S22A9_HUMAN
ID S22A9_HUMAN Reviewed; 553 AA.
AC Q8IVM8; A0AVB7; A4PB24; Q8TCC8; Q8TEC0; Q8WYN7;
DT 02-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 146.
DE RecName: Full=Solute carrier family 22 member 9;
DE AltName: Full=Organic anion transporter 7;
GN Name=SLC22A9; Synonyms=hOAT4, OAT7, UST3;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION,
RP TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC TISSUE=Liver;
RX PubMed=17393504; DOI=10.1002/hep.21596;
RA Shin H.J., Anzai N., Enomoto A., He X., Kim do K., Endou H., Kanai Y.;
RT "Novel liver-specific organic anion transporter OAT7 that operates the
RT exchange of sulfate conjugates for short chain fatty acid butyrate.";
RL Hepatology 45:1046-1055(2007).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC TISSUE=Kidney;
RA Engel K., Gruendemann D., Schoemig E.;
RT "Putative integral membrane transport protein UST3H.";
RL Submitted (NOV-2000) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC TISSUE=Fetal liver;
RA Takanaga H., Ohtsuki S., Hosoya K., Terasaki T.;
RT "Isolation of novel clone of amphiphilic solute facilitator family from
RT human fetal liver.";
RL Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RA Guo J.H., Yu L.;
RL Submitted (OCT-2001) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Hepatoma;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16554811; DOI=10.1038/nature04632;
RA Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K.,
RA Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T.,
RA Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G.,
RA Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C.,
RA Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A.,
RA Hattori M., Rogers J., Lander E.S., Sakaki Y.;
RT "Human chromosome 11 DNA sequence and analysis including novel gene
RT identification.";
RL Nature 440:497-500(2006).
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [8]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC TISSUE=Brain, and Liver;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [9]
RP IDENTIFICATION, AND TISSUE SPECIFICITY.
RX PubMed=11327718; DOI=10.1006/bbrc.2001.4774;
RA Sun W., Wu R.R., van Poelje P.D., Erion M.D.;
RT "Isolation of a family of organic anion transporters from human liver and
RT kidney.";
RL Biochem. Biophys. Res. Commun. 283:417-422(2001).
RN [10]
RP VARIANTS [LARGE SCALE ANALYSIS] VAL-393; VAL-487 AND LYS-521.
RX PubMed=16959974; DOI=10.1126/science.1133427;
RA Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D.,
RA Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P.,
RA Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V.,
RA Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H.,
RA Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W.,
RA Velculescu V.E.;
RT "The consensus coding sequences of human breast and colorectal cancers.";
RL Science 314:268-274(2006).
CC -!- FUNCTION: Sodium-independent organic anion transporter which exhibits
CC high specificity for sulfated conjugates of xenobiotics and steroid
CC hormones. It is also specifically activated by 3 to 5 carbons-
CC containing short-chain fatty acids/SCFAs, including propionate,
CC butyrate and valerate. May operate the exchange of sulfated organic
CC components against short-chain fatty acids/SCFAs at the sinusoidal
CC membrane of hepatocytes. {ECO:0000269|PubMed:17393504}.
CC -!- SUBCELLULAR LOCATION: Basolateral cell membrane
CC {ECO:0000269|PubMed:17393504}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:17393504}. Note=Enriched at the sinusoidal part of
CC the plasma membrane.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8IVM8-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8IVM8-2; Sequence=VSP_036710, VSP_036711;
CC -!- TISSUE SPECIFICITY: Specifically expressed in liver by hepatocytes (at
CC protein level). {ECO:0000269|PubMed:11327718,
CC ECO:0000269|PubMed:17393504}.
CC -!- DEVELOPMENTAL STAGE: Expressed in fetal liver.
CC {ECO:0000269|PubMed:17393504}.
CC -!- MISCELLANEOUS: [Isoform 2]: May be produced at very low levels due to a
CC premature stop codon in the mRNA, leading to nonsense-mediated mRNA
CC decay. {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the major facilitator (TC 2.A.1) superfamily.
CC Organic cation transporter (TC 2.A.1.19) family. {ECO:0000305}.
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DR EMBL; AB074812; BAF51552.1; -; mRNA.
DR EMBL; AJ295270; CAC82910.1; -; mRNA.
DR EMBL; AB062418; BAB83517.1; -; mRNA.
DR EMBL; AF440402; AAP97316.1; -; mRNA.
DR EMBL; AK074246; BAB85030.1; -; mRNA.
DR EMBL; AP001880; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AP000484; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471076; EAW74146.1; -; Genomic_DNA.
DR EMBL; CH471076; EAW74148.1; -; Genomic_DNA.
DR EMBL; BC022379; AAH22379.1; -; mRNA.
DR EMBL; BC126288; AAI26289.1; -; mRNA.
DR CCDS; CCDS8043.1; -. [Q8IVM8-1]
DR RefSeq; NP_543142.2; NM_080866.2. [Q8IVM8-1]
DR AlphaFoldDB; Q8IVM8; -.
DR SMR; Q8IVM8; -.
DR BioGRID; 125322; 148.
DR IntAct; Q8IVM8; 67.
DR MINT; Q8IVM8; -.
DR STRING; 9606.ENSP00000279178; -.
DR ChEMBL; CHEMBL2073721; -.
DR TCDB; 2.A.1.19.18; the major facilitator superfamily (mfs).
DR GlyGen; Q8IVM8; 3 sites.
DR iPTMnet; Q8IVM8; -.
DR PhosphoSitePlus; Q8IVM8; -.
DR BioMuta; SLC22A9; -.
DR DMDM; 74723638; -.
DR MassIVE; Q8IVM8; -.
DR PaxDb; Q8IVM8; -.
DR PeptideAtlas; Q8IVM8; -.
DR PRIDE; Q8IVM8; -.
DR ProteomicsDB; 70744; -. [Q8IVM8-1]
DR ProteomicsDB; 70745; -. [Q8IVM8-2]
DR Antibodypedia; 2750; 60 antibodies from 15 providers.
DR DNASU; 114571; -.
DR Ensembl; ENST00000279178.4; ENSP00000279178.3; ENSG00000149742.10. [Q8IVM8-1]
DR Ensembl; ENST00000536333.5; ENSP00000440206.1; ENSG00000149742.10. [Q8IVM8-2]
DR GeneID; 114571; -.
DR KEGG; hsa:114571; -.
DR MANE-Select; ENST00000279178.4; ENSP00000279178.3; NM_080866.3; NP_543142.2.
DR UCSC; uc001nww.4; human. [Q8IVM8-1]
DR CTD; 114571; -.
DR DisGeNET; 114571; -.
DR GeneCards; SLC22A9; -.
DR HGNC; HGNC:16261; SLC22A9.
DR HPA; ENSG00000149742; Tissue enriched (liver).
DR MIM; 607579; gene.
DR neXtProt; NX_Q8IVM8; -.
DR OpenTargets; ENSG00000149742; -.
DR PharmGKB; PA38102; -.
DR VEuPathDB; HostDB:ENSG00000149742; -.
DR eggNOG; KOG0255; Eukaryota.
DR GeneTree; ENSGT00940000161239; -.
DR HOGENOM; CLU_001265_33_0_1; -.
DR InParanoid; Q8IVM8; -.
DR OMA; WLIMTNK; -.
DR OrthoDB; 464838at2759; -.
DR PhylomeDB; Q8IVM8; -.
DR TreeFam; TF315847; -.
DR PathwayCommons; Q8IVM8; -.
DR SignaLink; Q8IVM8; -.
DR BioGRID-ORCS; 114571; 12 hits in 1056 CRISPR screens.
DR GeneWiki; SLC22A9; -.
DR GenomeRNAi; 114571; -.
DR Pharos; Q8IVM8; Tbio.
DR PRO; PR:Q8IVM8; -.
DR Proteomes; UP000005640; Chromosome 11.
DR RNAct; Q8IVM8; protein.
DR Bgee; ENSG00000149742; Expressed in right lobe of liver and 31 other tissues.
DR Genevisible; Q8IVM8; HS.
DR GO; GO:0016323; C:basolateral plasma membrane; IDA:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0015301; F:anion:anion antiporter activity; IDA:UniProtKB.
DR GO; GO:0015636; F:short-chain fatty acid transmembrane transporter activity; IDA:UniProtKB.
DR GO; GO:0015347; F:sodium-independent organic anion transmembrane transporter activity; IDA:UniProtKB.
DR GO; GO:0009914; P:hormone transport; IDA:UniProtKB.
DR GO; GO:0015711; P:organic anion transport; IBA:GO_Central.
DR GO; GO:0015913; P:short-chain fatty acid import; IDA:UniProtKB.
DR GO; GO:0043252; P:sodium-independent organic anion transport; IDA:UniProtKB.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR036259; MFS_trans_sf.
DR Pfam; PF07690; MFS_1; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR PROSITE; PS50850; MFS; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell membrane; Glycoprotein; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..553
FT /note="Solute carrier family 22 member 9"
FT /id="PRO_0000233715"
FT TOPO_DOM 1..15
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 16..36
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 37..143
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 144..164
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 165..177
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 178..198
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 199..203
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 204..224
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 225..233
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 234..254
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 255..259
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 260..280
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 281..350
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 351..369
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 370..378
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 379..399
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 400..407
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 408..428
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 429..436
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 437..457
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 458..469
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 470..490
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 491..495
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 496..516
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 517..553
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 528..553
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 528..547
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 39
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 56
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 102
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 169..346
FT /note="RFGRRFVLRWCYLQVAIVGTCAALAPTFLIYCSLRFLSGIAAMSLITNTIML
FT IAEWATHRFQAMGITLGMCPSGIAFMTLAGLAFAIRDWHILQLVVSVPYFVIFLTSSWL
FT LESARWLIINNKPEEGLKELRKAAHRSGMKNARDTLTLEILKSTMKKELEAAQKKKPSL
FT CEMLHMPN -> SSRVGNTQIPGHGNYIGNVPFWYCIYDPGRPGFCHSRLAYPPAGGVC
FT TILCDLSDLKLAARVCSVAHYQQ (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_036710"
FT VAR_SEQ 347..553
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_036711"
FT VARIANT 393
FT /note="A -> V (in a breast cancer sample; somatic
FT mutation)"
FT /evidence="ECO:0000269|PubMed:16959974"
FT /id="VAR_036403"
FT VARIANT 487
FT /note="M -> V (in a breast cancer sample; somatic
FT mutation)"
FT /evidence="ECO:0000269|PubMed:16959974"
FT /id="VAR_036404"
FT VARIANT 521
FT /note="N -> K (in a colorectal cancer sample; somatic
FT mutation; dbSNP:rs770073076)"
FT /evidence="ECO:0000269|PubMed:16959974"
FT /id="VAR_036405"
FT CONFLICT 518
FT /note="E -> G (in Ref. 3; BAB83517)"
FT /evidence="ECO:0000305"
FT CONFLICT 535
FT /note="Missing (in Ref. 5; BAB85030)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 553 AA; 62169 MW; FE0A1B725FCB7617 CRC64;
MAFQDLLGHA GDLWRFQILQ TVFLSIFAVA TYLHFMLENF TAFIPGHRCW VHILDNDTVS
DNDTGALSQD ALLRISIPLD SNMRPEKCRR FVHPQWQLLH LNGTFPNTSD ADMEPCVDGW
VYDRISFSST IVTEWDLVCD SQSLTSVAKF VFMAGMMVGG ILGGHLSDRF GRRFVLRWCY
LQVAIVGTCA ALAPTFLIYC SLRFLSGIAA MSLITNTIML IAEWATHRFQ AMGITLGMCP
SGIAFMTLAG LAFAIRDWHI LQLVVSVPYF VIFLTSSWLL ESARWLIINN KPEEGLKELR
KAAHRSGMKN ARDTLTLEIL KSTMKKELEA AQKKKPSLCE MLHMPNICKR ISLLSFTRFA
NFMAYFGLNL HVQHLGNNVF LLQTLFGAVI LLANCVAPWA LKYMNRRASQ MLLMFLLAIC
LLAIIFVPQE MQTLREVLAT LGLGASALAN TLAFAHGNEV IPTIIRARAM GINATFANIA
GALAPLMMIL SVYSPPLPWI IYGVFPFISG FAFLLLPETR NKPLFDTIQD EKNERKDPRE
PKQEDPRVEV TQF
