SAT10_STACB
ID SAT10_STACB Reviewed; 1930 AA.
AC A0A084B9Z8;
DT 22-NOV-2017, integrated into UniProtKB/Swiss-Prot.
DT 29-OCT-2014, sequence version 1.
DT 03-AUG-2022, entry version 24.
DE RecName: Full=Ankyrin repeat domain-containing protein SAT10 {ECO:0000303|PubMed:25015739};
DE AltName: Full=Satratoxin biosynthesis SC1 cluster protein 10 {ECO:0000303|PubMed:25015739};
GN Name=SAT10 {ECO:0000303|PubMed:25015739}; ORFNames=S7711_07284;
OS Stachybotrys chartarum (strain CBS 109288 / IBT 7711) (Toxic black mold)
OS (Stilbospora chartarum).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Hypocreomycetidae; Hypocreales; Stachybotryaceae; Stachybotrys.
OX NCBI_TaxID=1280523;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], IDENTIFICATION, AND
RP FUNCTION.
RC STRAIN=CBS 109288 / IBT 7711;
RX PubMed=25015739; DOI=10.1186/1471-2164-15-590;
RA Semeiks J., Borek D., Otwinowski Z., Grishin N.V.;
RT "Comparative genome sequencing reveals chemotype-specific gene clusters in
RT the toxigenic black mold Stachybotrys.";
RL BMC Genomics 15:590-590(2014).
CC -!- FUNCTION: Ankyrin repeat domain-containing protein; part of the
CC satratoxin SC1 cluster involved in the biosynthesis of satratoxins,
CC trichothecene mycotoxins that are associated with human food poisonings
CC (PubMed:25015739). Satratoxins are suggested to be made by products of
CC multiple gene clusters (SC1, SC2 and SC3) that encode 21 proteins in
CC all, including polyketide synthases, acetyltransferases, and other
CC enzymes expected to modify the trichothecene skeleton
CC (PubMed:25015739). SC1 encodes 10 proteins, SAT1 to SAT10
CC (PubMed:25015739). The largest are SAT8, which encodes a putative
CC polyketide synthase (PKS) with a conventional non-reducing
CC architecture, and SAT10, a putative protein containing four ankyrin
CC repeats and thus may be involved in protein scaffolding
CC (PubMed:25015739). The putative short-chain reductase SAT3 may assist
CC the PKS in some capacity (PubMed:25015739). SAT6 contains a secretory
CC lipase domain and acts probably as a trichothecene esterase
CC (PubMed:25015739). SAT5 encodes a putative acetyltransferase, and so,
CC with SAT6, may affect endogenous protection from toxicity
CC (PubMed:25015739). The probable transcription factor SAT9 may regulate
CC the expression of the SC1 cluster (PubMed:25015739). SC2 encodes
CC proteins SAT11 to SAT16, the largest of which encodes the putative
CC reducing PKS SAT13 (PubMed:25015739). SAT11 is a cytochrome P450
CC monooxygenase, while SAT14 and SAT16 are probable acetyltransferases
CC (PubMed:25015739). The SC2 cluster may be regulated by the
CC transcription factor SAT15 (PubMed:25015739). SC3 is a small cluster
CC that encodes 5 proteins, SAT17 to SAT21 (PubMed:25015739). SAT21 is a
CC putative MFS-type transporter which may have a role in exporting
CC secondary metabolites (PubMed:25015739). The four other proteins
CC putatively encoded in SC3 include the taurine hydroxylase-like protein
CC SAT17, the O-methyltransferase SAT18, the acetyltransferase SAT19, and
CC the Cys6-type zinc finger SAT20, the latter being probably involved in
CC regulation of SC3 expression (PubMed:25015739).
CC {ECO:0000305|PubMed:25015739}.
CC -!- PATHWAY: Mycotoxin biosynthesis. {ECO:0000269|PubMed:25015739}.
CC -!- MISCELLANEOUS: Trichothecenes are sesquiterpenoid toxins that act by
CC inhibiting protein biosynthesis. {ECO:0000305}.
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DR EMBL; KL647604; KEY74377.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A084B9Z8; -.
DR SMR; A0A084B9Z8; -.
DR EnsemblFungi; KEY74377; KEY74377; S7711_07284.
DR HOGENOM; CLU_002405_1_0_1; -.
DR OrthoDB; 1115202at2759; -.
DR Proteomes; UP000028045; Unassembled WGS sequence.
DR Gene3D; 1.25.40.20; -; 4.
DR InterPro; IPR002110; Ankyrin_rpt.
DR InterPro; IPR036770; Ankyrin_rpt-contain_sf.
DR Pfam; PF12796; Ank_2; 3.
DR SMART; SM00248; ANK; 18.
DR SUPFAM; SSF48403; SSF48403; 3.
DR PROSITE; PS50297; ANK_REP_REGION; 1.
DR PROSITE; PS50088; ANK_REPEAT; 3.
PE 4: Predicted;
KW ANK repeat; Repeat.
FT CHAIN 1..1930
FT /note="Ankyrin repeat domain-containing protein SAT10"
FT /id="PRO_0000442416"
FT REPEAT 840..872
FT /note="ANK 1"
FT /evidence="ECO:0000255"
FT REPEAT 878..908
FT /note="ANK 2"
FT /evidence="ECO:0000255"
FT REPEAT 920..949
FT /note="ANK 3"
FT /evidence="ECO:0000255"
FT REPEAT 959..989
FT /note="ANK 4"
FT /evidence="ECO:0000255"
FT REPEAT 993..1023
FT /note="ANK 5"
FT /evidence="ECO:0000255"
FT REPEAT 1073..1102
FT /note="ANK 6"
FT /evidence="ECO:0000255"
FT REPEAT 1106..1135
FT /note="ANK 7"
FT /evidence="ECO:0000255"
FT REPEAT 1144..1174
FT /note="ANK 8"
FT /evidence="ECO:0000255"
FT REPEAT 1178..1205
FT /note="ANK 9"
FT /evidence="ECO:0000255"
FT REPEAT 1206..1235
FT /note="ANK 10"
FT /evidence="ECO:0000255"
FT REPEAT 1239..1268
FT /note="ANK 11"
FT /evidence="ECO:0000255"
FT REPEAT 1272..1301
FT /note="ANK 12"
FT /evidence="ECO:0000255"
FT REPEAT 1304..1333
FT /note="ANK 13"
FT /evidence="ECO:0000255"
FT REPEAT 1339..1368
FT /note="ANK 14"
FT /evidence="ECO:0000255"
FT REPEAT 1400..1429
FT /note="ANK 15"
FT /evidence="ECO:0000255"
FT REPEAT 1514..1543
FT /note="ANK 16"
FT /evidence="ECO:0000255"
FT REPEAT 1548..1577
FT /note="ANK 17"
FT /evidence="ECO:0000255"
FT REPEAT 1615..1644
FT /note="ANK 18"
FT /evidence="ECO:0000255"
FT REPEAT 1651..1680
FT /note="ANK 19"
FT /evidence="ECO:0000255"
FT REPEAT 1696..1724
FT /note="ANK 20"
FT /evidence="ECO:0000255"
SQ SEQUENCE 1930 AA; 213700 MW; 9B56B4BC4B4EA1EA CRC64;
MSIQFFSYDV GGLIVKEVRL LILWRRSALP NGRTLFARMT QALRIASSDK KYRSIFDRTS
LLAFFATPHR STQHQSPESV ALALLNQCYC GLISPWISIF PPNFSKVVAR SEVEFRPPTR
AHILNVFQDP GPASPIKDSV VVHKSCAVLG VDGEVLIGLD CSHYTLARLL KARDKRYLLR
QASHAALRHG KAFQQVVGLF FLDSIRTFDA EGPKFECRKV VSQLASLPQL QSWRQGSVDS
RVLWFGTPAM LDPTSLFRTL RSQIQEENQL GDPIFIRVDS TLHRHNELSE PQILASMCQQ
ILRQQPQLTS ALQDLLLNVE DAAVGSRDCW KQRTLWNCLL VLLYHPKDAE TFCFIDATSS
LQTKNLAGQL ESVMKESEMP LRLIVSCRST AKQTPEASTQ VDVDLSDAGF DEPLRQDLEE
WIRESLECGL TDSTLREALL TQILSSGDFH LARHALEFFA STGSWLTKWS VPSVWAMLSK
QSAAELFIED NIRRHGQWLL IPMLWALEAF EPMQVDEIDV ALMLEDNGVA GQVEDFINLL
PGISTIRRGV FVIADHVRPA FDYLWGKYFA TYQHHVYLAK SCVAALRHHL RVTPAIPQLR
EDKSSDAAAR LCTYAAMNWV RHFSLQRNSE TTKYVPHPTI ITANETDPGS PNEHAGVSEA
FLEDPELSRL WITHLRRALD LDGLDEELGH LILPETLGSR LGICTGWAIR ISRQLASMRL
SSERDVTNSL LVIGSETDDL AMVQSCLSAD PSPTEHTLGY ALAAASDPIK EKLLQQVGEP
SDEFLYRALL SSICFGNVSV TEDLLVRITD KVRVAQVTPL EGSKLQWPQA NESSESAETF
RHTPLGVATA YGDADVIDLL INHDISWWDL EERSPPPGTW NALHHAALGG QRNIMCKLLL
QQRKGVLNSS ARIPNTVTES GNTPLILAAS RGFHKIVALL LEDGSMRGYG VDVNIQNEQR
SSALLAAARY GFSQTLEMLL TYEGIDYSKT DSNGASILHL ALVNDREAAA LQILAHKDIF
SNEMEYQEAN MEANSVNNFE DDDSFSESSV DTTDVVYTVP ARPRISLHQK DGSGLTSLTI
AIWRNLKSIV EILIAMDADA NGPEGEFEAP LVAAAEVGSF ELFTMFTKIG ATKTEAALNT
ISTGRTRPLH AACAMGHLEV VRELLKDSVT QLSHTDSNQR TPLCAAISRD QNHVISVLLD
RETETGLQEG LWEAARSGKA HILDQLLRRG AEINAQDEYG NTALQWASYY NKPRCVERLL
LGGARLDLLD CDNVNALGDA ARSGSAEPLK LLVDVGVDVN AEAGGDTALC RAIWAEEVEC
VSVLLQGGAK FILSSAQSRF ENLLTFAVQV SSPEILRLLL KAPEERDLAP TLRSACAMQS
TSQLEVLLEF YDPAKVDLGS GWTILHLAAV HGTLAGLTKV LDHATGRAAL NYGPKKVGTP
FEMAAFSSKE SLSKVEHLYS NAALPGLVQP SSRFGTALNA ASYRLNDPVV VYLLQKMQLE
DINASGARYG NAIQNMLASA WMDTERSLKL LGILLEAGVS LTPTSADRHG TALHTAALFS
PKPLVEKVIE TSRMLADERD GEGRLPIHLS ALQEEWASMM LLSTTTSTIR SVDKMGRNAV
HLAAAAGARS VLEKIFEVEE NEDLLLEADF DGWTPFHWAC RGEDDDCARF LIEKARKIFD
SKWDSMKHEL VTTDEKTWTP LDVARFHQRR EVELLLSLGM TTSDAENWMP DQSQNLGSYC
DSCACQIWHE EHHSSALHCK KLYLRFNGWS RMCQLAPTAK RKYTPTLLGY KTCFKVADNL
VKARLAKVLV PHSQGPLQRK GRGKGEGEED EEGIATVENY PVSQSCGVEM IRAFVIDDRR
IQPDATRNLN HEVSVGSEDQ TQMSLGTGRL FVQSVDSPLE MVMDVAAIFL SSPKSAHKQD
ETTRAFATAK
