ID   SART_STAA8              Reviewed;         118 AA.
AC   Q2G2B1;
DT   05-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT   05-SEP-2006, sequence version 2.
DT   25-MAY-2022, entry version 90.
DE   RecName: Full=HTH-type transcriptional regulator SarT;
DE   AltName: Full=Staphylococcal accessory regulator T;
GN   Name=sarT; OrderedLocusNames=SAOUHSC_02799;
OS   Staphylococcus aureus (strain NCTC 8325 / PS 47).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=93061;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NCTC 8325 / PS 47;
RA   Gillaspy A.F., Worrell V., Orvis J., Roe B.A., Dyer D.W., Iandolo J.J.;
RT   "The Staphylococcus aureus NCTC 8325 genome.";
RL   (In) Fischetti V., Novick R., Ferretti J., Portnoy D., Rood J. (eds.);
RL   Gram positive pathogens, 2nd edition, pp.381-412, ASM Press, Washington
RL   D.C. (2006).
RN   [2]
RP   PROTEIN SEQUENCE OF 1-9, REPRESSION BY SARA AND AGR, AND REGULATION OF HLA.
RX   PubMed=11447147; DOI=10.1128/iai.69.8.4749-4758.2001;
RA   Schmidt K.A., Manna A.C., Gill S.R., Cheung A.L.;
RT   "SarT, a repressor of alpha-hemolysin in Staphylococcus aureus.";
RL   Infect. Immun. 69:4749-4758(2001).
RN   [3]
RP   REGULATION OF SARU AND AGR.
RX   PubMed=12496184; DOI=10.1128/iai.71.1.343-353.2003;
RA   Manna A.C., Cheung A.L.;
RT   "sarU, a sarA homolog, is repressed by SarT and regulates virulence genes
RT   in Staphylococcus aureus.";
RL   Infect. Immun. 71:343-353(2003).
RN   [4]
RP   REGULATION OF SARS AND SPA.
RX   PubMed=12933857; DOI=10.1128/iai.71.9.5139-5148.2003;
RA   Schmidt K.A., Manna A.C., Cheung A.L.;
RT   "SarT influences sarS expression in Staphylococcus aureus.";
RL   Infect. Immun. 71:5139-5148(2003).
CC   -!- FUNCTION: Transcriptional regulator acting as an intermediary between
CC       major regulators sarA and agr and virulence genes. Represses alpha-
CC       hemolysin (hla) gene expression. Down-regulates agr RNAIII expression
CC       by repressing sarU, a positive activator of agr expression. Up-
CC       regulates sarS, which induces the expression of the cell wall-
CC       associated protein A (spa).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- INDUCTION: Repressed by SarA and agr, particularly in the post-
CC       exponential phase. {ECO:0000269|PubMed:11447147}.
CC   -!- SIMILARITY: Belongs to the SarA family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=ABD31802.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; CP000253; ABD31802.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; WP_000998869.1; NZ_LS483365.1.
DR   RefSeq; YP_501258.1; NC_007795.1.
DR   AlphaFoldDB; Q2G2B1; -.
DR   SMR; Q2G2B1; -.
DR   STRING; 1280.SAXN108_2745; -.
DR   EnsemblBacteria; ABD31802; ABD31802; SAOUHSC_02799.
DR   GeneID; 3921453; -.
DR   KEGG; sao:SAOUHSC_02799; -.
DR   PATRIC; fig|93061.5.peg.2531; -.
DR   eggNOG; COG1846; Bacteria.
DR   HOGENOM; CLU_2095348_0_0_9; -.
DR   Proteomes; UP000008816; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IBA:GO_Central.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR000835; HTH_MarR-typ.
DR   InterPro; IPR010166; SarA/Rot.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   Pfam; PF13463; HTH_27; 1.
DR   SUPFAM; SSF46785; SSF46785; 1.
DR   TIGRFAMs; TIGR01889; Staph_reg_Sar; 1.
PE   1: Evidence at protein level;
KW   Activator; Cytoplasm; Direct protein sequencing; DNA-binding;
KW   Reference proteome; Repressor; Transcription; Transcription regulation;
KW   Virulence.
FT   CHAIN           1..118
FT                   /note="HTH-type transcriptional regulator SarT"
FT                   /id="PRO_0000249330"
FT   DNA_BIND        55..78
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   118 AA;  13988 MW;  231D70A48079ECF7 CRC64;
     MNDLKSKSNI KLMKRVLTTY ELRKYLKKYF CLTLDNYLVL AYLDVFKNDE GKYFMRDIIS
     YIGIDQSRIV KSVKDLSKKG YLNKCRDPHD SRNVIIVVSV KQHNYIKNLL SEININET