ID   S9AD_STAAN              Reviewed;         260 AA.
AC   P0A0D1; P04827;
DT   13-AUG-1987, integrated into UniProtKB/Swiss-Prot.
DT   13-AUG-1987, sequence version 1.
DT   25-MAY-2022, entry version 82.
DE   RecName: Full=Spectinomycin 9-adenylyltransferase;
DE   AltName: Full=AAD(9);
GN   Name=ant1; Synonyms=spc1; OrderedLocusNames=SA0049;
GN   and
GN   Name=ant2; Synonyms=spc2; OrderedLocusNames=SA1481;
GN   and
GN   Name=ant3; Synonyms=spc3; OrderedLocusNames=SA0765;
GN   and
GN   Name=ant4; Synonyms=spc4; OrderedLocusNames=SA1952;
GN   and
GN   Name=ant5; Synonyms=spc5; OrderedLocusNames=SA2385;
OS   Staphylococcus aureus (strain N315).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=158879;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=10348769; DOI=10.1128/aac.43.6.1449;
RA   Ito T., Katayama Y., Hiramatsu K.;
RT   "Cloning and nucleotide sequence determination of the entire mec DNA of
RT   pre-methicillin-resistant Staphylococcus aureus N315.";
RL   Antimicrob. Agents Chemother. 43:1449-1458(1999).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=N315;
RX   PubMed=11418146; DOI=10.1016/s0140-6736(00)04403-2;
RA   Kuroda M., Ohta T., Uchiyama I., Baba T., Yuzawa H., Kobayashi I., Cui L.,
RA   Oguchi A., Aoki K., Nagai Y., Lian J.-Q., Ito T., Kanamori M.,
RA   Matsumaru H., Maruyama A., Murakami H., Hosoyama A., Mizutani-Ui Y.,
RA   Takahashi N.K., Sawano T., Inoue R., Kaito C., Sekimizu K., Hirakawa H.,
RA   Kuhara S., Goto S., Yabuzaki J., Kanehisa M., Yamashita A., Oshima K.,
RA   Furuya K., Yoshino C., Shiba T., Hattori M., Ogasawara N., Hayashi H.,
RA   Hiramatsu K.;
RT   "Whole genome sequencing of meticillin-resistant Staphylococcus aureus.";
RL   Lancet 357:1225-1240(2001).
RN   [3]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=N315;
RA   Vaezzadeh A.R., Deshusses J., Lescuyer P., Hochstrasser D.F.;
RT   "Shotgun proteomic analysis of total and membrane protein extracts of S.
RT   aureus strain N315.";
RL   Submitted (OCT-2007) to UniProtKB.
CC   -!- FUNCTION: Mediates bacterial resistance to the antibiotic spectinomycin
CC       but not streptomycin. {ECO:0000250|UniProtKB:P0A0D2}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + spectinomycin = 9-O-adenylylspectinomycin + diphosphate;
CC         Xref=Rhea:RHEA:63228, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019,
CC         ChEBI:CHEBI:146260, ChEBI:CHEBI:146261;
CC         Evidence={ECO:0000250|UniProtKB:P0A0D2};
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DR   EMBL; D86934; BAA82204.1; -; Genomic_DNA.
DR   EMBL; BA000018; BAB41267.1; -; Genomic_DNA.
DR   EMBL; BA000018; BAB42002.1; -; Genomic_DNA.
DR   EMBL; BA000018; BAB42747.1; -; Genomic_DNA.
DR   EMBL; BA000018; BAB43236.1; -; Genomic_DNA.
DR   EMBL; BA000018; BAB43690.1; -; Genomic_DNA.
DR   RefSeq; WP_000067268.1; NC_002745.2.
DR   AlphaFoldDB; P0A0D1; -.
DR   SMR; P0A0D1; -.
DR   EnsemblBacteria; BAB41267; BAB41267; BAB41267.
DR   EnsemblBacteria; BAB42002; BAB42002; BAB42002.
DR   EnsemblBacteria; BAB42747; BAB42747; BAB42747.
DR   EnsemblBacteria; BAB43236; BAB43236; BAB43236.
DR   EnsemblBacteria; BAB43690; BAB43690; BAB43690.
DR   GeneID; 42043718; -.
DR   GeneID; 58051002; -.
DR   KEGG; sau:SA0049; -.
DR   KEGG; sau:SA0765; -.
DR   KEGG; sau:SA1481; -.
DR   KEGG; sau:SA1952; -.
DR   KEGG; sau:SA2385; -.
DR   HOGENOM; CLU_071584_0_1_9; -.
DR   OMA; VRPWRYP; -.
DR   Proteomes; UP000000751; Chromosome.
DR   GO; GO:0070566; F:adenylyltransferase activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.460.10; -; 1.
DR   InterPro; IPR024172; AadA/Aad9.
DR   InterPro; IPR025184; DUF4111.
DR   InterPro; IPR043519; NT_sf.
DR   InterPro; IPR002934; Polymerase_NTP_transf_dom.
DR   Pfam; PF13427; DUF4111; 1.
DR   Pfam; PF01909; NTP_transf_2; 1.
DR   PIRSF; PIRSF000819; Streptomycin_3-adenylyltransf; 1.
DR   SUPFAM; SSF81301; SSF81301; 1.
PE   1: Evidence at protein level;
KW   Antibiotic resistance; ATP-binding; Nucleotide-binding;
KW   Nucleotidyltransferase; Transferase; Transposable element.
FT   CHAIN           1..260
FT                   /note="Spectinomycin 9-adenylyltransferase"
FT                   /id="PRO_0000068585"
SQ   SEQUENCE   260 AA;  28975 MW;  3C821F312A108F54 CRC64;
     MSNLINGKIP NQAIQTLKIV KDLFGSSIVG VYLFGSAVNG GLRINSDVDV LVVVNHSLPQ
     LTRKKLTERL MTISGKIGNT DSVRPLEVTV INRSEVVPWQ YPPKREFIYG EWLRGEFENG
     QIQEPSYDPD LAIVLAQARK NSISLFGPDS SSILVSVPLT DIRRAIKDSL PELIEGIKGD
     ERNVILTLAR MWQTVTTGEI TSKDVAAEWA IPLLPKEHVT LLDIARKGYR GECDDKWEGL
     YSKVKALVKY MKNSIETSLN