DR Pfam; PF13499; EF-hand_7; 1.
DR SUPFAM; SSF47473; SSF47473; 1.
DR PROSITE; PS00018; EF_HAND_1; 3.
DR PROSITE; PS50222; EF_HAND_2; 3.
PE 1: Evidence at protein level;
KW Calcium; Direct protein sequencing; Metal-binding; Muscle protein; Repeat.
FT CHAIN 1..185
FT /note="Sarcoplasmic calcium-binding proteins I, III, and
FT IV"
FT /id="PRO_0000073632"
FT DOMAIN 5..41
FT /note="EF-hand 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 57..92
FT /note="EF-hand 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 102..137
FT /note="EF-hand 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 138..173
FT /note="EF-hand 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 19
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 21
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 23
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 25
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 30
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 70
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 72
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 74
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 81
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 115
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 117
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 119
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 126
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT VARIANT 20
FT /note="Y -> M (in SCP III)"
FT VARIANT 23
FT /note="D -> N (in SCP IV)"
SQ SEQUENCE 185 AA; 21418 MW; 92D556A663003FAD CRC64;
GLNDFQKQKI KFTFDFFLDY NKDGSIQWED FEEMIKRYKE VNKGSLSDAD YKSMQASLED
EWRDLKGRAD INKDDVVSWE EYLAMWEKTI ATCKSVADLP AWCQNRIPFL FKGMDVSGDG
IVDLEEFQNY CKNFQLQCAD VPAVYNVITD GGKVTFDLNR YKELYYRLLT SPAADAGNTL
MGQKP
