SCOB_BACSU
ID SCOB_BACSU Reviewed; 216 AA.
AC P42316;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 1.
DT 03-AUG-2022, entry version 127.
DE RecName: Full=Probable succinyl-CoA:3-ketoacid coenzyme A transferase subunit B;
DE EC=2.8.3.5;
DE AltName: Full=OXCT B;
DE AltName: Full=Succinyl-CoA:3-oxoacid CoA-transferase;
GN Name=scoB; Synonyms=yxjE; OrderedLocusNames=BSU38980; ORFNames=N15L;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168 / BGSC1A1;
RX PubMed=8969509; DOI=10.1099/13500872-142-11-3113;
RA Yoshida K., Shindo K., Sano H., Seki S., Fujimura M., Yanai N., Miwa Y.,
RA Fujita Y.;
RT "Sequencing of a 65 kb region of the Bacillus subtilis genome containing
RT the lic and cel loci, and creation of a 177 kb contig covering the gnt-
RT sacXY region.";
RL Microbiology 142:3113-3123(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 3-oxo acid + succinyl-CoA = a 3-oxoacyl-CoA + succinate;
CC Xref=Rhea:RHEA:24564, ChEBI:CHEBI:30031, ChEBI:CHEBI:35973,
CC ChEBI:CHEBI:57292, ChEBI:CHEBI:90726; EC=2.8.3.5;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU10034};
CC -!- SUBUNIT: Heterodimer of a subunit A and a subunit B. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the 3-oxoacid CoA-transferase subunit B family.
CC {ECO:0000305}.
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DR EMBL; D83026; BAA11706.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB15924.1; -; Genomic_DNA.
DR PIR; C70079; C70079.
DR RefSeq; NP_391777.1; NC_000964.3.
DR RefSeq; WP_003243049.1; NZ_JNCM01000034.1.
DR PDB; 3CDK; X-ray; 2.59 A; B/D=1-216.
DR PDBsum; 3CDK; -.
DR AlphaFoldDB; P42316; -.
DR SMR; P42316; -.
DR STRING; 224308.BSU38980; -.
DR PaxDb; P42316; -.
DR PRIDE; P42316; -.
DR DNASU; 937453; -.
DR EnsemblBacteria; CAB15924; CAB15924; BSU_38980.
DR GeneID; 937453; -.
DR KEGG; bsu:BSU38980; -.
DR PATRIC; fig|224308.179.peg.4218; -.
DR eggNOG; COG2057; Bacteria.
DR InParanoid; P42316; -.
DR OMA; ANWRVGS; -.
DR PhylomeDB; P42316; -.
DR BioCyc; BSUB:BSU38980-MON; -.
DR EvolutionaryTrace; P42316; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0008260; F:3-oxoacid CoA-transferase activity; IEA:UniProtKB-EC.
DR GO; GO:0008775; F:acetate CoA-transferase activity; IBA:GO_Central.
DR GO; GO:0008410; F:CoA-transferase activity; IBA:GO_Central.
DR InterPro; IPR012791; 3-oxoacid_CoA-transf_B.
DR InterPro; IPR004165; CoA_trans_fam_I.
DR InterPro; IPR004164; CoA_transf_AS.
DR InterPro; IPR037171; NagB/RpiA_transferase-like.
DR PANTHER; PTHR13707; PTHR13707; 1.
DR Pfam; PF01144; CoA_trans; 1.
DR SMART; SM00882; CoA_trans; 1.
DR SUPFAM; SSF100950; SSF100950; 1.
DR TIGRFAMs; TIGR02428; pcaJ_scoB_fam; 1.
DR PROSITE; PS01274; COA_TRANSF_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Reference proteome; Transferase.
FT CHAIN 1..216
FT /note="Probable succinyl-CoA:3-ketoacid coenzyme A
FT transferase subunit B"
FT /id="PRO_0000157920"
FT ACT_SITE 47
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10034"
FT HELIX 2..13
FT /evidence="ECO:0007829|PDB:3CDK"
FT STRAND 21..24
FT /evidence="ECO:0007829|PDB:3CDK"
FT HELIX 28..33
FT /evidence="ECO:0007829|PDB:3CDK"
FT STRAND 43..46
FT /evidence="ECO:0007829|PDB:3CDK"
FT TURN 47..49
FT /evidence="ECO:0007829|PDB:3CDK"
FT STRAND 50..54
FT /evidence="ECO:0007829|PDB:3CDK"
FT TURN 59..61
FT /evidence="ECO:0007829|PDB:3CDK"
FT STRAND 71..73
FT /evidence="ECO:0007829|PDB:3CDK"
FT STRAND 75..82
FT /evidence="ECO:0007829|PDB:3CDK"
FT HELIX 85..93
FT /evidence="ECO:0007829|PDB:3CDK"
FT STRAND 99..102
FT /evidence="ECO:0007829|PDB:3CDK"
FT STRAND 105..108
FT /evidence="ECO:0007829|PDB:3CDK"
FT HELIX 129..135
FT /evidence="ECO:0007829|PDB:3CDK"
FT STRAND 136..142
FT /evidence="ECO:0007829|PDB:3CDK"
FT STRAND 152..157
FT /evidence="ECO:0007829|PDB:3CDK"
FT STRAND 163..166
FT /evidence="ECO:0007829|PDB:3CDK"
FT STRAND 169..172
FT /evidence="ECO:0007829|PDB:3CDK"
FT STRAND 174..181
FT /evidence="ECO:0007829|PDB:3CDK"
FT STRAND 184..190
FT /evidence="ECO:0007829|PDB:3CDK"
FT HELIX 196..201
FT /evidence="ECO:0007829|PDB:3CDK"
SQ SEQUENCE 216 AA; 23331 MW; 2D0E402D7A62C0F5 CRC64;
MKEARKRMVK RAVQEIKDGM NVNLGIGMPT LVANEIPDGV HVMLQSENGL LGIGPYPLEG
TEDADLINAG KETITEVTGA SYFDSAESFA MIRGGHIDLA ILGGMEVSEQ GDLANWMIPG
KMVKGMGGAM DLVNGAKRIV VIMEHVNKHG ESKVKKTCSL PLTGQKVVHR LITDLAVFDF
VNGRMTLTEL QDGVTIEEVY EKTEADFAVS QSVLNS
